TOP TEN perturbations for AT1G11690 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT1G11690
Selected probe(set): 262812_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT1G11690 (262812_at) across 3240 perturbations tested by GENEVESTIGATOR:

cop1-4 / Col-0

Relative Expression (log2-ratio):-1.2856073
Number of Samples:3 / 3
Experimental cop1-4
Nonlethal, weak cop1 mutant that carries a nonsense mutation (Gln 283 → stop) in COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1; At2g32950). The cop1-4 mutant produces low amounts of truncated COP1 protein lacking the C-terminal 393 amino acids and thus lacking the WD40 repeats. COP1 is an E3 ubiquitin-protein ligase functioning in selective degradation of proteins, which control photomorphogenesis, photoperiodic growth, flowering time. Dark-grown cop1-4 seedlings exhibit light-grown characteristics (e.g. short hypocotyls; open cotyledons, which are larger than those of dark-grown Col-0 wild type). However, hypocotyls of dark-grown cop1-4 seedlings are longer than those of light-grown cop1-4 and light-grown Col-0 wild type (i.e. cop1-4 is still capable to react to light to a certain degree). Light-grown cop1-4 plants do not respond to photoperiod: they flower as if they were grown under long-day conditions (16h light / 8h darkness) regardless of the actual daylength (i.e. both long-day and short-day (8h light / 16h darkness) grown cop1-4 plants flower with approx. 7 rosette leaves whereas Col-0 wild type flowers with approx. 7 rosette leaves under long day and with approx. 44 rosette leaves under short-day conditions). In contrast to the wild type, cop1-4 shows neither the shade avoidance nor the end-of-day far-red response (McNellis et al, 1994, Plant Cell 6: 487-500; Chang et al., 2011, Plant Physiol. 156: 228-239). UVB (ultraviolet B) light response is impaired in cop1-4. When grown under white light (60μmol photons m-2 s-1) supplemented with UVB and UVA (>305nm; 1.6μmol m-2 s-1 irradiance at 312nm), cop1-4 plants become chlorotic while Col-0 wild type plants are green. Under white light of low intensity (3.6μmol m-2 s-1) supplemented with UVB and UVA (>305nm, 1.5μmol m-2 s-1), Col-0 wild type seedlings accumulate more flavonoids than under white light + UVA (>345nm, 5.1μmol m-2 s-1) whereas level of flavonoids does not differ in UVB-treated cop1-4 compared to UVB-untreated cop1-4 seedlings (Oravecz et al., 2006, Plant Cell 18: 1975-1990).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

uvr8-6 / Col-0

Relative Expression (log2-ratio):-1.2428489
Number of Samples:3 / 3
Experimental uvr8-6
Knockout mutant carrying T-DNA insertion (SALK_033468) in the 9th intron of UVR8 (UV RESPONSE LOCUS 8, At5g63860). UVR8 is a seven-bladed propeller protein acting in ultraviolet B light (UVB, 280-315nm) signalling via direct, UVB-dependent interaction with COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1, At2g32950). The uvr8-6 mutant is insensitive to UVB as a photomorphogenic signal: when grown under white light + UVA (>345nm) of low intensity (5.1μmol m-2 s-1), both Col-0 wild type and uvr8-6 seedlings have long hypocotyls, whereas under white light (3.6μmol m-2 s-1) supplemented with UVB and UVA (>305nm, 1.5μmol m-2 s-1) hypocotyls of wild type seedlings are shorter than those of uvr8-6 seedlings (hypocotyl length of UVB-treated and UVB-untreated uvr8-6 seedlings does not differ) (Favory et al., 2009, EMBO J. 28: 591-601).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

uvr8-6 / Col-0

Relative Expression (log2-ratio):-1.1909056
Number of Samples:3 / 3
Experimental uvr8-6
Knockout mutant carrying T-DNA insertion (SALK_033468) in the 9th intron of UVR8 (UV RESPONSE LOCUS 8, At5g63860). UVR8 is a seven-bladed propeller protein acting in ultraviolet B light (UVB, 280-315nm) signalling via direct, UVB-dependent interaction with COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1, At2g32950). The uvr8-6 mutant is insensitive to UVB as a photomorphogenic signal: when grown under white light + UVA (>345nm) of low intensity (5.1μmol m-2 s-1), both Col-0 wild type and uvr8-6 seedlings have long hypocotyls, whereas under white light (3.6μmol m-2 s-1) supplemented with UVB and UVA (>305nm, 1.5μmol m-2 s-1) hypocotyls of wild type seedlings are shorter than those of uvr8-6 seedlings (hypocotyl length of UVB-treated and UVB-untreated uvr8-6 seedlings does not differ) (Favory et al., 2009, EMBO J. 28: 591-601).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

