TOP TEN perturbations for AT2G24210 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT2G24210
Selected probe(set): 265984_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT2G24210 (265984_at) across 3240 perturbations tested by GENEVESTIGATOR:

MeJa study 5 (penta) / untreated leaf disc samples (penta)

Relative Expression (log2-ratio):6.9625144
Number of Samples:3 / 3
Experimental MeJa study 5 (penta)
Leaf disc samples were collected from five weeks old penta mutant plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions, sprayed with 10µM Methyl Jasmonate solution, covered with plastic bags and kept for one hour.
Control untreated leaf disc samples (penta)
Leaf disc samples of five weeks old penta mutant plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions.

sid2 / coi1

Relative Expression (log2-ratio):6.9112015
Number of Samples:3 / 3
Experimental sid2
Mutant (AT1G74710) which lacks an enzyme required for SA biosynthesis. SID2 protein participates in basal disease resistance to virulent pathogens as well as R protein-mediated resistance to avirulent pathogens.
Control coi1
Mutant allele coi1 (At2g39940, Coronatine-Insensitive 1) conferred male sterility, insensitivity to the phytotoxin coronatine, and insensitivity to the phytohormone MeJA.

arf6-2 arf8-3 / Col

Relative Expression (log2-ratio):-6.8077602
Number of Samples:3 / 3
Experimental arf6-2 arf8-3
Double knockout mutant carrying T-DNA insertions in the 12th exon of ARF6 (Auxin Response Factor 6; At1g30330) and in the 10th intron of ARF8 (Auxin Response Factor 8; At5g37020). ARF6 and ARF8 are transcription factors that coordinate the transition from immature to mature fertile flowers. At seedling and early vegetative growth stage arf6-2 arf8-3 plants are overtly similar to Col wild type. At flowering arf6-2 arf8-3 inflorescence stems elongate less than those of the wild type. arf6-2 arf8-3 flowers arrest as infertile closed buds with short petals, immature pistils with short stigmatic papillae, short stamen filaments, and undehisced anthers that do not release pollen. Col wild type flowers produce jasmonic acid (JA) and JA level is highest in the oldest closed buds while in arf6-2 arf8-3 flowers JA is undetectable. Feeding with exogenous JA partially rescues the arf6-2 arf8-3 phenotype: mutant anthers dehisce, pollen are released, petals elongate, and flowers open slightly; however, stamen filaments remain short and pistil development is not restored (Nagpal et al., 2005, Development 132: 4107-4118).
Control Col
Arabidopsis ecotype Columbia. Origin: Poland. Altitude of the origin ≈ 200m (Duruflé et al., 2019, Data Brief. 25: 104318).

npr1-1 / coi1

Relative Expression (log2-ratio):6.745778
Number of Samples:3 / 3
Experimental npr1-1
EMS-generated mutant carrying missense mutation (highly conserved His 334 → Tyr) in NPR1 (Non expresser of PR (pathogenesis-related) genes; At1g64280). NPR1 controls the onset of systemic acquired resistance (SAR) to a broad spectrum of pathogens after a primary exposure to avirulent pathogens. Compared to Col-0 wild type, npr1-1 is more susceptible to Pseudomonas syringae pv. maculicola ES4326 (Psm ES4326), bacterial pathogen virulent on Col-0 wild type. In Col-0 wild type plants first treated with 1mM salicylic acid (SA) or 0.65mM 2,6-dichloroiso-nicotinic acid (INA) and then, 2 days later, exposed to Psm ES4326, growth of PsmES4326 is inhibited by more than 99% compared to growth of the bacteria in mock-pretreated plants (SA and INA induce the systemic acquired resistance (SAR) in the wild type). SA or INA pretreated npr1-1 plants are almost as susceptible to Psm ES4326 as mock-pretreated npr1-1 plants (SAR is not induced in npr1-1 by SA or INA). Pretreatment with Pseudomonas syringae pv. phaseolicola NPS3121/avrRpt2, bacterial pathogen avirulent on both Col-0 wild type and npr1-1, induces SAR in the wild type but not in npr1-1 (Cao et al., 1994, Plant Cell 6: 1583-1592; Cao et al., 1997, Cell 88: 57-63).
Control coi1
Mutant allele coi1 (At2g39940, Coronatine-Insensitive 1) conferred male sterility, insensitivity to the phytotoxin coronatine, and insensitivity to the phytohormone MeJA.

pad4-1 / coi1

Relative Expression (log2-ratio):6.412594
Number of Samples:3 / 3
Experimental pad4-1
EMS-generated mutant carrying nonsense mutation (Trp 359 → stop) in PAD4 (Phytoalexin deficient 4, At3g52430). PAD4 is a lipase-like protein involved in resistance (R) gene-mediated effector-triggered immunity, basal immunity, and nonhost resistance. PAD4 directly interacts with EDS1 (encoded by At3g48090), PAD4-EDS1 complex is localized both in the nucleus and in the cytosol. When plants are inoculated with Pseudomonas syringae pv. maculicola ES4326 (PsmES4326; virulent on Col-0 wild type), accumulation of camalexin is reduced by approx. 90% in pad4-1 compared to Col-0 wild type. Compared to the wild type, pad4-1 is more susceptible to PsmES4326. Both pad4-1 and wild type plants become less susceptible to PsmES4326 when they are pretreated with 5mM salicylic acid (compared to mock-pretreated plants). Compared to the wild type, pad4-1 is only slightly less resistant to PsmES4326 avrRpt2 (PsmES4326 that expresses avirulence gene avrRpt2 and is thus avirulent on Col-0, which contains the resistance gene RPS2). After inoculation with PsmES4326, pad4-1 accumulates less salicylic acid (SA) than the wild type but after inoculation with PsmES4326 avrRpt2 both pad4-1 and the wild type accumulate similar amounts of SA and camalexin. The pad4-1 mutant is as susceptible to Xanthomonas campestris pv. campestris BP109 as the wild type (Jirage et al., 1999, PNAS 96: 13583-13588; Glazebrook et al., 1997, Genetics 146: 381-392; Zhou et al., 1998, Plant Cell 10: 1021-1030).
Control coi1
Mutant allele coi1 (At2g39940, Coronatine-Insensitive 1) conferred male sterility, insensitivity to the phytotoxin coronatine, and insensitivity to the phytohormone MeJA.

