TOP TEN perturbations for Q5CCK4 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: Q5CCK4
Selected probe(set): 253501_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of Q5CCK4 (253501_at) across 3243 perturbations tested by GENEVESTIGATOR:

tbf1 / Col-0

Relative Expression (log2-ratio):-2.094883
Number of Samples:3 / 3
Experimental tbf1
Knockout mutant carrying T-DNA insertion (SALK_104713) in the intron of TBF1 (TL1-binding factor; At4g36990). TBF1 is a heat-shock factor-like transcription factor that specifically binds to the TL1 (GAAGAAGAA) cis element enriched in promoters of genes regulated by microbe-associated molecular patterns (e.g. EF-Tu(elf26), flg22) and in promoters of some salicylic acid (SA)-induced genes encoding endoplasmic reticulum-localized proteins. In the presence of 150-250µM SA or 10µM EF-Tu(elf18), growth of tbf1 seedlings is less inhibited than growth of Col-0 wild type. Rosette stage wild type plants pre-treated with SA or EF-Tu(elf18) become more resistant to bacterial pathogen Pseudomonas syringae pv. maculicola ES4326 whereas tbf1 plants pre-treated the same way remain almost as susceptible to the pathogen as the mock-treated tbf1 and wild type plants. Flg22-induced resistance to P. syringae pv. maculicola ES4326 is not compromised in tbf1. Compared to the wild type, tbf1 is more susceptible to tunicamycin, an inducer of unfolded protein response (Pajerowska-Mukhtar et al., 2012, Curr Biol. 22: 103-112).
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816).

ABA study 8 (Col-0) / solvent treated leaf samples (Col-0)

Relative Expression (log2-ratio):-1.8860235
Number of Samples:3 / 3
Experimental ABA study 8 (Col-0)
Excised leaf samples of 5 weeks old Arabidopsis thaliana Col-0 wild-type plants, treated with 50mM ABA for 3h.
Control solvent treated leaf samples (Col-0)
Mature leaf samples of 5 weeks old Arabidopsis thaliana Col-0 wild-type plants, treated with EtOH for 3h.

16°C (det3) / untreated etiolated seedlings (det3)

Relative Expression (log2-ratio):-1.6879826
Number of Samples:2 / 2
Experimental 16°C (det3)
Four-day-old etiolated det3 seedling samples, grown on PA medium (0mM KNO3 added) at 16°C.
Control untreated etiolated seedlings (det3)
Four-day-old etiolated det3 seedling samples, grown on PA medium (0mM KNO3 added) at 22°C.

non-polysomal RNA study 2 (Col-0) / total RNA study 3 (Col-0)

Relative Expression (log2-ratio):-1.5878859
Number of Samples:4 / 3
Experimental non-polysomal RNA study 2 (Col-0)
Non-polysomal (monosomal and free) mRNA was isolated from shoots of 10-12 days old Col-0 seedlings grown on solid medium (1x Murashige and Skoog salts, 1% (w/v) sucrose; pH 5.7) under continuous light (80µmol photons m-2 s-1) at 22°C.
Control total RNA study 3 (Col-0)
Total RNA was isolated from shoots of 10-12 days old Col-0 seedlings grown on solid medium (1x Murashige and Skoog salts, 1% (w/v) sucrose; pH 5.7) under continuous light (80µmol photons m-2 s-1) at 22°C.

cycloheximide + estradiol (35S:VND7-HER) / cycloheximide study 3 (35S:VND7-HER)

Relative Expression (log2-ratio):1.4843845
Number of Samples:2 / 2
Experimental cycloheximide + estradiol (35S:VND7-HER)
Samples of Arabidopsis leaf protoplasts transfected with the CaMV35S:VND7-HER-RR:NOS (35S:VND7-HER; in the pBI221 vector) construct and then treated with 2μM cycloheximide (an inhibitor of protein synthesis) and 2μM β-estradiol for 6h.
Control cycloheximide study 3 (35S:VND7-HER)
Samples of Arabidopsis leaf protoplasts transfected with the CaMV35S:VND7-HER-RR:NOS (35S:VND7-HER; in the pBI221 vector) construct and then treated with 2μM cycloheximide (an inhibitor of protein synthesis) for 6h.

BL/H3BO3 (6d) / untreated cell culture samples

Relative Expression (log2-ratio):1.4604349
Number of Samples:2 / 2
Experimental BL/H3BO3 (6d)
Cells were transferred into medium that included 1 uM brassinolide and 10 mM H3BO3 for 6d.
Control untreated cell culture samples
No brassinolide applied (timepoint 0).

BL/H3BO3 (4d) / untreated cell culture samples

Relative Expression (log2-ratio):1.4122534
Number of Samples:2 / 2
Experimental BL/H3BO3 (4d)
Cells were transferred into medium that included 1 uM brassinolide and 10 mM H3BO3 for 4d.
Control untreated cell culture samples
No brassinolide applied (timepoint 0).

long day (Col-0) / short day study 2 (Col-0)

Relative Expression (log2-ratio):1.3041229
Number of Samples:3 / 3
Experimental long day (Col-0)
Leaf samples of Col-0 grown on soil (supplemented with Hoagland medium) under long day conditions (16h light (120 -160μmol photons m-2 s-1) at 20°C / 8h darkness at 18°C) for 21 day.
Control short day study 2 (Col-0)
Leaf samples of Col-0 grown on soil (supplemented with Hoagland medium) under short day conditions (8h light (140 -160μmol photons m-2 s-1) at 20°C / 16h darkness at 18°C) for 35 days.

cycloheximide / mock treated seedlings

Relative Expression (log2-ratio):-1.2601128
Number of Samples:2 / 2
Experimental cycloheximide
Cycloheximide (protein synthesis inhibitor); 10uM CHX for 3h.
Control mock treated seedlings
Mock treatment for 3h.

det3 / Col-0

Relative Expression (log2-ratio):-1.2529144
Number of Samples:2 / 2
Experimental det3
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816).