cop1-4 / Col-0

Relative Expression (log2-ratio):-1.0608182
Number of Samples:3 / 3
Experimental cop1-4
Nonlethal, weak cop1 mutant that carries a nonsense mutation (Gln 283 → stop) in COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1; At2g32950). The cop1-4 mutant produces low amounts of truncated COP1 protein lacking the C-terminal 393 amino acids and thus lacking the WD40 repeats. COP1 is an E3 ubiquitin-protein ligase functioning in selective degradation of proteins, which control photomorphogenesis, photoperiodic growth, flowering time. Dark-grown cop1-4 seedlings exhibit light-grown characteristics (e.g. short hypocotyls; open cotyledons, which are larger than those of dark-grown Col-0 wild type). However, hypocotyls of dark-grown cop1-4 seedlings are longer than those of light-grown cop1-4 and light-grown Col-0 wild type (i.e. cop1-4 is still capable to react to light to a certain degree). Light-grown cop1-4 plants do not respond to photoperiod: they flower as if they were grown under long-day conditions (16h light / 8h darkness) regardless of the actual daylength (i.e. both long-day and short-day (8h light / 16h darkness) grown cop1-4 plants flower with approx. 7 rosette leaves whereas Col-0 wild type flowers with approx. 7 rosette leaves under long day and with approx. 44 rosette leaves under short-day conditions). In contrast to the wild type, cop1-4 shows neither the shade avoidance nor the end-of-day far-red response (McNellis et al, 1994, Plant Cell 6: 487-500; Chang et al., 2011, Plant Physiol. 156: 228-239). UVB (ultraviolet B) light response is impaired in cop1-4. When grown under white light (60μmol photons m-2 s-1) supplemented with UVB and UVA (>305nm; 1.6μmol m-2 s-1 irradiance at 312nm), cop1-4 plants become chlorotic while Col-0 wild type plants are green. Under white light of low intensity (3.6μmol m-2 s-1) supplemented with UVB and UVA (>305nm, 1.5μmol m-2 s-1), Col-0 wild type seedlings accumulate more flavonoids than under white light + UVA (>345nm, 5.1μmol m-2 s-1) whereas level of flavonoids does not differ in UVB-treated cop1-4 compared to UVB-untreated cop1-4 seedlings (Oravecz et al., 2006, Plant Cell 18: 1975-1990).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

cop1-4 / Col-0

Relative Expression (log2-ratio):-1.0282893
Number of Samples:3 / 3
Experimental cop1-4
Nonlethal, weak cop1 mutant that carries a nonsense mutation (Gln 283 → stop) in COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1; At2g32950). The cop1-4 mutant produces low amounts of truncated COP1 protein lacking the C-terminal 393 amino acids and thus lacking the WD40 repeats. COP1 is an E3 ubiquitin-protein ligase functioning in selective degradation of proteins, which control photomorphogenesis, photoperiodic growth, flowering time. Dark-grown cop1-4 seedlings exhibit light-grown characteristics (e.g. short hypocotyls; open cotyledons, which are larger than those of dark-grown Col-0 wild type). However, hypocotyls of dark-grown cop1-4 seedlings are longer than those of light-grown cop1-4 and light-grown Col-0 wild type (i.e. cop1-4 is still capable to react to light to a certain degree). Light-grown cop1-4 plants do not respond to photoperiod: they flower as if they were grown under long-day conditions (16h light / 8h darkness) regardless of the actual daylength (i.e. both long-day and short-day (8h light / 16h darkness) grown cop1-4 plants flower with approx. 7 rosette leaves whereas Col-0 wild type flowers with approx. 7 rosette leaves under long day and with approx. 44 rosette leaves under short-day conditions). In contrast to the wild type, cop1-4 shows neither the shade avoidance nor the end-of-day far-red response (McNellis et al, 1994, Plant Cell 6: 487-500; Chang et al., 2011, Plant Physiol. 156: 228-239). UVB (ultraviolet B) light response is impaired in cop1-4. When grown under white light (60μmol photons m-2 s-1) supplemented with UVB and UVA (>305nm; 1.6μmol m-2 s-1 irradiance at 312nm), cop1-4 plants become chlorotic while Col-0 wild type plants are green. Under white light of low intensity (3.6μmol m-2 s-1) supplemented with UVB and UVA (>305nm, 1.5μmol m-2 s-1), Col-0 wild type seedlings accumulate more flavonoids than under white light + UVA (>345nm, 5.1μmol m-2 s-1) whereas level of flavonoids does not differ in UVB-treated cop1-4 compared to UVB-untreated cop1-4 seedlings (Oravecz et al., 2006, Plant Cell 18: 1975-1990).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

