TOP TEN perturbations for AT5G61030 (Arabidopsis thaliana)
Organism: Arabidopsis thaliana
Gene: AT5G61030
Selected probe(set): 247575_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array
Expression of AT5G61030 (247575_at) across 3240 perturbations tested by GENEVESTIGATOR:
24-eBL + glucose (dark) / 24-eBL (dark)
Relative Expression (log2-ratio):3.625206Number of Samples:3 / 3
| Experimental | 24-eBL + glucose (dark) |
| Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 0.1µM 24-epibrassinolide, 3% glucose, and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm. | |
| Control | 24-eBL (dark) |
| Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 0.1µM 24-epibrassinolide and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm. |
shift low to high light study 4 (Col-0) / low light study 5 (Col-0)
Relative Expression (log2-ratio):3.5681334Number of Samples:3 / 3
| Experimental | shift low to high light study 4 (Col-0) |
| Rosette samples of Col-0 plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days, and then shifted to high light (800µmol m-2 s-1) for 6h. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1). | |
| Control | low light study 5 (Col-0) |
| Rosette samples of Col-0 plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days and 6h. For the last 6h the plants were kept in light. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1). |
shift low to high light study 4 (rap2.4a) / low light study 5 (rap2.4a)
Relative Expression (log2-ratio):3.453312Number of Samples:3 / 3
| Experimental | shift low to high light study 4 (rap2.4a) |
| Rosette samples of rap2.4a plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days, and then shifted to high light (800µmol m-2 s-1) for 6h. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1). | |
| Control | low light study 5 (rap2.4a) |
| Rosette samples of rap2.4a plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days and 6h. For the last 6h the plants were kept in light. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1). |
glucose study 2 (dark) / mock treated seedling samples
Relative Expression (log2-ratio):3.4008684Number of Samples:2 / 3
| Experimental | glucose study 2 (dark) |
| Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 3% glucose and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm. | |
| Control | mock treated seedling samples |
| Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), and incubated for 27h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm. |
callus formation study 3 (35d + 1d) / untreated hypocotyl samples (35d)
Relative Expression (log2-ratio):3.390339Number of Samples:3 / 3
| Experimental | callus formation study 3 (35d + 1d) |
| Hypocotyl segments (0.5cm just above the root-hypocotyl junction) were cut out from 35-days-old Col-0 plants, incubated for 1 day on solid callus inducing medium (Murashige and Skoog salts, 2.0% (w/v) glucose, 0.5 p.p.m. (w/v) 2,4-D (2,4-dichlorophenoxyacetic acid), 0.05% (w/v) MES, 0.25% (w/v) gellan gum; pH 5.7) in darkness, and harvested. Plant growth conditions prior the treatment: vertically oriented plates with solid germination medium (Murashige and Skoog salts, 1.0% (w/v) sucrose, 0.05% (w/v) MES, 1.5% (w/v) agar; pH 5,7); 16h light (4–6µmol photons m-2 s-1) / 8h dark cycles; 25°C. | |
| Control | untreated hypocotyl samples (35d) |
| Hypocotyl samples (0.5cm-long segments just above the root-hypocotyl junction) of 35-days-old Col-0 plants grown on vertically oriented plates with solid germination medium (Murashige and Skoog salts, 1.0% (w/v) sucrose, 0.05% (w/v) MES, 1.5% (w/v) agar; pH 5,7) under 16h light (4–6µmol photons m-2 s-1) / 8h dark cycles at 25°C. |
germination (1h) / seed desiccation
Relative Expression (log2-ratio):3.3891068Number of Samples:2 / 3
| Experimental | germination (1h) |
| Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 1h. | |
| Control | seed desiccation |
| Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness. |
24-eBL + glucose (dark) / mock treated seedling samples
Relative Expression (log2-ratio):3.284809Number of Samples:3 / 3
| Experimental | 24-eBL + glucose (dark) |
| Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 0.1µM 24-epibrassinolide, 3% glucose, and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm. | |
| Control | mock treated seedling samples |
| Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), and incubated for 27h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm. |
cold study 21 (Col) / untreated rosette leaf samples (Col)
Relative Expression (log2-ratio):3.1706676Number of Samples:3 / 2
| Experimental | cold study 21 (Col) |
| Mature rosette leaf samples of Col grown for 4 weeks under 12h light (120μmol photons m-2 s-1) at 22°C / 12h dark at 18°C cycles, then, at the beginning of the light period, transferred to 7°C (12h light (120μmol photons m-2 s-1) / 12h dark) for 2 days. Other conditions: plants were grown on a mixture of vermiculite, perlite, and soil (1:1:1), fertilized with half strength Hoagland solution once per week. | |
| Control | untreated rosette leaf samples (Col) |
| Mature rosette leaf samples of Col grown for 4 weeks under 12h light (120μmol photons m-2 s-1) at 22°C / 12h dark at 18°C cycles. Other conditions: plants were grown on a mixture of vermiculite, perlite, and soil (1:1:1), fertilized with half strength Hoagland solution once per week. |
stratification (48h) / seed desiccation
Relative Expression (log2-ratio):3.1605873Number of Samples:3 / 3
| Experimental | stratification (48h) |
| Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h. | |
| Control | seed desiccation |
| Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness. |
germination (6h) / seed desiccation
Relative Expression (log2-ratio):3.1528597Number of Samples:3 / 3
| Experimental | germination (6h) |
| Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 6h. | |
| Control | seed desiccation |
| Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness. |