TOP TEN perturbations for AT2G32390 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT2G32390
Selected probe(set): 266337_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT2G32390 (266337_at) across 3240 perturbations tested by GENEVESTIGATOR:

shift low light to dark to high light (RNAi-MBS2 mbs1-1) / shift low light to dark (RNAi-MBS2 mbs1-1)

Relative Expression (log2-ratio):2.1309252
Number of Samples:3 / 3
Experimental shift low light to dark to high light (RNAi-MBS2 mbs1-1)
Shoot samples of 12-day-old RNAi-MBS2 mbs1-1 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h, and then exposed to high light (1000µmol photons m-2 s-1) for 3h.
Control shift low light to dark (RNAi-MBS2 mbs1-1)
Shoot samples of 12-day-old RNAi-MBS2 mbs1-1 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h.

shift low to high light study 4 (Col-0) / low light study 5 (Col-0)

Relative Expression (log2-ratio):2.1100883
Number of Samples:3 / 3
Experimental shift low to high light study 4 (Col-0)
Rosette samples of Col-0 plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days, and then shifted to high light (800µmol m-2 s-1) for 6h. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).
Control low light study 5 (Col-0)
Rosette samples of Col-0 plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days and 6h. For the last 6h the plants were kept in light. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).

shift 28°C to 19°C (35S:RPS4-HS eds1-2) / 28°C (35S:RPS4-HS eds1-2)

Relative Expression (log2-ratio):2.0852842
Number of Samples:3 / 3
Experimental shift 28°C to 19°C (35S:RPS4-HS eds1-2)
Leaf samples of 35S:RPS4-HS eds1-2 plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 2h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS eds1-2)
Leaf samples of 35S:RPS4-HS eds1-2 plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

shift low light to dark to high light (Col-0) / shift low light to dark (Col-0)

Relative Expression (log2-ratio):2.0681162
Number of Samples:3 / 2
Experimental shift low light to dark to high light (Col-0)
Shoot samples of 12-day-old Col-0 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h, and then exposed to high light (1000µmol photons m-2 s-1) for 3h.
Control shift low light to dark (Col-0)
Shoot samples of 12-day-old Col-0 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h.

shift low light to dark to high light (35S:MBS1) / shift low light to dark (35S:MBS1)

Relative Expression (log2-ratio):1.9532385
Number of Samples:3 / 3
Experimental shift low light to dark to high light (35S:MBS1)
Shoot samples of 12-day-old 35S:MBS1 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h, and then exposed to high light (1000µmol photons m-2 s-1) for 3h.
Control shift low light to dark (35S:MBS1)
Shoot samples of 12-day-old 35S:MBS1 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h.

shift low to high light study 4 (rap2.4a) / low light study 5 (rap2.4a)

Relative Expression (log2-ratio):1.9314651
Number of Samples:3 / 3
Experimental shift low to high light study 4 (rap2.4a)
Rosette samples of rap2.4a plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days, and then shifted to high light (800µmol m-2 s-1) for 6h. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).
Control low light study 5 (rap2.4a)
Rosette samples of rap2.4a plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days and 6h. For the last 6h the plants were kept in light. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).

shift 28°C to 19°C (35S:RPS4-HS) / 28°C (35S:RPS4-HS)

Relative Expression (log2-ratio):1.8852959
Number of Samples:3 / 3
Experimental shift 28°C to 19°C (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 2h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

shift low light to dark to high light (mbs1-1) / shift low light to dark (mbs1-1)

Relative Expression (log2-ratio):1.8274002
Number of Samples:2 / 3
Experimental shift low light to dark to high light (mbs1-1)
Shoot samples of 12-day-old mbs1-1 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h, and then exposed to high light (1000µmol photons m-2 s-1) for 3h.
Control shift low light to dark (mbs1-1)
Shoot samples of 12-day-old mbs1-1 plants grown on solid 0.5x Murashige and Skoog medium with 1% sucrose under continuous light (for the last 7 days at 10µmol photons m-2 s-1), then shifted to darkness for 3h.

heat study 4 / untreated plant samples

Relative Expression (log2-ratio):-1.6843834
Number of Samples:2 / 2
Experimental heat study 4
Plant samples, grown in vitro under long day conditions at 22°C for three weeks, then exposed to 37°C for 30h (heat exposure started 3h after beginning of the light phase).
Control untreated plant samples
Plant samples, grown in vitro under long day conditions at 22°C for three weeks followed by additional 30h at the same temperature.

shift low to high light (adg1-1) / low light study 3 (adg1-1)

Relative Expression (log2-ratio):1.6802368
Number of Samples:2 / 2
Experimental shift low to high light (adg1-1)
Rosette samples of adg1-1 plants grown for 3 weeks on soil under 16h low light (30µmol photons m-2 s-1) at 22°C / 8h dark at 18°C cycles, then exposed to high light (300µmol photons m-2 s-1) at 22°C for 4h. The plants were shifted to high light 3.5h after the beginning of the light period.
Control low light study 3 (adg1-1)
Rosette samples of adg1-1 plants grown for 3 weeks on soil under 16h low light (30µmol photons m-2 s-1) at 22°C / 8h dark at 18°C cycles. The samples were harvested 3.5h after the beginning of the light period.