TOP TEN perturbations for AT1G78800 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT1G78800
Selected probe(set): 264291_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT1G78800 (264291_at) across 3240 perturbations tested by GENEVESTIGATOR:

35S:RPS4-HS eds1-2 / 35S:RPS4-HS

Relative Expression (log2-ratio):-1.581624
Number of Samples:3 / 3
Experimental 35S:RPS4-HS eds1-2
Transgenic mutant line over-expressing Col-0 RPS4 (tagged with HA-StrepII) from the constitutive CaMV35S promoter and carrying fast neutron generated 939bp deletion in the coding region of the EDS1 (ENHANCED DISEASE SUSCEPTIBILITY1, At3g48090) gene (the deletion includes part of the exon 2, exon 3, part of the exon 4, and introns 2 and 3). RPS4 (At5g45250) is a TNL (Toll/Interleukin-1 (TIR) type nucleotide-binding domain leucine-rich repeat (NB-LRR)) receptor that recognizes bacterial Type III secreted effector AvrRps4. RPS4 is localized both at the membranes of endoplasmic reticulum and in the nucleus. EDS1 is a nucleus-cytoplasmic immune-regulator required for basal resistance against virulent pathogens and for TNL receptor-mediated effector-triggered immunity. Function of RPS4 depends on EDS1. 35S:RPS4-HS eds1-2 plants are similar to Col-0 wild type in terms of rosette diameter when grown at 19°C-22°C or 28°C for 3.5 weeks. At 19-22°C, 35S:RPS4-HS eds1-2 plants are slightly more sensitive to virulent Pseudomonas syringae pv. tomato strain DC3000 than the wild type and equally sensitive as the eds1-2 mutant without the 35S:RPS4-HS transgene (i.e. basal resistance of 35S:RPS4-HS eds1-2 is compromised). At 19-22°C, 35S:RPS4-HS eds1-2 is as sensitive to P. syringae pv. tomato strain DC3000 expressing AvrRps4 as eds1-2 whereas Col-0 wild type and 35S:RPS4-HS (plants with the transgene and wild type EDS1) are resistant. When grown at 28°C for 3.5 weeks and then shifted to 19°C, 35S:RPS4-HS eds1-2 plants, like the wild type, show neither leaf chlorosis nor cell death (Wirthmueller et al., 2007, Curr Biol. 17: 2023-2029; Heidrich et al., 2013, Front Plant Sci. 4: 403; Falk et al., 1999, PNAS 96: 3292-3297).
Control 35S:RPS4-HS
Transgenic line over-expressing Col-0 RPS4 (tagged with HA-StrepII) from the constitutive CaMV35S promoter. RPS4 (At5g45250) is a TNL (Toll/Interleukin-1 (TIR) type nucleotide-binding domain leucine-rich repeat (NB-LRR)) receptor that recognizes bacterial Type III secreted effector AvrRps4. RPS4 is localized both at the membranes of endoplasmic reticulum and in the nucleus. Presence of RPS4 in the nucleus is necessary for triggering the immune response but recognition of AvrRps4 does not change the distribution of RPS4. When grown at 19-22°C for 3.5 weeks, 35S:RPS4-HS plants are severely stunted (rosette diameter of 35S:RPS4-HS is reduced by approx. 62% compared to that of Col-0 wild type). At 19-22°C, 35S:RPS4-HS plants are more resistant to virulent Pseudomonas syringae pv. tomato strain DC3000 than the wild type (i.e. basal resistance is enhanced in 35S:RPS4-HS). At 19-22°C, 35S:RPS4-HS is slightly more resistant to P. syringae pv. tomato strain DC3000 expressing AvrRps4 than Col-0 wild type (although the wild type is also quite resistant as its endogenous RPS4 recognizes AvrRps4). When plants are grown at 28°C for 3.5 weeks, diameter of 35S:RPS4-HS rosettes is similar to that of wild type rosettes. When grown at 28°C for 3.5 weeks and then shifted to 19°C, 35S:RPS4-HS plants show leaf chlorosis and cell death (i.e. overexpression of RPS4-HS triggers temperature-dependent auto-immune response) (Wirthmueller et al., 2007, Curr Biol. 17: 2023-2029; Heidrich et al., 2013, Front Plant Sci. 4: 403).

heat study 2 (ws) / untreated leaf samples (ws)

Relative Expression (log2-ratio):-1.5604134
Number of Samples:2 / 2
Experimental heat study 2 (ws)
Wild type (ws) leaf samples after one hour heat treatment (37°C).
Control untreated leaf samples (ws)
Not available.

shift 28°C to 19°C study 2 (35S:RPS4-HS) / 28°C (35S:RPS4-HS)

