TOP TEN perturbations for AT1G08930 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT1G08930
Selected probe(set): 264624_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT1G08930 (264624_at) across 3240 perturbations tested by GENEVESTIGATOR:

germination (24h) / seed desiccation

Relative Expression (log2-ratio):4.618763
Number of Samples:3 / 3
Experimental germination (24h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 24h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

M6PR OX / Col-0

Relative Expression (log2-ratio):4.3362427
Number of Samples:2 / 2
Experimental M6PR OX
Transgenic line expressing celery (Apium graveolens) M6PR from the constitutive CaMV35S promoter. M6PR (NADPH-dependent mannose-6-phosphate reductase) converts mannose-6-phosphate to mannitol-1-phosphate. M6PR OX leaves produced approx. 3µmol mannitol per g FW (Col wild type does not produce any mannitol as Arabidopsis is species that does not produce mannitol). M6PR OX plants also produce 1-O-β-d-glucopyranosyl-D-mannitol (Zhifang and Loescher, 2003, Plant Cell Environ. 26: 275-283). Under standard growth chamber conditions M6PR OX plants are overtly similar to the Col wild type except that they have slightly greater leaf number, dry weight, and seed yield. M6PR OX plants are less susceptible to salt stress than the Col wild type: M6PR OX plants treated with 100mM NaCl from the 14th day after sowing on show less leaf chlorosis/necrosis and have greater leaf number, stalk number, plant height, dry weight, and seed yield compared to the wild type treated the same way (Chan et al., 2011, Plant Biotechnol. J.).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

shift 28°C to 19°C study 2 (35S:RPS4-HS) / 28°C (35S:RPS4-HS)

Relative Expression (log2-ratio):4.071684
Number of Samples:3 / 3
Experimental shift 28°C to 19°C study 2 (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 8h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

shift 28°C to 19°C (35S:RPS4-HS rrs1-11) / 28°C (35S:RPS4-HS rrs1-11)

Relative Expression (log2-ratio):4.058794
Number of Samples:3 / 3
Experimental shift 28°C to 19°C (35S:RPS4-HS rrs1-11)
Leaf samples of 35S:RPS4-HS rrs1-11 plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 2h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS rrs1-11)
Leaf samples of 35S:RPS4-HS rrs1-11 plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

germination (6h) / seed desiccation

Relative Expression (log2-ratio):3.9648743
Number of Samples:3 / 3
Experimental germination (6h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 6h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (12h) / seed desiccation

Relative Expression (log2-ratio):3.9322042
Number of Samples:3 / 3
Experimental germination (12h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 12h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

hypoxia study 6 (Col-0) / untreated plant samples (Col-0)

Relative Expression (log2-ratio):3.9077387
Number of Samples:2 / 2
Experimental hypoxia study 6 (Col-0)
Col-0 plants were placed in anaerobic microbial chambers and the chambers were flushed with a gas mixture of 0.1% Oxygen, balance Nitrogen at a flow rate of ~10L/min. for 20 minutes. Chambers were then sealed. Low oxygen treatment was performed for four hours in darkened chambers.
Control untreated plant samples (Col-0)
Untreated Col-0 plants.

germination (1h) / seed desiccation

Relative Expression (log2-ratio):3.8988028
Number of Samples:2 / 3
Experimental germination (1h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 1h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (48h) / seed desiccation

Relative Expression (log2-ratio):3.8159666
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

shift 28°C to 19°C (35S:RPS4-HS) / 28°C (35S:RPS4-HS)

Relative Expression (log2-ratio):3.803813
Number of Samples:3 / 3
Experimental shift 28°C to 19°C (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 2h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.