TOP TEN perturbations for 1025_g_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1025_g_at
Selected probe(set): 205749_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1025_g_at (205749_at) across 6672 perturbations tested by GENEVESTIGATOR:

smoking study 55 (24 hrs) / normal sham-exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):8.004061
Number of Samples:3 / 3
Experimental smoking study 55 (24 hrs)
Bronchial epithelium organotypic tissue culture harvested 24 hours (recovery phase) after 4 repetitive exposures to 16% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.
Control normal sham-exposed bronchial epithelial cell sample
Bronchial epithelium organotypic tissue culture harvested 24 hours (recovery phase) after 4 single exposures to 100% humidified air (with 60% humidity), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts. The organotypic tissue culture model was maintained for 14 days at 37°C and culture medium replaced every 2 days.

smoking study 53 (28 min) / normal sham-exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):7.9933434
Number of Samples:3 / 3
Experimental smoking study 53 (28 min)
Normal human bronchial/tracheal epithelial (NHBE) cells were treated for 28 minutes with diluted cigarette smoke (15% v/v) followed by 24 hours recovery phase with fresh culture media. NHBE cells were cultured as organotypic cultures allowing an air-liquid interface. Seven puffs per cigarette (3R4F reference Kentucky cigarettes) and one puff per minute of exposure were used, resulting in 7.74 µg of Total Particulate Matter and 8.3 µg of CO / well /minute inside the exposure chamber. Cigarette smoke was generated on the VC 10 smoking robot in compliance with the International Organization for Standardization smoking regimen. Number of cigarettes varied to adjust to the exposure times.
Control normal sham-exposed bronchial epithelial cell sample
Normal human bronchial/tracheal epithelial (NHBE) cells were sham-exposed for 28 minutes to 100 % synthetic air (v/v; 15% oxygen and 85% nitrogen) followed by 24 hours recovery phase with fresh culture media. NHBE cells were cultured as organotypic cultures allowing an air-liquid interface.

smoking study 47 (21 min) / normal sham-exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):7.8683786
Number of Samples:3 / 3
Experimental smoking study 47 (21 min)
Normal human bronchial/tracheal epithelial (NHBE) cells were treated for 21 minutes with diluted cigarette smoke (15% v/v) followed by 4 hours recovery phase with fresh culture media. NHBE cells were cultured as organotypic cultures allowing an air-liquid interface. Seven puffs per cigarette (3R4F reference Kentucky cigarettes) and one puff per minute of exposure were used, resulting in 7.74 µg of Total Particulate Matter and 8.3 µg of CO / well /minute inside the exposure chamber. Cigarette smoke was generated on the VC 10 smoking robot in compliance with the International Organization for Standardization smoking regimen. Number of cigarettes varied to adjust to the exposure times.
Control normal sham-exposed bronchial epithelial cell sample
Normal human bronchial/tracheal epithelial (NHBE) cells were sham-exposed for 21 minutes to 100 % synthetic air (v/v; 15% oxygen and 85% nitrogen) followed by 4 hours recovery phase with fresh culture media. NHBE cells were cultured as organotypic cultures allowing an air-liquid interface.

smoking study 83 (non-smoker) / untreated lung epithelial culture sample (non-smoker)

Relative Expression (log2-ratio):7.7922945
Number of Samples:2 / 2
Experimental smoking study 83 (non-smoker)
Air-liquid interface lung epithelial culture exposed to cigarette smoke (30 mins exposure on four separate days). Lung epithelial cells were isolated from non-smokers and cultures were placed in smoking chambers with basal surface in contact with media. They were continually exposed to smoke (from filtered Kentucky reference (3R4F) cigarettes) for 30mins on days 1, 2, 5 and 6 (smoke/air dilution of 1:10 or 1:20).
Control untreated lung epithelial culture sample (non-smoker)
Untreated air-liquid interface lung epithelial culture. Lung epithelial cells were isolated from non-smokers and cultures were maintained in proprietary media.

TCDD study 1 (24h) / vehicle (DMSO) treated HepG2 cell sample

Relative Expression (log2-ratio):7.7111797
Number of Samples:3 / 7
Experimental TCDD study 1 (24h)
HepG2 cells exposed to 10nM 2,3,7,8-tetrachloro dibenzo-p-dioxin (TCDD) in DMSO solvent for 24 hours. ATC code:---
Control vehicle (DMSO) treated HepG2 cell sample
HepG2 cells exposed to DMSO solvent for 24 hours.

smoking study 65 (24h) / normal sham-exposed bronchial organotypic tissue

Relative Expression (log2-ratio):7.710574
Number of Samples:3 / 3
Experimental smoking study 65 (24h)
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 24 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed bronchial organotypic tissue
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 24 hours. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

smoking study 80 (small airway epith; CHTP1.2; 24h; 0.15mg/l) / smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)

Relative Expression (log2-ratio):-7.6403017
Number of Samples:6 / 5
Experimental smoking study 80 (small airway epith; CHTP1.2; 24h; 0.15mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to 0.15 mg nicotine/l aerosol from a potential modified-risk tobacco product, carbon-heated tobacco product 1.2 (CHTP1.2). Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The aerosol was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each CHTP1.2 stick was used for a total of 12 puffs per stick. The cultures were exposed to aerosol continuously for 28 minutes in smoking machine and 24 hours after aerosol exposure the cells were collected for RNA extraction.
Control smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 24 hours after smoke exposure the cells were collected for RNA extraction.

smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l) / air exposed organotypic small airway epithelium culture sample (24h; 3R4F)

Relative Expression (log2-ratio):7.606591
Number of Samples:5 / 5
Experimental smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 24 hours after smoke exposure the cells were collected for RNA extraction.
Control air exposed organotypic small airway epithelium culture sample (24h; 3R4F)
Organotypic human small airway epithelium culture 24 hours after exposure to conditioned fresh air. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for 3R4F smoke exposure at timepoint 24 hours after exposure.

smoking study 66 (24h) / normal sham-exposed nasal organotypic tissue

Relative Expression (log2-ratio):7.573682
Number of Samples:3 / 3
Experimental smoking study 66 (24h)
Human nasal organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 24 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed nasal organotypic tissue
Human nasal organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 24 hours. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

TCDD study 1 (48h) / vehicle (DMSO) treated HepG2 cell sample

Relative Expression (log2-ratio):7.505664
Number of Samples:3 / 19
Experimental TCDD study 1 (48h)
HepG2 cells exposed to 10nM 2,3,7,8-tetrachloro dibenzo-p-dioxin (TCDD) in DMSO solvent for 48 hours. ATC code:---
Control vehicle (DMSO) treated HepG2 cell sample
HepG2 cells exposed to DMSO solvent for 48 hours.