TOP TEN perturbations for O22969 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: O22969
Selected probe(set): 256723_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of O22969 (256723_at) across 3240 perturbations tested by GENEVESTIGATOR:

ABA study 7 (gpa1-4) / solvent treated guard cell samples (gpa1-4)

Relative Expression (log2-ratio):-2.9440584
Number of Samples:3 / 3
Experimental ABA study 7 (gpa1-4)
Isolated guard cell samples of 5 weeks old Arabidopsis thaliana gpa1-4 T-DNA insertion mutant plants, treated with 50mM ABA for 3h.
Control solvent treated guard cell samples (gpa1-4)
Guard cell samples of 5 weeks old Arabidopsis thaliana gpa1-4 T-DNA insertion mutant plants, treated with EtOH for 3h.

ABA study 7 (agb1-2 gpa1-4) / solvent treated guard cell samples (agb1-2 gpa1-4)

Relative Expression (log2-ratio):-2.6018686
Number of Samples:2 / 3
Experimental ABA study 7 (agb1-2 gpa1-4)
Isolated guard cell samples of 5 weeks old Arabidopsis thaliana agb1-2 gpa1-4 T-DNA insertion double mutant plants, treated with 50mM ABA for 3h.
Control solvent treated guard cell samples (agb1-2 gpa1-4)
Guard cell samples of 5 weeks old Arabidopsis thaliana agb1-2 gpa1-4 T-DNA insertion double mutant plants, treated with EtOH for 3h.

ABA study 7 (agb1-2) / solvent treated guard cell samples (agb1-2)

Relative Expression (log2-ratio):-2.248207
Number of Samples:3 / 3
Experimental ABA study 7 (agb1-2)
Isolated guard cell samples of 5 weeks old Arabidopsis thaliana agb1-2 T-DNA insertion mutant plants, treated with 50mM ABA for 3h.
Control solvent treated guard cell samples (agb1-2)
Guard cell samples of 5 weeks old Arabidopsis thaliana agb1-2 T-DNA insertion mutant plants, treated with EtOH for 3h.

IVT-E RNA labeling (root elongation zone) / two-cycle RNA labeling (root elongation zone)

Relative Expression (log2-ratio):2.1849222
Number of Samples:3 / 3
Experimental IVT-E RNA labeling (root elongation zone)
Root elongation zone samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix GeneChip 3' IVT-Express Kit.
Control two-cycle RNA labeling (root elongation zone)
Root elongation zone samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix Two-Cycle Eukaryotic Target Labeling Assay kit.

ABA study 7 (Col-0) / solvent treated guard cell samples (Col-0)

Relative Expression (log2-ratio):-2.1453009
Number of Samples:3 / 3
Experimental ABA study 7 (Col-0)
Isolated guard cell samples of 5 weeks old Arabidopsis thaliana Col-0 wild-type plants, treated with 50mM ABA for 3h.
Control solvent treated guard cell samples (Col-0)
Guard cell samples of 5 weeks old Arabidopsis thaliana Col-0 wild-type plants, treated with EtOH for 3h.

IVT-E RNA labeling (root tip) / two-cycle RNA labeling (root tip)

Relative Expression (log2-ratio):2.050724
Number of Samples:3 / 3
Experimental IVT-E RNA labeling (root tip)
Root tip samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix GeneChip 3' IVT-Express Kit.
Control two-cycle RNA labeling (root tip)
Root tip samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix Two-Cycle Eukaryotic Target Labeling Assay kit.

two-cycle RNA labeling (root elongation zone) / one-cycle RNA labeling (root elongation zone)

Relative Expression (log2-ratio):-1.9250221
Number of Samples:3 / 3
Experimental two-cycle RNA labeling (root elongation zone)
Root elongation zone samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix Two-Cycle Eukaryotic Target Labeling Assay kit.
Control one-cycle RNA labeling (root elongation zone)
Root elongation zone samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix One-Cycle Eukaryotic Target Labeling Assay kit.

two-cycle RNA labeling (root tip) / one-cycle RNA labeling (root tip)

Relative Expression (log2-ratio):-1.8842592
Number of Samples:3 / 3
Experimental two-cycle RNA labeling (root tip)
Root tip samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix Two-Cycle Eukaryotic Target Labeling Assay kit.
Control one-cycle RNA labeling (root tip)
Root tip samples of Col-0 grown for 7 days on vertically oriented plates containing solid 0.5x Murashige and Skoog medium under continuous light (150µmol photons m-2 s-1) at 24°C. For microarrays RNA was labeled using Affymetrix One-Cycle Eukaryotic Target Labeling Assay kit.

oxt6 / Col-0

Relative Expression (log2-ratio):1.869112
Number of Samples:3 / 3
Experimental oxt6
The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit.
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

tbf1 / Col-0

Relative Expression (log2-ratio):1.784893
Number of Samples:3 / 3
Experimental tbf1
Knockout mutant carrying T-DNA insertion (SALK_104713) in the intron of TBF1 (TL1-binding factor; At4g36990). TBF1 is a heat-shock factor-like transcription factor that specifically binds to the TL1 (GAAGAAGAA) cis element enriched in promoters of genes regulated by microbe-associated molecular patterns (e.g. EF-Tu(elf26), flg22) and in promoters of some salicylic acid (SA)-induced genes encoding endoplasmic reticulum-localized proteins. In the presence of 150-250µM SA or 10µM EF-Tu(elf18), growth of tbf1 seedlings is less inhibited than growth of Col-0 wild type. Rosette stage wild type plants pre-treated with SA or EF-Tu(elf18) become more resistant to bacterial pathogen Pseudomonas syringae pv. maculicola ES4326 whereas tbf1 plants pre-treated the same way remain almost as susceptible to the pathogen as the mock-treated tbf1 and wild type plants. Flg22-induced resistance to P. syringae pv. maculicola ES4326 is not compromised in tbf1. Compared to the wild type, tbf1 is more susceptible to tunicamycin, an inducer of unfolded protein response (Pajerowska-Mukhtar et al., 2012, Curr Biol. 22: 103-112).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).