TOP TEN perturbations for Q9M7Q2 (Arabidopsis thaliana)

Organism: Arabidopsis thaliana
Gene: Q9M7Q2
Selected probe(set): 258026_at
Platform: Affymetrix Arabidopsis ATH1 Genome Array

Expression of Q9M7Q2 (258026_at) across 3243 perturbations tested by GENEVESTIGATOR:

drought study 17 (5d) / untreated Col-0 flower bud samples (5d)

Relative Expression (log2-ratio):2.59066
Number of Samples:2 / 2
Experimental drought study 17 (5d)
Closed flower bud samples of Col-0 plants grown without water limitation (90% soil moisture, i.e. 90g H2O per 100g dry soil mixture in a pot without drainage holes) for 24 days (beginning of bolting, the main stem approx.1cm high), then subjected to drought stress by withholding water for 3 days (at the end of this period soil moisture was approx. 35%) and maintaining 35% soil moisture for 2 more days (by adding the needed amount of water). In order to accelerate the soil water evaporation, relative air humidity in the chamber was maintained at 30% during the first 3 days of the drought-treatment. Other plant growth conditions: 1 plant per pot; soil mixture was Metro-Mix 360 soil (Sun Gro Horticulture Canada) and Turface profile greens grade (Profile Products) at 3 to 2 ratio (v:v); Conviron growth chamber, 16h light (300μmol photons m-2 s-1) / 8h dark, 22°C, 60% relative air humidity (except during the first 3 days of the drought-treatment).
Control untreated Col-0 flower bud samples (5d)
Closed flower bud samples of Col-0 plants grown without water limitation (90% soil moisture, i.e. 90g H2O per 100g dry soil mixture in a pot without drainage holes) for 29 days (flowering). Other plant growth conditions: 1 plant per pot; soil mixture was Metro-Mix 360 soil (Sun Gro Horticulture Canada) and Turface profile greens grade (Profile Products) at 3 to 2 ratio (v:v); Conviron growth chamber, 16h light (300μmol photons m-2 s-1) / 8h dark, 22°C, 60% relative air humidity.

bzr1-1Dxbri1-116 / bri1-116

Relative Expression (log2-ratio):2.5585947
Number of Samples:3 / 3
Experimental bzr1-1Dxbri1-116
bzr1-1Dbri1-116 double mutant in Col-0 background. bzr1-1D is a dominant mutation which stabilizes the BZR1 protein. The de-etiolated phenotype of the null bri1-116 mutant grown in the dark is completely suppressed by the bzr1-1D mutation.
Control bri1-116
bri1-116 null mutant in Col-0 background. bri1-116 is a severe BR-perception mutant.

MeJa study 5 (penta) / untreated leaf disc samples (penta)

Relative Expression (log2-ratio):-2.2784367
Number of Samples:3 / 3
Experimental MeJa study 5 (penta)
Leaf disc samples were collected from five weeks old penta mutant plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions, sprayed with 10µM Methyl Jasmonate solution, covered with plastic bags and kept for one hour.
Control untreated leaf disc samples (penta)
Leaf disc samples of five weeks old penta mutant plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions.

bzr1-1Dxbri1-116 / Col-0

Relative Expression (log2-ratio):2.2574692
Number of Samples:3 / 3
Experimental bzr1-1Dxbri1-116
bzr1-1Dbri1-116 double mutant in Col-0 background. bzr1-1D is a dominant mutation which stabilizes the BZR1 protein. The de-etiolated phenotype of the null bri1-116 mutant grown in the dark is completely suppressed by the bzr1-1D mutation.
Control Col-0
Arabidopsis accession Columbia-0. Origin: Poland; latitude of origin: 52°73'N, longitude of origin: 15°15'E. Col-0 is resistant to the isolate AG8 and susceptible to the isolate AG2-1 of soil-borne necrotrophic fungus Rhizoctonia solani. Freezing tolerance of non-acclimated Col-0 leaves: LT50 (temperature of 50% electrolyte leakage) = −5.34°C; freezing tolerance of Col-0 leaves after acclimation at 4°C under 16h photoperiod (90µmol photons m-2 s-1) for 14 days: LT50 = −9.68°C. Acclimated Col-0 leaves accumulate approx. 20µmol g FW-1 glucose, 10µmol g FW-1 fructose, 20µmol g FW-1 sucrose, 4µmol g FW-1 raffinose, 5.5µmol g FW-1 proline (Zuther et al., 2012, Plant Cell Environ. 35: 1860-1878). Col-0 is comparatively tolerant to ozone: ozone treated (400nl L-1 O3 for 6h) Col-0 shows less cell death than ozone treated Te-0 accession (Xu et al., 2015, Plant Cell Environ. 38: 1418-1433). Col-0 is susceptible to bacterial pathogen Pseudomonas syringae pv tomato (Pst) strain DC3000. As Col-0 carries the resistance gene RPS4 (At5g45250), it is resistant to Pst DC3000 expressing the bacterial effector avrRps4 (Howard et al., 2013, PLoS One 8: e74183). When 4-day-old Col-0 seedlings are exposed to mild drought stress (soil water content (SWC) of 1.2g H2O g-1 dry soil) for 6 days, area of their 3rd rosette leaf is reduced by approx. 30% compared to that of control Col-0 seedlings grown for 4+6 days at SWC of 2.2g H2O g-1 dry soil. When 4-day-old Col-0 seedlings are exposed to mild drought stress for 18 days (SWC is kept at 1.2g H2O g-1 dry soil for the first 6 days, then slowly decreases and is kept at 0.7g H2O g-1 dry soil till the end of the treatment), area of their 3rd rosette leaf is reduced by approx. 59% compared to that of control Col-0 plants grown for 4+18 days at SWC of 2.2g H2O g-1 dry soil (Clauw et al., 2015, Plant Physiol. 167: 800-816).

