TOP TEN perturbations for O23365 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: O23365
Selected probe(set): 245532_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of O23365 (245532_at) across 3240 perturbations tested by GENEVESTIGATOR:

35:LOB-GR / Col-0

Relative Expression (log2-ratio):2.8238525
Number of Samples:3 / 3
Experimental 35:LOB-GR
Transgenic line expressing the LOB-GR fusion protein from the constitutive CaMV35S promoter. LOB (LATERAL ORGAN BOUNDARIES, encoded by At5g63090) is a plant-specific transcription factor regulating accumulation of brassinosteroids in organ boundaries; GR is the steroid-binding domain (amino acids 512 to 795) of the rat glucocorticoid receptor. GR is fused in frame to the C-terminus of the full-length LOB protein. In the absence of dexamethasone (DEX), a synthetic glucocorticoid, the LOB-GR fusion protein is sequestered in the cytosol via binding to HSP90 and thus inactive. Binding of dexamethasone to GR releases LOB-GR, which can then enter the nucleus and regulate expression of the LOB target genes. When grown on 3µM DEX in light, 35:LOB-GR plants are dwarf and sterile; in the absence of DEX, 35:LOB-GR plants are like Col-0 wild type. Dark-grown 35S:LOB-GR seedlings have an apical hook when grown in the absence of DEX and lack an apical hook when grown on 3μM DEX. 35:LOB-GR seedlings grown in darkness on 3μM DEX are less sensitive to exogenous epi-brassinolide than 35:LOB-GR seedlings grown in darkness in the absence of DEX and dark-grown Col-0 wild type (Bell et al., 2012, PNAS 109: 21146-21151).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

brxS / Sav-0

Relative Expression (log2-ratio):-2.7968655
Number of Samples:2 / 2
Experimental brxS
Loss-of-function brx mutation is a natural mutation found in Arabidopsis accession Uk-1. The BRX (BREVIS RADIX, At1g31880) gene of Uk-1 carries a stop codon in the 4th (2nd translated) exon. The mutation has been introduced into Sav-0 genetic background giving rise to the brxS mutant line. BRX belongs to the five-member BRX gene family and controls the extent of cell proliferation and elongation in the growth zone of the root tip. Uk-1 and brxS seedlings develop shorter primary root than Sav-0 wild type. Root system of mature Uk-1 and brxS plants is shorter than that of Sav-0 wild type. brxS roots are composed of shorter and fewer cells than Sav-0 roots. Root apical meristem is smaller in brxS seedllings compared to Sav-0 (Mouchel et al., 2004, Genes Dev. 18: 700-714).
Control Sav-0
Arabidopsis ecotype from Slavice (Czechoslovakia); NASC ID: N1515

arr10-5:arr12-1 / Col-0

Relative Expression (log2-ratio):2.765977
Number of Samples:3 / 3
Experimental arr10-5:arr12-1
arr10 arr12 double mutant that exhibits a characteristic phenotype with regard to the cytokinin-mediated His-Asp phosphorelay.
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

35:LOB-GR / Col-0

Relative Expression (log2-ratio):2.7421694
Number of Samples:3 / 3
Experimental 35:LOB-GR
Transgenic line expressing the LOB-GR fusion protein from the constitutive CaMV35S promoter. LOB (LATERAL ORGAN BOUNDARIES, encoded by At5g63090) is a plant-specific transcription factor regulating accumulation of brassinosteroids in organ boundaries; GR is the steroid-binding domain (amino acids 512 to 795) of the rat glucocorticoid receptor. GR is fused in frame to the C-terminus of the full-length LOB protein. In the absence of dexamethasone (DEX), a synthetic glucocorticoid, the LOB-GR fusion protein is sequestered in the cytosol via binding to HSP90 and thus inactive. Binding of dexamethasone to GR releases LOB-GR, which can then enter the nucleus and regulate expression of the LOB target genes. When grown on 3µM DEX in light, 35:LOB-GR plants are dwarf and sterile; in the absence of DEX, 35:LOB-GR plants are like Col-0 wild type. Dark-grown 35S:LOB-GR seedlings have an apical hook when grown in the absence of DEX and lack an apical hook when grown on 3μM DEX. 35:LOB-GR seedlings grown in darkness on 3μM DEX are less sensitive to exogenous epi-brassinolide than 35:LOB-GR seedlings grown in darkness in the absence of DEX and dark-grown Col-0 wild type (Bell et al., 2012, PNAS 109: 21146-21151).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).

