TOP TEN perturbations for AT5G61030 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: AT5G61030
Selected probe(set): 247575_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of AT5G61030 (247575_at) across 3240 perturbations tested by GENEVESTIGATOR:

24-eBL + glucose (dark) / 24-eBL (dark)

Relative Expression (log2-ratio):3.625206
Number of Samples:3 / 3
Experimental 24-eBL + glucose (dark)
Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 0.1µM 24-epibrassinolide, 3% glucose, and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm.
Control 24-eBL (dark)
Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 0.1µM 24-epibrassinolide and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm.

shift low to high light study 4 (Col-0) / low light study 5 (Col-0)

Relative Expression (log2-ratio):3.5681334
Number of Samples:3 / 3
Experimental shift low to high light study 4 (Col-0)
Rosette samples of Col-0 plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days, and then shifted to high light (800µmol m-2 s-1) for 6h. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).
Control low light study 5 (Col-0)
Rosette samples of Col-0 plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days and 6h. For the last 6h the plants were kept in light. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).

shift low to high light study 4 (rap2.4a) / low light study 5 (rap2.4a)

Relative Expression (log2-ratio):3.453312
Number of Samples:3 / 3
Experimental shift low to high light study 4 (rap2.4a)
Rosette samples of rap2.4a plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days, and then shifted to high light (800µmol m-2 s-1) for 6h. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).
Control low light study 5 (rap2.4a)
Rosette samples of rap2.4a plants grown on soil for 21 day under short day standard intensity light conditions (10h light (100µmol m-2 s-1) at 23°C / 14h dark at 18°C cycles), then shifted to low light (10h (8µmol m-2 s-1) at 23°C / 14h dark at 18°C) for 10 days and 6h. For the last 6h the plants were kept in light. Other conditions: 55% relative humidity; soil mixture: Fruhsdorfer Erde Klocke P, perlite, and vermiculite (1:1:1).

glucose study 2 (dark) / mock treated seedling samples

Relative Expression (log2-ratio):3.4008684
Number of Samples:2 / 3
Experimental glucose study 2 (dark)
Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 3% glucose and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm.
Control mock treated seedling samples
Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), and incubated for 27h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm.

callus formation study 3 (35d + 1d) / untreated hypocotyl samples (35d)

Relative Expression (log2-ratio):3.390339
Number of Samples:3 / 3
Experimental callus formation study 3 (35d + 1d)
Hypocotyl segments (0.5cm just above the root-hypocotyl junction) were cut out from 35-days-old Col-0 plants, incubated for 1 day on solid callus inducing medium (Murashige and Skoog salts, 2.0% (w/v) glucose, 0.5 p.p.m. (w/v) 2,4-D (2,4-dichlorophenoxyacetic acid), 0.05% (w/v) MES, 0.25% (w/v) gellan gum; pH 5.7) in darkness, and harvested. Plant growth conditions prior the treatment: vertically oriented plates with solid germination medium (Murashige and Skoog salts, 1.0% (w/v) sucrose, 0.05% (w/v) MES, 1.5% (w/v) agar; pH 5,7); 16h light (4–6µmol photons m-2 s-1) / 8h dark cycles; 25°C.
Control untreated hypocotyl samples (35d)
Hypocotyl samples (0.5cm-long segments just above the root-hypocotyl junction) of 35-days-old Col-0 plants grown on vertically oriented plates with solid germination medium (Murashige and Skoog salts, 1.0% (w/v) sucrose, 0.05% (w/v) MES, 1.5% (w/v) agar; pH 5,7) under 16h light (4–6µmol photons m-2 s-1) / 8h dark cycles at 25°C.

germination (1h) / seed desiccation

Relative Expression (log2-ratio):3.3891068
Number of Samples:2 / 3
Experimental germination (1h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 1h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

24-eBL + glucose (dark) / mock treated seedling samples

Relative Expression (log2-ratio):3.284809
Number of Samples:3 / 3
Experimental 24-eBL + glucose (dark)
Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), incubated for 24h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm, and then incubated in liquid medium containing 0.1µM 24-epibrassinolide, 3% glucose, and 0.5x MS salts for 3h in darkness at 22°C with shaking at 140rpm.
Control mock treated seedling samples
Seedling samples of Col grown for 5 days on solid medium (0.5x Murashige and Skoog (MS) salts, 1% sucrose, 0.8% agar) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 22°C, then washed seven times with water and once with liquid medium (0.5x MS salts), and incubated for 27h in liquid medium (0.5x MS salts) in darkness at 22°C with shaking at 140rpm.

cold study 21 (Col) / untreated rosette leaf samples (Col)

Relative Expression (log2-ratio):3.1706676
Number of Samples:3 / 2
Experimental cold study 21 (Col)
Mature rosette leaf samples of Col grown for 4 weeks under 12h light (120μmol photons m-2 s-1) at 22°C / 12h dark at 18°C cycles, then, at the beginning of the light period, transferred to 7°C (12h light (120μmol photons m-2 s-1) / 12h dark) for 2 days. Other conditions: plants were grown on a mixture of vermiculite, perlite, and soil (1:1:1), fertilized with half strength Hoagland solution once per week.
Control untreated rosette leaf samples (Col)
Mature rosette leaf samples of Col grown for 4 weeks under 12h light (120μmol photons m-2 s-1) at 22°C / 12h dark at 18°C cycles. Other conditions: plants were grown on a mixture of vermiculite, perlite, and soil (1:1:1), fertilized with half strength Hoagland solution once per week.

stratification (48h) / seed desiccation

Relative Expression (log2-ratio):3.1605873
Number of Samples:3 / 3
Experimental stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (6h) / seed desiccation

Relative Expression (log2-ratio):3.1528597
Number of Samples:3 / 3
Experimental germination (6h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 6h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.