TOP TEN perturbations for AT5G41370 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT5G41370
Selected probe(set): 249307_s_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT5G41370 (249307_s_at) across 3240 perturbations tested by GENEVESTIGATOR:

bzr1-1Dxbri1-116 / Col-0

Relative Expression (log2-ratio):2.820417
Number of Samples:3 / 3
Experimental bzr1-1Dxbri1-116
bzr1-1Dbri1-116 double mutant in Col-0 background. bzr1-1D is a dominant mutation which stabilizes the BZR1 protein. The de-etiolated phenotype of the null bri1-116 mutant grown in the dark is completely suppressed by the bzr1-1D mutation.
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

bzr1-1Dxbri1-116 / bri1-116

Relative Expression (log2-ratio):2.7691283
Number of Samples:3 / 3
Experimental bzr1-1Dxbri1-116
bzr1-1Dbri1-116 double mutant in Col-0 background. bzr1-1D is a dominant mutation which stabilizes the BZR1 protein. The de-etiolated phenotype of the null bri1-116 mutant grown in the dark is completely suppressed by the bzr1-1D mutation.
Control bri1-116
bri1-116 null mutant in Col-0 background. bri1-116 is a severe BR-perception mutant.

ABA study 8 (Col-0) / solvent treated leaf samples (Col-0)

Relative Expression (log2-ratio):-2.1025429
Number of Samples:3 / 3
Experimental ABA study 8 (Col-0)
Excised leaf samples of 5 weeks old Arabidopsis thaliana Col-0 wild-type plants, treated with 50mM ABA for 3h.
Control solvent treated leaf samples (Col-0)
Mature leaf samples of 5 weeks old Arabidopsis thaliana Col-0 wild-type plants, treated with EtOH for 3h.

iron deficiency / protoplasting / iron deficiency study 8 (24h)

Relative Expression (log2-ratio):-2.0215826
Number of Samples:2 / 3
Experimental iron deficiency / protoplasting
Col-0 seedlings were grown for 5 days on iron-sufficient medium (1x Murashige and Skoog salt mixture in which ferrous sulfate was replaced with 100mM Fe(III)-EDTA, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7), then transferred to iron deficient medium (1x Murashige and Skoog salt mixture in which ferrous sulfate was replaced with 300μM Ferrozine, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7) for 24h; protoplasts were then isolated from the whole roots and FACS-sorted (all protoplasts were taken after the sorting). FACS, Fluorescence Activated Cell Sorting.
Control iron deficiency study 8 (24h)
Whole root samples of Col-0 seedlings grown on iron-sufficient medium (1x Murashige and Skoog salt mixture in which ferrous sulfate was replaced with 100mM Fe(III)-EDTA, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7) for 5 days, then transferred to iron deficient medium (1x Murashige and Skoog salt mixture in which ferrous sulfate was replaced with 300μM Ferrozine, 0.5g/L MES, 1% sucrose, 1% agar; pH 5.7) for 24h.

syringolin study 3 (late) / solvent treated leaf samples (Col-0; late)

Relative Expression (log2-ratio):1.901824
Number of Samples:3 / 3
Experimental syringolin study 3 (late)
Primary leaf samples of uninfected Col-0 plants, treated with Syringolin A (sylA) solution (20 uM) for 8h to 12h.
Control solvent treated leaf samples (Col-0; late)
Primary leaf samples of uninfected Col-0 plants, treated with control buffer solution (Tween 20 0.05%) for 8h to 12h.

oxt6:AtCPSF30 / Col-0

Relative Expression (log2-ratio):1.8778429
Number of Samples:4 / 3
Experimental oxt6:AtCPSF30
Oxt6 (polyadenylation factor subunit) transformant that carries a gene encoding just the smaller of the two At1g30460-encoded proteins.
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

rrd2 / Ler

Relative Expression (log2-ratio):-1.8059931
Number of Samples:3 / 3
Experimental rrd2
EMS-generated mutant rrd2 (root redifferentiation 2). The RRD2 gene was mapped on chromosome I, between the genetic markers UFO and GAPB. When hypocotyl explants of rrd2 are placed on root inducing medium (RIM) at 28°C, adventitious roots are formed but they are morphologically abnormal (root apex is bell-shaped, swelled, with unusually vacuolated cells). At 22°C on RIM, rrd2 hypocotyl explants form adventitious roots that are very similar to roots produced by Ler wild type hypocotyl explants at both 22°C and 28°C. When root explants are cultured on RIM at 28°C, lateral root primordia of rrd2 have much more cells along the base and are thus much wider than lateral root primordia of the wild type. The wide rrd2 root primordia develop into fasciated lateral roots. The stele of fasciated roots contains more cell files than the stele of normal roots. At 22°C on RIM, rrd2 and wild type root explants produce lateral roots of very similar morphology. When grown at 28°C for 16 days, rrd2 plantlets have shorter primary roots and less true leaves than the wild type. At 22°C, rrd2 plantlets look like the wild type (Sugiyama, 2003, Plant Cell Physiol. 44: 588–596; Otsuka and Sugiyama, 2012, J Plant Res. 125: 547-554).
Control Ler
Arabidopsis accession: Landsberg erecta.

16°C (det3) / untreated etiolated seedlings (det3)

Relative Expression (log2-ratio):-1.8042612
Number of Samples:2 / 2
Experimental 16°C (det3)
Four-day-old etiolated det3 seedling samples, grown on PA medium (0mM KNO3 added) at 16°C.
Control untreated etiolated seedlings (det3)
Four-day-old etiolated det3 seedling samples, grown on PA medium (0mM KNO3 added) at 22°C.

germination (48h) / seed desiccation

Relative Expression (log2-ratio):-1.7907343
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (48h) / stratification (48h)

Relative Expression (log2-ratio):-1.7485933
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.