TOP TEN perturbations for 1045_s_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1045_s_at
Selected probe(set): 201783_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1045_s_at (201783_s_at) across 6593 perturbations tested by GENEVESTIGATOR:

echinomycin study 1 / deferoxamine study 5

Relative Expression (log2-ratio):-2.3659153
Number of Samples:3 / 3
Experimental echinomycin study 1
Echinomycin (100nM; 2h) treated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:---
Control deferoxamine study 5
Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:

TNF-ɑ study 20 / normal monocyte sample

Relative Expression (log2-ratio):2.1298742
Number of Samples:3 / 7
Experimental TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

TNF-ɑ study 20 / unstimulated monocyte sample

Relative Expression (log2-ratio):1.8907051
Number of Samples:3 / 11
Experimental TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.
Control unstimulated monocyte sample
Monocyte samples isolated from healthy donors and incubated in whole blood for 1.5 hour without any stimulation. Following incubation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

rheumatoid arthritis study 31 / TNF-ɑ study 20

Relative Expression (log2-ratio):-1.8897085
Number of Samples:4 / 3
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocyte from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocyte were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

prostate cancer study 8 (ptasc) / prostate cancer study 8 (psfmc)

Relative Expression (log2-ratio):1.8696527
Number of Samples:2 / 5
Experimental prostate cancer study 8 (ptasc)
CD90+ FACS sorted prostate tumor-associated stromal cell (ptasc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (psfmc)
CD49a+ FACS sorted prostate stromal fibromuscular cell (psfmc) samples from patients with primary prostate cancer collected after radical prostatectomy.

E. coli study 1 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):1.7518854
Number of Samples:12 / 16
Experimental E. coli study 1
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 × 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.

male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):1.6466236
Number of Samples:2 / 8
Experimental male infertility study 1 (juvenile; Ad-)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

chronic obstructive pulmonary disease study 21 (smoker; stim. blood culture) / chronic obstructive pulmonary disease study 21 (smoker; unstim. blood culture)

Relative Expression (log2-ratio):1.6453772
Number of Samples:42 / 46
Experimental chronic obstructive pulmonary disease study 21 (smoker; stim. blood culture)
Stimulated peripheral blood cultures from patients with COPD. Persons included in the study were former or current smokers with smoking status > 10 pack-yr and with FEV1/FVC < 70 post bronchodilator. One milliliter of peripheral blood was collected into anti-CD3 and anti-CD28 TruCulture tubes and incubated for 24 hours at 37ºC. The TruCulture is an ex-vivo culture system which should preserve physiological cellular responses of immune cells to stress or stimulation at the time and place of sample collection and minimize the bias and variability caused by sample manipulation. Available with/without specific stimulant.
Control chronic obstructive pulmonary disease study 21 (smoker; unstim. blood culture)
Unstimulated peripheral blood cultures from patients with COPD. Persons included in the study were former or current smokers with smoking status > 10 pack-yr and with FEV1/FVC < 70 post bronchodilator. One milliliter of peripheral blood was collected into null TruCulture tubes and incubated for 24 hours at 37ºC. The TruCulture is an ex-vivo culture system which should preserve physiological cellular responses of immune cells to stress or stimulation at the time and place of sample collection and minimize the bias and variability caused by sample manipulation. Available with/without specific stimulant.

E. coli study 2 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):1.6106348
Number of Samples:5 / 7
Experimental E. coli study 2
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 × 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.

male infertility study 1 (juvenile; Ad+) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):1.5724869
Number of Samples:5 / 8
Experimental male infertility study 1 (juvenile; Ad+)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained typical level of A-dark (Ad+) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).