TOP TEN perturbations for AT3G56400 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT3G56400
Selected probe(set): 251705_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT3G56400 (251705_at) across 3240 perturbations tested by GENEVESTIGATOR:

bleomycin + INA / untreated seedling samples

Relative Expression (log2-ratio):5.7666216
Number of Samples:3 / 3
Experimental bleomycin + INA
Seedling samples of Arabidopsis wild type grown for 9 days on solid Murashige and Skoog medium supplemented with 4μg/ml bleomycin (a DNA-damaging agent) and 10μM INA (6-dichloroisonicotinic acid, an analog of salicylic acid). Other conditions: 16h light / 8h dark cycles, 21-24°C.
Control untreated seedling samples
Seedling samples of Arabidopsis wild type grown for 9 days on solid Murashige and Skoog medium under 16h light / 8h dark cycles at 21-24°C.

P. parasitica (30h) / non-infected root samples (Col-0)

Relative Expression (log2-ratio):5.5791826
Number of Samples:2 / 2
Experimental P. parasitica (30h)
Root samples of Col-0 plants grown first for 15 days on agar medium (1xMS, 1% sucrose, 2% agar) then for 1 month in new Petri dishes containing a strip of 1xMS agar medium and underneath 10 ml of liquid 0.1xMS medium, under short day (8h light / 16h dark), 25°C. Roots were then inoculated with Phytophthora parasitica 310 strain (10^6 zoospores per Petri dish with 10 plants) and collected after 30h.
Control non-infected root samples (Col-0)
Root samples of Col-0 plants grown first for 15 days on agar medium (1xMS, 1% sucrose, 2% agar) then for 1 month in new Petri dishes containing a strip of 1xMS agar medium and underneath 10 ml of liquid 0.1xMS medium, under short day (8h light / 16h dark), 25°C.

dicamba (10h) / H2O treated seedling samples (10h)

Relative Expression (log2-ratio):5.3920956
Number of Samples:3 / 3
Experimental dicamba (10h)
Seedling samples of line GSTF8::LUC grown for 4 days on solid MS medium containing 3% sucrose under 16h light / 8h dark cycles at 22°C, then flooded with 7mM dicamba solution for 40min and, after removing the excess of the dicamba solution, incubated for 9h 20min. Dicamba is a herbicide that mimics the natural auxin indole-3-acetic acid (concentration used in this experiment falls within the range used for weed control).
Control H2O treated seedling samples (10h)
Seedling samples of line GSTF8::LUC grown for 4 days on solid MS medium containing 3% sucrose under 16h light / 8h dark cycles at 22°C, then flooded with water for 40min and, after removing the excess water, incubated for 9h 20min.

salicylic acid study 11 (dsr1) / mock treated seedling samples (dsr1)

Relative Expression (log2-ratio):5.370694
Number of Samples:3 / 3
Experimental salicylic acid study 11 (dsr1)
Seedling samples of dsr1 grown for 4 days on plates (56mm diameter) with solid Murashige and Skoog medium (16h light / 8h dark; 21°C), then treated with salicylic acid (SA) by pouring 5ml of 1mM SA solution into the plates with the seedlings. 40min after the treatment excess liquid was discarded and the seedlings were grown for further 9h 20min.
Control mock treated seedling samples (dsr1)
Seedling samples of dsr1 grown for 4 days on plates (56mm diameter) with solid Murashige and Skoog medium (16h light / 8h dark; 21°C), then mock treated by pouring 5ml water into the plates with the seedlings. 40min after the treatment excess liquid was discarded and the seedlings were grown for further 9h 20min.

salicylic acid study 11 (JC66) / mock treated seedling samples (JC66)

Relative Expression (log2-ratio):5.2773447
Number of Samples:3 / 3
Experimental salicylic acid study 11 (JC66)
Seedling samples of JC66 grown for 4 days on plates (56mm diameter) with solid Murashige and Skoog medium (16h light / 8h dark; 21°C), then treated with salicylic acid (SA) by pouring 5ml of 1mM SA solution into the plates with the seedlings. 40min after the treatment excess liquid was discarded and the seedlings were grown for further 9h 20min.
Control mock treated seedling samples (JC66)
Seedling samples of JC66 grown for 4 days on plates (56mm diameter) with solid Murashige and Skoog medium (16h light / 8h dark; 21°C), then mock treated by pouring 5ml water into the plates with the seedlings. 40min after the treatment excess liquid was discarded and the seedlings were grown for further 9h 20min.

cycloheximide / mock treated seedlings

Relative Expression (log2-ratio):5.2260637
Number of Samples:2 / 2
Experimental cycloheximide
Cycloheximide (protein synthesis inhibitor); 10uM CHX for 3h.
Control mock treated seedlings
Mock treatment for 3h.

salicylic acid / mock treated seedlings

Relative Expression (log2-ratio):5.0865574
Number of Samples:2 / 2
Experimental salicylic acid
10uM salicylic acid for 3h.
Control mock treated seedlings
Mock treatment for 3h.

UV unfiltered max-310nm (6h) / seedlings irradiated with 327nm cut-off (6h)

Relative Expression (log2-ratio):4.9090347
Number of Samples:2 / 2
Experimental UV unfiltered max-310nm (6h)
Seedlings of Arabidopsis were irradiated for 15 minutes at midday with UV-B from Philips TL 40W/12 UV fluorescent tubes (Lambda,max = 310 nm, half-bandwidth = 40 nm, fluence rate = 7 W/m2) unfiltered through a 3-mm quartz plate. Samples were taken 6 h after the start of irradiation.
Control seedlings irradiated with 327nm cut-off (6h)
Seedlings of Arabidopsis were irradiated for 15 minutes at midday with UV-B from Philips TL 40W/12 UV fluorescent tubes (Lambda,max = 310 nm, half-bandwidth = 40 nm, fluence rate = 7 W/m2) filtered through 3-mm transmission cut-off filters of the WG series with half-maximal transmission at 327 nm (WG327 represents the minus UV-B control). Samples were taken 6 h after the start of irradiation.

shift 28°C to 19°C study 2 (35S:RPS4-HS rrs1-11) / 28°C (35S:RPS4-HS rrs1-11)

Relative Expression (log2-ratio):4.632243
Number of Samples:3 / 3
Experimental shift 28°C to 19°C study 2 (35S:RPS4-HS rrs1-11)
Leaf samples of 35S:RPS4-HS rrs1-11 plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 8h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS rrs1-11)
Leaf samples of 35S:RPS4-HS rrs1-11 plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

salicylic acid study 9 (24h) / solvent treated cell samples (24h)

Relative Expression (log2-ratio):4.523717
Number of Samples:2 / 2
Experimental salicylic acid study 9 (24h)
Cell samples of T87 cell suspension that was grown in modified JPL medium (Axelos et al., 1992, Plant Physiol Biochem. 30: 123-128) under 16h light / 8h dark cycles, 24°C, on a rotary shaker at 100rpm with subculturing every 7 days and then, at the exponential phase of growth, exposed to 300μM salicylic acid for 24h.
Control solvent treated cell samples (24h)
Cell samples of T87 cell suspension that was grown in modified JPL medium (Axelos et al., 1992, Plant Physiol Biochem. 30: 123-128) under 16h light / 8h dark cycles, 24°C, on a rotary shaker at 100rpm with subculturing every 7 days and then, at the exponential phase of growth, exposed to 0.6% ethanol for 24h.