circadian clock study 9 (18h dark) / circadian clock study 9 (6h dark)

Relative Expression (log2-ratio):2.1023073
Number of Samples:3 / 3
Experimental circadian clock study 9 (18h dark)
Shoot samples of Col plants grown for 13.5 days on soil under 12h light / 12h dark cycles at 22°C, then, starting at the beginning of the dark period on day 14, kept for 18h in darkness (i.e. samples were harvested 6h after the beginning of the subjective light period on day 15).
Control circadian clock study 9 (6h dark)
Shoot samples of Col plants grown for 13.5 days on soil under 12h light / 12h dark cycles at 22°C, then, starting at the beginning of the dark period on day 14, kept for 6h in darkness.

drought study 17 (10d) / untreated Col-0 flower bud samples (10d)

Relative Expression (log2-ratio):2.0832996
Number of Samples:2 / 2
Experimental drought study 17 (10d)
Closed flower bud samples of Col-0 plants grown without water limitation (90% soil moisture, i.e. 90g H2O per 100g dry soil mixture in a pot without drainage holes) for 24 days (beginning of bolting, the main stem approx.1cm high), then subjected to drought stress by withholding water for 3 days (at the end of this period soil moisture was approx. 35%) and maintaining 35% soil moisture for 7 more days (by adding the needed amount of water). In order to accelerate the soil water evaporation, relative air humidity in the chamber was maintained at 30% during the first 3 days of the drought-treatment. Other plant growth conditions: 1 plant per pot; soil mixture was Metro-Mix 360 soil (Sun Gro Horticulture Canada) and Turface profile greens grade (Profile Products) at 3 to 2 ratio (v:v); Conviron growth chamber, 16h light (300μmol photons m-2 s-1) / 8h dark, 22°C, 60% relative air humidity (except during the first 3 days of the drought-treatment).
Control untreated Col-0 flower bud samples (10d)
Closed flower bud samples of Col-0 plants grown without water limitation (90% soil moisture, i.e. 90g H2O per 100g dry soil mixture in a pot without drainage holes) for 34 days (flowering). Other plant growth conditions: 1 plant per pot; soil mixture was Metro-Mix 360 soil (Sun Gro Horticulture Canada) and Turface profile greens grade (Profile Products) at 3 to 2 ratio (v:v); Conviron growth chamber, 16h light (300μmol photons m-2 s-1) / 8h dark, 22°C, 60% relative air humidity.

drought (wt) / untreated leaf samples (Col)

Relative Expression (log2-ratio):2.0402126
Number of Samples:2 / 2
Experimental drought (wt)
Rosette leaf samples of wild type (Columbia) plants, grown under normal watering conditions for 24 days and then stressed by completely depriving of irrigation for 10 days.
Control untreated leaf samples (Col)
Rosette leaf samples of wild type (Columbia) plants, grown under normal watering conditions for 34 days.

hypoxia study 17 (pCO2:HF-RPL18) / mock treated pCO2:HF-RPL18 root samples

Relative Expression (log2-ratio):1.9675198
Number of Samples:2 / 2
Experimental hypoxia study 17 (pCO2:HF-RPL18)
Ribosome-bound mRNA from root cortex of pCO2:HF-RPL18 seedlings that were grown for 7 days on vertically oriented plates with solid medium (0.43% (w/v) Murashige and Skoog salts, 1% (w/v) sucrose, 0.4% (w/v) phytagel; pH 5.7) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 23°C, then subjected to hypoxia stress by gassing the plates with 99.998% (v/v) argon for 2h in Lucite chambers at 23-25°C under dim light (5-7µmol photons m-2 s-1). Roots were harvested and ribosome-bound mRNA was isolated via immunopurification of ribosomes carrying FLAG-tagged RPL18 with the anti-FLAG antibody.
Control mock treated pCO2:HF-RPL18 root samples
Ribosome-bound mRNA from root cortex of pCO2:HF-RPL18 seedlings that were grown for 7 days on vertically oriented plates with solid medium (0.43% (w/v) Murashige and Skoog salts, 1% (w/v) sucrose, 0.4% (w/v) phytagel; pH 5.7) under 16h light (80µmol photons m-2 s-1) / 8h dark cycles at 23°C, then placed for 2h under dim light (5-7µmol photons m-2 s-1) at 23-25°C. Roots were harvested and ribosome-bound mRNA was isolated via immunopurification of ribosomes carrying FLAG-tagged RPL18 with the anti-FLAG antibody.

MeJa study 5 (Ler) / untreated leaf disc samples (Ler)

Relative Expression (log2-ratio):-1.7938185
Number of Samples:3 / 3
Experimental MeJa study 5 (Ler)
Leaf disc samples were collected from five weeks old Ler plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions, sprayed with 10µM Methyl Jasmonate solution, covered with plastic bags and kept for one hour.
Control untreated leaf disc samples (Ler)
Leaf disk samples of five weeks old Ler plants grown under short day (8h light / 16h dark, 120μmols-1m-2) conditions.

drought study 14 (Col-0) / untreated Col-0 rosette leaf samples

Relative Expression (log2-ratio):1.7664461
Number of Samples:3 / 3
Experimental drought study 14 (Col-0)
Rosette leaf samples of Col-0 grown for 21 day on solerite soil without water limitation, then subjected to drought stress by withholding water for 9 days (soil moisture dropped to 30%). Other growth conditions: 16h light (120µmol photons m-2 s-1) / 8h dark cycles; 22°C.
Control untreated Col-0 rosette leaf samples
Rosette leaf samples of Col-0 grown for 30 days on solerite soil without water limitation under 16h light (120µmol photons m-2 s-1) / 8h dark cycles at 22°C.