uvr8-6 / Col-0

Relative Expression (log2-ratio):-1.0104489
Number of Samples:3 / 3
Experimental uvr8-6
Knockout mutant carrying T-DNA insertion (SALK_033468) in the 9th intron of UVR8 (UV RESPONSE LOCUS 8, At5g63860). UVR8 is a seven-bladed propeller protein acting in ultraviolet B light (UVB, 280-315nm) signalling via direct, UVB-dependent interaction with COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1, At2g32950). The uvr8-6 mutant is insensitive to UVB as a photomorphogenic signal: when grown under white light + UVA (>345nm) of low intensity (5.1μmol m-2 s-1), both Col-0 wild type and uvr8-6 seedlings have long hypocotyls, whereas under white light (3.6μmol m-2 s-1) supplemented with UVB and UVA (>305nm, 1.5μmol m-2 s-1) hypocotyls of wild type seedlings are shorter than those of uvr8-6 seedlings (hypocotyl length of UVB-treated and UVB-untreated uvr8-6 seedlings does not differ) (Favory et al., 2009, EMBO J. 28: 591-601).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

Ler-ttg / Ler-0

Relative Expression (log2-ratio):0.98455477
Number of Samples:2 / 3
Experimental Ler-ttg
Control Ler-0
Arabidopsis accession Landsberg erecta-0. ABRC stock No.: CS20. Origin: Poland.

IAA / FACS (pWOL::GFP) / root stele protoplast samples of mock treated pWOL::GFP

Relative Expression (log2-ratio):0.9363127
Number of Samples:3 / 3
Experimental IAA / FACS (pWOL::GFP)
Root stele protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWOL::GFP seedlings that had been grown for 7 days hydroponically in phytatrays with growth medium (0.5x Murashige and Skoog salts, 1% (w/v) sucrose, 0.5 g/l MES hydrate; pH 5.7), then transferred to 5μM IAA (indole-3-acetic acid) solution for 2h (total IAA treatment time was 3h as IAA was also present during the protoplast isolation and FACS sorting procedures). Other conditions: seedlings were grown in Advanced Intellus environmental controller (Percival), 18h light (35μmol photons m-2 s-1) / 6h dark, 22°C. FACS, Fluorescence Activated Cell Sorting.
Control root stele protoplast samples of mock treated pWOL::GFP
Root stele protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWOL::GFP seedlings that had been grown for 7 days hydroponically in phytatrays with growth medium (0.5x Murashige and Skoog salts, 1% (w/v) sucrose, 0.5 g/l MES hydrate; pH 5.7), then transferred to 0.05% (v/v) ethanol (solvent for IAA) for 2h (total mock-treatment time was 3h as ethanol was also present during the protoplast isolation and FACS sorting procedures). Other conditions: seedlings were grown in Advanced Intellus environmental controller (Percival), 18h light (35μmol photons m-2 s-1) / 6h dark, 22°C. FACS, Fluorescence Activated Cell Sorting.

Ler-ttg / Ler-0

Relative Expression (log2-ratio):0.87412214
Number of Samples:2 / 3
Experimental Ler-ttg
Control Ler-0
Arabidopsis accession Landsberg erecta-0. ABRC stock No.: CS20. Origin: Poland.

uvr8-6 / Col-0

Relative Expression (log2-ratio):-0.740726
Number of Samples:3 / 3
Experimental uvr8-6
Knockout mutant carrying T-DNA insertion (SALK_033468) in the 9th intron of UVR8 (UV RESPONSE LOCUS 8, At5g63860). UVR8 is a seven-bladed propeller protein acting in ultraviolet B light (UVB, 280-315nm) signalling via direct, UVB-dependent interaction with COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1, At2g32950). The uvr8-6 mutant is insensitive to UVB as a photomorphogenic signal: when grown under white light + UVA (>345nm) of low intensity (5.1μmol m-2 s-1), both Col-0 wild type and uvr8-6 seedlings have long hypocotyls, whereas under white light (3.6μmol m-2 s-1) supplemented with UVB and UVA (>305nm, 1.5μmol m-2 s-1) hypocotyls of wild type seedlings are shorter than those of uvr8-6 seedlings (hypocotyl length of UVB-treated and UVB-untreated uvr8-6 seedlings does not differ) (Favory et al., 2009, EMBO J. 28: 591-601).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).