Relative Expression (log2-ratio):1.5208607
Number of Samples:3 / 3
Experimental shift 28°C to 19°C study 2 (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 8h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

stratification (48h) / seed desiccation

Relative Expression (log2-ratio):1.493576
Number of Samples:3 / 3
Experimental stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

heat study 2 (hsf1/3) / untreated leaf samples (hsf1/3)

Relative Expression (log2-ratio):-1.4822845
Number of Samples:2 / 2
Experimental heat study 2 (hsf1/3)
hsf1:hsf3 leaf samples after one hour heat treatment (37°C).
Control untreated leaf samples (hsf1/3)
Not available.

sucrose study 5 (rgs1-2) / mock treated rgs1-2 guard cell samples

Relative Expression (log2-ratio):1.4702387
Number of Samples:3 / 3
Experimental sucrose study 5 (rgs1-2)
Guard cell pairs were manually dissected from rgs1-2 mature leaf blades that had been cut into 1mm wide strips, floated on a solution containing 150mM sucrose, 50mM KCl, 0.1mM CaCl2, 10mM MES-NaOH (pH 6.1), treated with vacuum (25 KPa) for 30s, incubated for 5h (22°C, 30μmol photons m-2 s-1) in the solution mentioned above, then blotted dry, frozen in liquid N2, freeze-dried for 4 days under vacuum of < 10mm of mercury, and stored under vacuum at 20°C. Before cutting the leaves, plants were grown for 8-10 weeks at 22°C, 60% relative humidity, under 8h light (150μmol photons m-2 s-1) / 16h dark cycles on Superfine Germinating Mix (FaFard).
Control mock treated rgs1-2 guard cell samples
Guard cell pairs were manually dissected from rgs1-2 mature leaf blades that had been cut into 1mm wide strips, floated on a solution containing 50mM KCl, 0.1mM CaCl2, 10mM MES-NaOH (pH 6.1), treated with vacuum (25 KPa) for 30s, incubated for 5h (22°C, 30μmol photons m-2 s-1) in the solution mentioned above, then blotted dry, frozen in liquid N2, freeze-dried for 4 days under vacuum of < 10mm of mercury, and stored under vacuum at 20°C. Before cutting the leaves, plants were grown for 8-10 weeks at 22°C, 60% relative humidity, under 8h light (150μmol photons m-2 s-1) / 16h dark cycles on Superfine Germinating Mix (FaFard).

shift 28°C to 19°C study 2 (35S:RPS4-HS rrs1-11) / 28°C (35S:RPS4-HS rrs1-11)

Relative Expression (log2-ratio):1.4486446
Number of Samples:3 / 3
Experimental shift 28°C to 19°C study 2 (35S:RPS4-HS rrs1-11)
Leaf samples of 35S:RPS4-HS rrs1-11 plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 8h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS rrs1-11)
Leaf samples of 35S:RPS4-HS rrs1-11 plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

germination (48h) / stratification (48h)

Relative Expression (log2-ratio):-1.4474335
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.

ozone study 5 (abi1td) / untreated rosette leaf samples (abi1td)

Relative Expression (log2-ratio):1.32199
Number of Samples:2 / 3
Experimental ozone study 5 (abi1td)
Rosette leaf samples of abi1td grown for 4 weeks on compost in a growth chamber, then exposed to 350ppb ozone for 6h. Other conditions: 16h light (300μmol photons m-2 s-1) / 8h dark cycles, 75% relative humidity, 21°C, the circulating air flow path; the plants were watered daily and fertilized with a nutrient solution once a week. O3 was produced from O2 using an electrical discharge.
Control untreated rosette leaf samples (abi1td)
Rosette leaf samples of abi1td grown for 4 weeks in a growth chamber on compost without water limitation, ozone-free. Other conditions: 16h light (300μmol photons m-2 s-1) / 8h dark cycles, 75% relative humidity, 21°C, the circulating air flow path; plants were watered daily and fertilized with a nutrient solution once a week.

salicylic acid study 13 (CS57549) / mock treated rosette leaf samples (CS57549)

Relative Expression (log2-ratio):1.2782955
Number of Samples:2 / 2
Experimental salicylic acid study 13 (CS57549)
Rosette leaf samples of CS57549 grown for 6 weeks in a growth chamber (8h light (100-120µmol photons m-2 s-1) / 16h darkness; 20°C), then sprayed to run-off with 0.3mM salicylic acid 0.02% Silwet L77 solution, and incubated for 28h.
Control mock treated rosette leaf samples (CS57549)
Rosette leaf samples of CS57549 grown for 6 weeks in a growth chamber (8h light (100-120µmol photons m-2 s-1) / 16h darkness; 20°C), then sprayed to run-off with 0.02% Silwet L77 solution, and incubated for 28h.