TOP TEN perturbations for At1g71695 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: At1g71695
Selected probe(set): 261518_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of At1g71695 (261518_at) across 3240 perturbations tested by GENEVESTIGATOR:

cycloheximide + estradiol (35S:SND1-HER) / cycloheximide study 3 (35S:SND1-HER)

Relative Expression (log2-ratio):3.9849024
Number of Samples:2 / 2
Experimental cycloheximide + estradiol (35S:SND1-HER)
Samples of Arabidopsis leaf protoplasts transfected with the CaMV35S:SND1-HER-RR:NOS (35S:SND1-HER; in the pBI221 vector) construct and then treated with 2μM cycloheximide (an inhibitor of protein synthesis) and 2μM β-estradiol for 6h.
Control cycloheximide study 3 (35S:SND1-HER)
Samples of Arabidopsis leaf protoplasts transfected with the CaMV35S:SND1-HER-RR:NOS (35S:SND1-HER; in the pBI221 vector) construct and then treated with 2μM cycloheximide (an inhibitor of protein synthesis) for 6h.

callus formation study 3 (7d + 1d) / untreated hypocotyl samples (7d)

Relative Expression (log2-ratio):-3.922388
Number of Samples:3 / 3
Experimental callus formation study 3 (7d + 1d)
Hypocotyl segments (0.5cm just above the root-hypocotyl junction) were cut out from 7-days-old Col-0 seedlings, incubated for 1 day on solid callus inducing medium (Murashige and Skoog salts, 2.0% (w/v) glucose, 0.5 p.p.m. (w/v) 2,4-D (2,4-dichlorophenoxyacetic acid), 0.05% (w/v) MES, 0.25% (w/v) gellan gum; pH 5.7) in darkness, and harvested. Plant growth conditions prior the treatment: vertically oriented plates with solid germination medium (Murashige and Skoog salts, 1.0% (w/v) sucrose, 0.05% (w/v) MES, 1.5% (w/v) agar; pH 5,7); 16h light (4–6µmol photons m-2 s-1) / 8h dark cycles; 25°C.
Control untreated hypocotyl samples (7d)
Hypocotyl samples (0.5cm-long segments just above the root-hypocotyl junction) of 7-days-old Col-0 seedlings grown on vertically oriented plates with solid germination medium (Murashige and Skoog salts, 1.0% (w/v) sucrose, 0.05% (w/v) MES, 1.5% (w/v) agar; pH 5,7) under 16h light (4–6µmol photons m-2 s-1) / 8h dark cycles at 25°C.

light/drought (aox1a(sail)) / untreated leaf samples (aox1a(sail))

Relative Expression (log2-ratio):-3.8477116
Number of Samples:2 / 2
Experimental light/drought (aox1a(sail))
4-week-old plants, carrying a T-DNA insertion for AOX1a (SAIL_030_D08), grown under normal conditions with watering every 2 days, transferred to moderate light conditions (approximately 250 µE m-2 s-1) with no water (starting 4 d prior to transfer to ensure that soil was dry), for 3 days.
Control untreated leaf samples (aox1a(sail))
4-week-old plants, carrying a T-DNA insertion for AOX1a (SAIL_030_D08) grown under normal conditions with watering every 2 days.

cycloheximide + estradiol (35S:VND7-HER) / cycloheximide study 3 (35S:VND7-HER)

Relative Expression (log2-ratio):3.7592916
Number of Samples:2 / 2
Experimental cycloheximide + estradiol (35S:VND7-HER)
Samples of Arabidopsis leaf protoplasts transfected with the CaMV35S:VND7-HER-RR:NOS (35S:VND7-HER; in the pBI221 vector) construct and then treated with 2μM cycloheximide (an inhibitor of protein synthesis) and 2μM β-estradiol for 6h.
Control cycloheximide study 3 (35S:VND7-HER)
Samples of Arabidopsis leaf protoplasts transfected with the CaMV35S:VND7-HER-RR:NOS (35S:VND7-HER; in the pBI221 vector) construct and then treated with 2μM cycloheximide (an inhibitor of protein synthesis) for 6h.

