TOP TEN perturbations for O23561 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: O23561
Selected probe(set): 245406_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of O23561 (245406_at) across 3240 perturbations tested by GENEVESTIGATOR:

syringolin study 2 / solvent treated leaf samples (syl_404_bc2)

Relative Expression (log2-ratio):2.3346395
Number of Samples:2 / 2
Experimental syringolin study 2
Arabidopsis mutant plants (syl_404_bc2) were sprayed with 20 uM syringolin A. Hybridization probes were prepared from primary leaves harvested 8 h after syringolin treatment.
Control solvent treated leaf samples (syl_404_bc2)
Arabidopsis mutant plants (syl_404_bc2) were sprayed with a control buffer (0.05% Tween 20). Hybridization probes were prepared from primary leaves harvested 8 h after treatment.

syringolin study 3 (late) / solvent treated leaf samples (Col-0; late)

Relative Expression (log2-ratio):2.1044397
Number of Samples:3 / 3
Experimental syringolin study 3 (late)
Primary leaf samples of uninfected Col-0 plants, treated with Syringolin A (sylA) solution (20 uM) for 8h to 12h.
Control solvent treated leaf samples (Col-0; late)
Primary leaf samples of uninfected Col-0 plants, treated with control buffer solution (Tween 20 0.05%) for 8h to 12h.

germination (24h) / seed desiccation

Relative Expression (log2-ratio):-1.3127279
Number of Samples:3 / 3
Experimental germination (24h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 24h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (48h) / seed desiccation

Relative Expression (log2-ratio):-1.2045231
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (12h) / seed desiccation

Relative Expression (log2-ratio):-1.1875181
Number of Samples:3 / 3
Experimental germination (12h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 12h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

stratification (48h) / seed desiccation

Relative Expression (log2-ratio):-1.051445
Number of Samples:3 / 3
Experimental stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (6h) / seed desiccation

Relative Expression (log2-ratio):-1.0423622
Number of Samples:3 / 3
Experimental germination (6h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 6h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

germination (1h) / seed desiccation

Relative Expression (log2-ratio):-0.90787697
Number of Samples:2 / 3
Experimental germination (1h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 1h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

IAA / FACS (pWER::GFP) / root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP

Relative Expression (log2-ratio):0.81554794
Number of Samples:3 / 3
Experimental IAA / FACS (pWER::GFP)
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 7 days hydroponically in phytatrays with growth medium (0.5x Murashige and Skoog salts, 1% (w/v) sucrose, 0.5 g/l MES hydrate; pH 5.7), then transferred to 5μM IAA (indole-3-acetic acid) solution for 2h (total IAA treatment time was 3h as IAA was also present during the protoplast isolation and FACS sorting procedures). Other conditions: seedlings were grown in Advanced Intellus environmental controller (Percival), 18h light (35μmol photons m-2 s-1) / 6h dark, 22°C. FACS, Fluorescence Activated Cell Sorting.
Control root epidermis and lateral root cap protoplast samples of mock treated pWER::GFP
Root epidermis and lateral root cap protoplast samples were obtained by FACS-sorting of protoplasts isolated from whole roots of pWER::GFP seedlings that had been grown for 7 days hydroponically in phytatrays with growth medium (0.5x Murashige and Skoog salts, 1% (w/v) sucrose, 0.5 g/l MES hydrate; pH 5.7), then transferred to 0.05% (v/v) ethanol (solvent for IAA) for 2h (total mock-treatment time was 3h as ethanol was also present during the protoplast isolation and FACS sorting procedures). Other conditions: seedlings were grown in Advanced Intellus environmental controller (Percival), 18h light (35μmol photons m-2 s-1) / 6h dark, 22°C. FACS, Fluorescence Activated Cell Sorting.

dcl1-15 / DCL1 sib

Relative Expression (log2-ratio):0.80982685
Number of Samples:3 / 3
Experimental dcl1-15
EMS-generated mutant with leaky recessive missense mutation (Gly →Glu at amino acid position 1692) in DCL1 (At1g01040). DCL1 (RNase III DICER-LIKE1) is a part of a complex that cleaves precursor miRNA thus generating miRNAs. dcl1-15 embryos develop normally till the early globular stage, then the patterns of cell division become abnormal. dcl1-15 embryos mature faster than the wild type: dcl1-15 embryos start to accumulate chlorophyll already at the early globular stage and are visibly green at the heart stage whereas embryos of the wild type siblings are white at the heart stage (although they start accumulating chlorophyll in the protodermis at this stage); at the heart stage, chloroplasts of dcl1-15 embryos are more developed (more grana stacks per chloroplast, more thylakoids per grana stack) than those of the wild type. dcl1-15 embryos abort late in embryogenesis and this abortion is not due to desiccation intolerance (Willmann et al., 2011, Plant Physiol. 155: 1871-84).
Control DCL1 sib
Wild type sibling of dcl1-15 mutant. DCL1 sib was obtained by backcrossing heterozygous (dcl1-15 DCL1) plants (initially in the Ws genetic background) to Ler wild type at least four times (Willmann et al., 2011, Plant Physiol. 155: 1871-84).