TOP TEN perturbations for Q949P1 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: Q949P1
Selected probe(set): 254562_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of Q949P1 (254562_at) across 3240 perturbations tested by GENEVESTIGATOR:

ABA + DMTU (20h) / solvent treated cell suspension samples (20h)

Relative Expression (log2-ratio):5.3930445
Number of Samples:3 / 3
Experimental ABA + DMTU (20h)
Cell suspension (cell line T87) was cultured in Gamborg B5 medium containing 3% sucrose and 0.5µM 1-naphthaleneacetic acid at 20°C under 16h light (24 μmol photons s−1 m−2) / 8h dark cycles, with shaking at 120rpm and subculturing every 7 days (10ml of the cell suspension were transferred to 90 ml of fresh medium). At the exponential phase of growth (approx. 120h after subculturing) ABA (abscisic acid) and DMTU (dimethylthiourea) were added to the final concentrations of 50μM (ABA) and 5mM (DMTU) and the cell suspension was cultured for another 20h prior harvesting the cells. DMTU is an H2O2 scavenger.
Control solvent treated cell suspension samples (20h)
Cell suspension (cell line T87) was cultured in Gamborg B5 medium containing 3% sucrose and 0.5µM 1-naphthaleneacetic acid at 20°C under 16h light (24 μmol photons s−1 m−2) / 8h dark cycles, with shaking at 120rpm and subculturing every 7 days (10ml of the cell suspension were transferred to 90 ml of fresh medium). At the exponential phase of growth (approx. 120h after subculturing) the cell suspension was treated with ethanol for 20h.

ABA study 10 (20h) / solvent treated cell suspension samples (20h)

Relative Expression (log2-ratio):5.1356134
Number of Samples:3 / 3
Experimental ABA study 10 (20h)
Cell suspension (cell line T87) was cultured in Gamborg B5 medium containing 3% sucrose and 0.5µM 1-naphthaleneacetic acid at 20°C under 16h light (24 μmol photons s−1 m−2) / 8h dark cycles, with shaking at 120rpm and subculturing every 7 days (10ml of the cell suspension were transferred to 90 ml of fresh medium). At the exponential phase of growth (approx. 120h after subculturing) ABA (abscisic acid) was added to the final concentration of 50μM and the cell suspension was cultured for another 20h prior harvesting the cells.
Control solvent treated cell suspension samples (20h)
Cell suspension (cell line T87) was cultured in Gamborg B5 medium containing 3% sucrose and 0.5µM 1-naphthaleneacetic acid at 20°C under 16h light (24 μmol photons s−1 m−2) / 8h dark cycles, with shaking at 120rpm and subculturing every 7 days (10ml of the cell suspension were transferred to 90 ml of fresh medium). At the exponential phase of growth (approx. 120h after subculturing) the cell suspension was treated with ethanol for 20h.

DFPM + ABA (Col-0) / solvent treated seedling samples (Col-0)

Relative Expression (log2-ratio):4.914693
Number of Samples:2 / 3
Experimental DFPM + ABA (Col-0)
Col-0 seedlings were grown for 12 days on plates, then transferred into a solution containing 30µM DFPM (5-(3,4-Dichlorophenyl)Furan-2-yl]-Piperidin-1-ylMethanethione) for 30min. 10µM ABA (abscisic acid) was then added to the solution and incubation was continued for 5.5h prior harvesting the whole seedlings. DFPM is a randomly synthesized compound that inhibits expresion of ABA-induced genes, e.g. RAB18. DFPM also inhibits some ABA-mediated physiological responses, e.g. ABA-induced stomatal closure.
Control solvent treated seedling samples (Col-0)
Seedling samples Col-0 grown for 12 days on plates, then transferred into a solution containing DMSO (1:5000 v/v) for 6h.

ABA study 12 (Col-0) / solvent treated seedling samples (Col-0)

Relative Expression (log2-ratio):4.840543
Number of Samples:3 / 3
Experimental ABA study 12 (Col-0)
Seedling samples Col-0 grown for 12 days on plates, then transferred into a solution containing 10µM ABA (abscisic acid) for 6h.
Control solvent treated seedling samples (Col-0)
Seedling samples Col-0 grown for 12 days on plates, then transferred into a solution containing DMSO (1:5000 v/v) for 6h.

ABA study 6 (Col-0) / untreated plant samples (Col-0)

Relative Expression (log2-ratio):3.9613428
Number of Samples:3 / 3
Experimental ABA study 6 (Col-0)
Plant samples of Col-0 grown for 2 weeks on MS agar medium with 3% sucrose (16h light / 8h dark cycles, 22°C) and then treated with 100μM ABA (abscisic acid) for 4h.
Control untreated plant samples (Col-0)
Plant samples of Col-0 grown for 2 weeks on MS agar medium with 3% sucrose (16h light / 8h dark cycles, 22°C).

germination (48h) / stratification (48h)

Relative Expression (log2-ratio):3.7422085
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control stratification (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, then sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), and stratified at 4°C in darkness for 48h.

ABA study 6 (srk2cf) / untreated plant samples (srk2cf)

Relative Expression (log2-ratio):3.6748266
Number of Samples:3 / 3
Experimental ABA study 6 (srk2cf)
Plant samples of srk2cf double mutant grown for 2 weeks on MS agar medium with 3% sucrose (16h light / 8h dark cycles, 22°C) and then treated with 100μM ABA (abscisic acid) for 4h.
Control untreated plant samples (srk2cf)
Plant samples of srk2cf double mutant grown for 2 weeks on MS agar medium with 3% sucrose (16h light / 8h dark cycles, 22°C).

ABA study 11 (3h) / solvent treated cell samples (3h)

Relative Expression (log2-ratio):3.3951654
Number of Samples:2 / 2
Experimental ABA study 11 (3h)
Cell samples of T87 cell suspension that was grown in modified JPL medium (Axelos et al., 1992, Plant Physiol Biochem. 30: 123-128) under 16h light / 8h dark cycles, 24°C, on a rotary shaker at 100rpm with subculturing every 7 days and then, at the exponential phase of growth, exposed to 25μM ABA (abscisic acid) for 3h.
Control solvent treated cell samples (3h)
Cell samples of T87 cell suspension that was grown in modified JPL medium (Axelos et al., 1992, Plant Physiol Biochem. 30: 123-128) under 16h light / 8h dark cycles, 24°C, on a rotary shaker at 100rpm with subculturing every 7 days and then, at the exponential phase of growth, exposed to 0.6% ethanol for 3h.

germination (48h) / seed desiccation

Relative Expression (log2-ratio):3.3779545
Number of Samples:3 / 3
Experimental germination (48h)
Col-0 seeds were harvested, allowed to dessicate for 15 days in darkness, sterilized, plated on Murashige and Skoog medium (containing 3% sucrose), stratified at 4°C in darkness for 48h, and then transferred to continuous light (100µmol photons m-2 s-1) at 22°C for 48h.
Control seed desiccation
Col-0 seeds were harvested and allowed to dessicate for 15 days in darkness.

ABA (3h) / mock treated seedlings (3h)

Relative Expression (log2-ratio):3.3312674
Number of Samples:2 / 2
Experimental ABA (3h)
Col-0 seedlings, treated with abscisic acid; 10uM ABA for 3h.
Control mock treated seedlings (3h)
Col-0 seedling samples, mock treated for 3h.