mannitol study 2 (rgs1-2) / mock treated rgs1-2 guard cell samples

Relative Expression (log2-ratio):-3.7538452
Number of Samples:3 / 3
Experimental mannitol study 2 (rgs1-2)
Guard cell pairs were manually dissected from rgs1-2 mature leaf blades that had been cut into 1mm wide strips, floated on a solution containing 150mM mannitol, 50mM KCl, 0.1mM CaCl2, 10mM MES-NaOH (pH 6.1), treated with vacuum (25 KPa) for 30s, incubated for 5h (22°C, 30μmol photons m-2 s-1) in the solution mentioned above, then blotted dry, frozen in liquid N2, freeze-dried for 4 days under vacuum of < 10mm of mercury, and stored under vacuum at 20°C. Before cutting the leaves, plants were grown for 8-10 weeks at 22°C, 60% relative humidity, under 8h light (150μmol photons m-2 s-1) / 16h dark cycles on Superfine Germinating Mix (FaFard).
Control mock treated rgs1-2 guard cell samples
Guard cell pairs were manually dissected from rgs1-2 mature leaf blades that had been cut into 1mm wide strips, floated on a solution containing 50mM KCl, 0.1mM CaCl2, 10mM MES-NaOH (pH 6.1), treated with vacuum (25 KPa) for 30s, incubated for 5h (22°C, 30μmol photons m-2 s-1) in the solution mentioned above, then blotted dry, frozen in liquid N2, freeze-dried for 4 days under vacuum of < 10mm of mercury, and stored under vacuum at 20°C. Before cutting the leaves, plants were grown for 8-10 weeks at 22°C, 60% relative humidity, under 8h light (150μmol photons m-2 s-1) / 16h dark cycles on Superfine Germinating Mix (FaFard).

brxS / Sav-0

Relative Expression (log2-ratio):-3.6292477
Number of Samples:2 / 2
Experimental brxS
Loss-of-function brx mutation is a natural mutation found in Arabidopsis accession Uk-1. The BRX (BREVIS RADIX, At1g31880) gene of Uk-1 carries a stop codon in the 4th (2nd translated) exon. The mutation has been introduced into Sav-0 genetic background giving rise to the brxS mutant line. BRX belongs to the five-member BRX gene family and controls the extent of cell proliferation and elongation in the growth zone of the root tip. Uk-1 and brxS seedlings develop shorter primary root than Sav-0 wild type. Root system of mature Uk-1 and brxS plants is shorter than that of Sav-0 wild type. brxS roots are composed of shorter and fewer cells than Sav-0 roots. Root apical meristem is smaller in brxS seedllings compared to Sav-0 (Mouchel et al., 2004, Genes Dev. 18: 700-714).
Control Sav-0
Arabidopsis ecotype from Slavice (Czechoslovakia); NASC ID: N1515

imidacloprid (4d) / mock treated Col-0 rosette leaf samples (4d)

Relative Expression (log2-ratio):-3.3575726
Number of Samples:2 / 2
Experimental imidacloprid (4d)
Rosette leaf samples of Col-0 grown for 4 weeks on soil (12h light (100µmol photons m-2 s-1) / 12h darkness), then treated with 4mM imidacloprid via soil application, and kept for 4d. Imidacloprid is a systemic insecticide from the neonicotinoid class of insecticides.
Control mock treated Col-0 rosette leaf samples (4d)
Rosette leaf samples of Col-0 grown for 4 weeks on soil (12h light (100µmol photons m-2 s-1) / 12h darkness), then watered via soil application, and kept for 4d.

brxS / Sav-0

Relative Expression (log2-ratio):-3.333559
Number of Samples:2 / 2
Experimental brxS
Loss-of-function brx mutation is a natural mutation found in Arabidopsis accession Uk-1. The BRX (BREVIS RADIX, At1g31880) gene of Uk-1 carries a stop codon in the 4th (2nd translated) exon. The mutation has been introduced into Sav-0 genetic background giving rise to the brxS mutant line. BRX belongs to the five-member BRX gene family and controls the extent of cell proliferation and elongation in the growth zone of the root tip. Uk-1 and brxS seedlings develop shorter primary root than Sav-0 wild type. Root system of mature Uk-1 and brxS plants is shorter than that of Sav-0 wild type. brxS roots are composed of shorter and fewer cells than Sav-0 roots. Root apical meristem is smaller in brxS seedllings compared to Sav-0 (Mouchel et al., 2004, Genes Dev. 18: 700-714).
Control Sav-0
Arabidopsis ecotype from Slavice (Czechoslovakia); NASC ID: N1515

callus formation (96h) / untreated root samples

Relative Expression (log2-ratio):3.2964735
Number of Samples:3 / 3
Experimental callus formation (96h)
Root segments were cut out from 10-days-old Col-0 plantlets and incubated on callus inducing medium for 96h. Plant growth conditions prior the treatment: solid Murashige and Skoog medium, 22°C, 16h light / 8h dark.
Control untreated root samples
Root segments were cut out from 10-days-old Col-0 plantlets grown on solid Murashige and Skoog medium, 22°C, 16h light / 8h dark.

long day (Col-0) / short day study 2 (Col-0)

Relative Expression (log2-ratio):3.2188244
Number of Samples:3 / 3
Experimental long day (Col-0)
Leaf samples of Col-0 grown on soil (supplemented with Hoagland medium) under long day conditions (16h light (120 -160μmol photons m-2 s-1) at 20°C / 8h darkness at 18°C) for 21 day.
Control short day study 2 (Col-0)
Leaf samples of Col-0 grown on soil (supplemented with Hoagland medium) under short day conditions (8h light (140 -160μmol photons m-2 s-1) at 20°C / 16h darkness at 18°C) for 35 days.