TOP TEN perturbations for AT1G80750 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT1G80750
Selected probe(set): 261911_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT1G80750 (261911_at) across 3240 perturbations tested by GENEVESTIGATOR:

salt / FACS (32h) / root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (1h)

Relative Expression (log2-ratio):2.572322
Number of Samples:3 / 3
Experimental salt / FACS (32h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on standard medium supplemented with 140mM NaCl for 32h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.
Control root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (1h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on fresh standard medium for 1h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.

salt / FACS (48h) / root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (48h)

Relative Expression (log2-ratio):2.501708
Number of Samples:3 / 3
Experimental salt / FACS (48h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on standard medium supplemented with 140mM NaCl for 48h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.
Control root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (48h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on fresh standard medium for 48h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.

stratification (48h) / seed desiccation

Relative Expression (log2-ratio):2.481659
Number of Samples:3 / 3
Experimental stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

salt / FACS study 2 (32h) / root cortex protoplast samples of mock treated pCOR315.1::GFP (1h)

Relative Expression (log2-ratio):2.4770212
Number of Samples:3 / 3
Experimental salt / FACS study 2 (32h)
Root cortex protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pCOR315.1::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on standard medium supplemented with 140mM NaCl for 32h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.
Control root cortex protoplast samples of mock treated pCOR315.1::GFP (1h)
Root cortex protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pCOR315.1::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on fresh standard medium for 1h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.

two-cycle RNA labeling (root elongation zone) / two-cycle RNA labeling (root tip)

Relative Expression (log2-ratio):-2.4478579
Number of Samples:3 / 3
Experimental two-cycle RNA labeling (root elongation zone)
Root elongation zone samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix Two-Cycle Eukaryotic Target Labeling Assay kit.
Control two-cycle RNA labeling (root tip)
Root tip samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix Two-Cycle Eukaryotic Target Labeling Assay kit.

germination (1h) / seed desiccation

Relative Expression (log2-ratio):2.4279194
Number of Samples:2 / 3
Experimental germination (1h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 1h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

shift NPA to NAA (6h) / NPA study 3

Relative Expression (log2-ratio):2.4153185
Number of Samples:3 / 3
Experimental shift NPA to NAA (6h)
Primary root samples of Col-0 grown for 3 days on vertically oriented plates containing solid Murashige-Skoog medium supplemented with 10µM NPA (1-N-naphthylphthalamic acid, an inhibitor of auxin transport), then transferred to 10µM NAA (1-naphthalene acetic acid, synthetic auxin) for 6h.
Control NPA study 3
Primary root samples of Col-0 grown for 3 days on vertically oriented plates containing solid Murashige-Skoog medium supplemented with 10µM NPA (1-N-naphthylphthalamic acid, an inhibitor of auxin transport).

germination (48h) / stratification (48h)

Relative Expression (log2-ratio):-2.3330145
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.

one-cycle RNA labeling (root elongation zone) / one-cycle RNA labeling (root tip)

Relative Expression (log2-ratio):-2.1588478
Number of Samples:3 / 3
Experimental one-cycle RNA labeling (root elongation zone)
Root elongation zone samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix One-Cycle Eukaryotic Target Labeling Assay kit.
Control one-cycle RNA labeling (root tip)
Root tip samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix One-Cycle Eukaryotic Target Labeling Assay kit.

salt / FACS (48h) / root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (1h)

Relative Expression (log2-ratio):2.0174618
Number of Samples:3 / 3
Experimental salt / FACS (48h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on standard medium supplemented with 140mM NaCl for 48h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.
Control root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (1h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on fresh standard medium for 1h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.