MeJa study 5 (Ler) / untreated leaf disc samples (Ler)

Relative Expression (log2-ratio):6.292737
Number of Samples:3 / 3
Experimental MeJa study 5 (Ler)
Leaf disc samples were collected from five weeks old Ler plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions, sprayed with 10µM Methyl Jasmonate solution, covered with plastic bags and kept for one hour.
Control untreated leaf disc samples (Ler)
Leaf disk samples of five weeks old Ler plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions.

MeJa (late) / opr3 stamen samples (0h)

Relative Expression (log2-ratio):6.0728436
Number of Samples:3 / 3
Experimental MeJa (late)
Stamens of opr3 mutants were treated with 0.03% methyl jasmonate and stamens were harvested 22h after treatment.
Control opr3 stamen samples (0h)
Stamens of opr3 mutants were treated with 0.03% methyl jasmonate and stamens were harvested at several time-point 0h after treatment (=control of time series).

sid2 / ein2

Relative Expression (log2-ratio):5.3404503
Number of Samples:3 / 3
Experimental sid2
Mutant (AT1G74710) which lacks an enzyme required for SA biosynthesis. SID2 protein participates in basal disease resistance to virulent pathogens as well as R protein-mediated resistance to avirulent pathogens.
Control ein2
The ein2 mutant (AT5G03280) is insensitive to ethylene.

npr1-1 / ein2

Relative Expression (log2-ratio):5.175027
Number of Samples:3 / 3
Experimental npr1-1
EMS-generated mutant carrying missense mutation (highly conserved His 334 → Tyr) in NPR1 (Non expresser of PR (pathogenesis-related) genes; At1g64280). NPR1 controls the onset of systemic acquired resistance (SAR) to a broad spectrum of pathogens after a primary exposure to avirulent pathogens. Compared to Col-0 wild type, npr1-1 is more susceptible to Pseudomonas syringae pv. maculicola ES4326 (Psm ES4326), bacterial pathogen virulent on Col-0 wild type. In Col-0 wild type plants first treated with 1mM salicylic acid (SA) or 0.65mM 2,6-dichloroiso-nicotinic acid (INA) and then, 2 days later, exposed to Psm ES4326, growth of PsmES4326 is inhibited by more than 99% compared to growth of the bacteria in mock-pretreated plants (SA and INA induce the systemic acquired resistance (SAR) in the wild type). SA or INA pretreated npr1-1 plants are almost as susceptible to Psm ES4326 as mock-pretreated npr1-1 plants (SAR is not induced in npr1-1 by SA or INA). Pretreatment with Pseudomonas syringae pv. phaseolicola NPS3121/avrRpt2, bacterial pathogen avirulent on both Col-0 wild type and npr1-1, induces SAR in the wild type but not in npr1-1 (Cao et al., 1994, Plant Cell 6: 1583-1592; Cao et al., 1997, Cell 88: 57-63).
Control ein2
The ein2 mutant (AT5G03280) is insensitive to ethylene.

arf6-2 arf8-3 / Col

Relative Expression (log2-ratio):-5.0838223
Number of Samples:3 / 3
Experimental arf6-2 arf8-3
Double knockout mutant carrying T-DNA insertions in the 12th exon of ARF6 (Auxin Response Factor 6; At1g30330) and in the 10th intron of ARF8 (Auxin Response Factor 8; At5g37020). ARF6 and ARF8 are transcription factors that coordinate the transition from immature to mature fertile flowers. At seedling and early vegetative growth stage arf6-2 arf8-3 plants are overtly similar to Col wild type. At flowering arf6-2 arf8-3 inflorescence stems elongate less than those of the wild type. arf6-2 arf8-3 flowers arrest as infertile closed buds with short petals, immature pistils with short stigmatic papillae, short stamen filaments, and undehisced anthers that do not release pollen. Col wild type flowers produce jasmonic acid (JA) and JA level is highest in the oldest closed buds while in arf6-2 arf8-3 flowers JA is undetectable. Feeding with exogenous JA partially rescues the arf6-2 arf8-3 phenotype: mutant anthers dehisce, pollen are released, petals elongate, and flowers open slightly; however, stamen filaments remain short and pistil development is not restored (Nagpal et al., 2005, Development 132: 4107-4118).
Control Col
Arabidopsis ecotype Columbia. Origin: Poland. Altitude of the origin ≈ 200m (Duruflé et al., 2019, Data Brief. 25: 104318).