brxS / Sav-0

Relative Expression (log2-ratio):-2.6159248
Number of Samples:2 / 2
Experimental brxS
Loss-of-function brx mutation is a natural mutation found in Arabidopsis accession Uk-1. The BRX (BREVIS RADIX, At1g31880) gene of Uk-1 carries a stop codon in the 4th (2nd translated) exon. The mutation has been introduced into Sav-0 genetic background giving rise to the brxS mutant line. BRX belongs to the five-member BRX gene family and controls the extent of cell proliferation and elongation in the growth zone of the root tip. Uk-1 and brxS seedlings develop shorter primary root than Sav-0 wild type. Root system of mature Uk-1 and brxS plants is shorter than that of Sav-0 wild type. brxS roots are composed of shorter and fewer cells than Sav-0 roots. Root apical meristem is smaller in brxS seedllings compared to Sav-0 (Mouchel et al., 2004, Genes Dev. 18: 700-714).
Control Sav-0
Arabidopsis ecotype from Slavice (Czechoslovakia); NASC ID: N1515

BL study 2 (brx) / mock treated seedlings (brx)

Relative Expression (log2-ratio):2.5907612
Number of Samples:2 / 2
Experimental BL study 2 (brx)
At 4 days after germination in liquid media, brx seedlings were treated with 5nM brassinolide for 3 hours.
Control mock treated seedlings (brx)
At 4 days after germination in liquid media, brx seedlings were mock-treated.

callus formation study 2 (96h) / untreated shoot samples

Relative Expression (log2-ratio):-2.522646
Number of Samples:3 / 3
Experimental callus formation study 2 (96h)
Shoot segments were cut out from 10-days-old Col-0 plantlets and incubated on callus inducing medium for 96h. Plant growth conditions prior the treatment: solid Murashige and Skoog medium, 22°C, 16h light / 8h dark.
Control untreated shoot samples
Shoot segments were cut out from 10-days-old Col-0 plantlets grown on solid Murashige and Skoog medium, 22°C, 16h light / 8h dark.

35S-PaWRKY1 / empty-pCAMBIA1380

Relative Expression (log2-ratio):2.3680344
Number of Samples:3 / 3
Experimental 35S-PaWRKY1
Transgenic line (Col genetic background) expressing Phalaenopsis amabilis PaWRKY1 from the constitutive CaMV35S promoter (prior transformation of Arabidopsis, the coding region of PaWRKY1 cDNA was cloned into the binary vector pCAMBIA1380). PaWRKY1 is homologous to Arabidopsis transcription factor AtWRKY75 (Fu et al., 2012, Physiol Plant. 145: 406-425).
Control empty-pCAMBIA1380
Transgenic line obtained by transforming Arabidopsis (Col) with empty binary vector pCAMBIA1380 (Fu et al., 2012, Physiol Plant. 145: 406-425).

shift 28°C to 19°C study 3 (35S:RPS4-HS) / 28°C (35S:RPS4-HS)

Relative Expression (log2-ratio):-2.2202797
Number of Samples:3 / 3
Experimental shift 28°C to 19°C study 3 (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C and then shifted to 19°C for 24h. Other conditions: growth chamber, 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.
Control 28°C (35S:RPS4-HS)
Leaf samples of 35S:RPS4-HS plants grown for 3.5 weeks on soil at 28°C under 10h light (150-200µmol photons m-2 s-1) / 14h dark cycles, 65% relative humidity.

ARR21Cox / Col-0

Relative Expression (log2-ratio):-2.1822128
Number of Samples:3 / 3
Experimental ARR21Cox
Transgenic plants where the cDNA encoding the C-terminal DNA-binding domain of ARR21 was designed so as to be overexpressed under the control of the cauliflower mosaic virus (CaMV) 35S promoter.
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816). In the study by (Nakano et al., 2020, Front Plant Sci. 11: 405), aluminum tolerance of Col-0 was 53.3; calculated as: (root length of 5-day-old seedlings grown in the presence of 5µM AlCl3 at pH 5.0 / root length of 5-day-old seedlings grown without Al at pH 5.0) x 100. Col-0 is moderately tolerant to submergence. For example, when rosette-stage Col-0 plants were completely submerged in water for 2 days under short-day conditions, the photosynthetic rate in their leaves decreased by approx. 36.4% compared to untreated Col-0 (Meng et al., 2020, Plant J. 103: 227-247).