TOP TEN perturbations for 1005_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1005_at
Selected probe(set): 201041_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1005_at (201041_s_at) across 6537 perturbations tested by GENEVESTIGATOR:

actinomycin D study 1 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):-6.843176
Number of Samples:3 / 3
Experimental actinomycin D study 1
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. ATC code:
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture.

LPS study 4 / mock treated MONO-MAC-6 cell sample

Relative Expression (log2-ratio):6.1191797
Number of Samples:2 / 2
Experimental LPS study 4
MONO-MAC-6 (MM6) cells were treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells mock treated.

LPS study 4 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample

Relative Expression (log2-ratio):5.950343
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA contr.)
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated / transduced MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.

LPS study 4 (shRNA cycT1) / cycT1 depletion study 2 (shRNA)

Relative Expression (log2-ratio):5.3060646
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA cycT1)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control cycT1 depletion study 2 (shRNA)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then mock treated.

heat shock study 3 / untreated THP-1 cell sample

Relative Expression (log2-ratio):5.2578707
Number of Samples:3 / 3
Experimental heat shock study 3
Cultured THP-1 mononuclear cells were treated with heat shock (43°C) for 1 hour followed by a 4 hour recovery period at 37°C.
Control untreated THP-1 cell sample
Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C.

bladder cancer study 1 / normal bladder tissue

Relative Expression (log2-ratio):-4.7898417
Number of Samples:3 / 3
Experimental bladder cancer study 1
Low grade superficial tumor samples.
Control normal bladder tissue
Normal bladder tissue.

echinomycin study 1 / deferoxamine study 5

Relative Expression (log2-ratio):-4.744938
Number of Samples:3 / 3
Experimental echinomycin study 1
Echinomycin (100nM; 2h) treated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:---
Control deferoxamine study 5
Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:

Wnt3a study 1 / normal B-cell sample (CD19+)

Relative Expression (log2-ratio):-4.733987
Number of Samples:4 / 20
Experimental Wnt3a study 1
CD19+ B-cell samples from normal control subjects and treated with Wnt3a for 48 hours. It is not clear whether the concentration of Wnt3a was 5ng/ml or 50ng/ml. B-cells were isolated from peripheral blood mononuclear cells and cultured in B-cell media.
Control normal B-cell sample (CD19+)
CD19+ B-cell samples from normal control subjects. B-cells were isolated from peripheral blood mononuclear cells.

T-cell activation study 3 / resting CD4 T-lymphocyte (crude fraction) sample

Relative Expression (log2-ratio):-4.671398
Number of Samples:2 / 2
Experimental T-cell activation study 3
CD4+ T-cell samples prepared from crude lymphocyte fraction. Cells were activated with anti-CD3/28 beads for 18hrs.
Control resting CD4 T-lymphocyte (crude fraction) sample
Resting CD4 T-lymphocytes prepared from crude lymphocyte fraction.

brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample

Relative Expression (log2-ratio):4.359271
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116)
Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 cell sample
Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.