TOP TEN perturbations for AT3G04080 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT3G04080
Selected probe(set): 258567_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT3G04080 (258567_at) across 3240 perturbations tested by GENEVESTIGATOR:

germination (24h) / seed desiccation

Relative Expression (log2-ratio):3.6986256
Number of Samples:3 / 3
Experimental germination (24h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 24h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (48h) / seed desiccation

Relative Expression (log2-ratio):3.3002758
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (12h) / seed desiccation

Relative Expression (log2-ratio):2.5771885
Number of Samples:3 / 3
Experimental germination (12h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 12h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (24h) / stratification (48h)

Relative Expression (log2-ratio):2.3678322
Number of Samples:3 / 3
Experimental germination (24h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 24h.
Control stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.

heat study 2 (hsf1/3) / untreated leaf samples (hsf1/3)

Relative Expression (log2-ratio):-2.3510284
Number of Samples:2 / 2
Experimental heat study 2 (hsf1/3)
hsf1:hsf3 leaf samples after one hour heat treatment (37°C).
Control untreated leaf samples (hsf1/3)
Not available.

heat study 2 (ws) / untreated leaf samples (ws)

Relative Expression (log2-ratio):-2.3176575
Number of Samples:2 / 2
Experimental heat study 2 (ws)
Wild type (ws) leaf samples after one hour heat treatment (37°C).
Control untreated leaf samples (ws)
Not available.

germination (48h) / stratification (48h)

Relative Expression (log2-ratio):1.9694824
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.

salt / FACS (48h) / root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (1h)

Relative Expression (log2-ratio):-1.888586
Number of Samples:3 / 3
Experimental salt / FACS (48h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on standard medium supplemented with 140mM NaCl for 48h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.
Control root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP (1h)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 5 days on a mesh placed on top of standard medium, then transferred (together with the mesh) on fresh standard medium for 1h. Standard medium: 1x Murashige and Skoog salts, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7; prior protoplasting the seedlings were kept under 16h light / 8h dark cycles. FACS, Fluorescence Activated Cell Sorting.

germination (6h) / seed desiccation

Relative Expression (log2-ratio):1.6732645
Number of Samples:3 / 3
Experimental germination (6h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 6h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

P. syringae pv. tomato study 9 (DC3118 Cor-) / mock inoculated leaf samples

Relative Expression (log2-ratio):1.5196085
Number of Samples:3 / 2
Experimental P. syringae pv. tomato study 9 (DC3118 Cor-)
Arabidopsis Col-5 leaf samples from plants, inoculated with Pseudomonas syringae pv. tomato mutant DC3118 Coronatine- bacteria at a concentration of 5x10e7 cfu/ml for 10h.
Control mock inoculated leaf samples
Arabidopsis Col-5 leaf samples from plants, inoculated with a mock inoculum of distilled/deionized water for 10h.