TOP TEN perturbations for 1092_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1092_at
Selected probe(set): 206758_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1092_at (206758_at) across 6672 perturbations tested by GENEVESTIGATOR:

hypoxia study 5 / untreated pancreas carcinoma cell line (FG) sample

Relative Expression (log2-ratio):3.8294115
Number of Samples:3 / 3
Experimental hypoxia study 5
Human metastatic pancreas carcinoma cell line FG with low metastatic potential in xenografts grown in culture under reduced oxygen.
Control untreated pancreas carcinoma cell line (FG) sample
Human metastatic pancreas carcinoma cell line FG with low metastatic potential in xenografts grown in culture under normoxia.

oxLDL study 4 / untreated retina pigment epithelial cell (APRE-19) sample

Relative Expression (log2-ratio):-3.2966385
Number of Samples:3 / 3
Experimental oxLDL study 4
Retina pigment epithelial cells (APRE-19) cells were exposed to oxidatively modified low density lipoproteins (ox-LDL) for 48 hours.
Control untreated retina pigment epithelial cell (APRE-19) sample
Retina pigment epithelial cells (APRE-19) cells without any treatment.

HIF-2a depletion study 1 (hypoxia; HK2) / hypoxia study 11 (HK2)

Relative Expression (log2-ratio):-2.6871338
Number of Samples:3 / 3
Experimental HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-2a (Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control hypoxia study 11 (HK2)
Proximal tubule epithelial cell line HK-2 treated with hypoxia (1% O2) for 24 hours. HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

HIF-1a depletion study 2 (hypoxia; HK2) / HIF-1a depletion study 2 (normoxia; HK2)

Relative Expression (log2-ratio):2.4338722
Number of Samples:3 / 3
Experimental HIF-1a depletion study 2 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control HIF-1a depletion study 2 (normoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with normoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

HIF-2a depletion study 1 (hypoxia; HK2) / HIF-1a depletion study 2 (hypoxia; HK2)

Relative Expression (log2-ratio):-2.3817034
Number of Samples:3 / 3
Experimental HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-2a (Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control HIF-1a depletion study 2 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) / hypoxia study 11 (HK2)

Relative Expression (log2-ratio):-2.2926178
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control hypoxia study 11 (HK2)
Proximal tubule epithelial cell line HK-2 treated with hypoxia (1% O2) for 24 hours. HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

keratinocyte differentiation study 2 (KLF4 siRNA) / keratinocyte differentiation study 2

Relative Expression (log2-ratio):2.0467215
Number of Samples:2 / 2
Experimental keratinocyte differentiation study 2 (KLF4 siRNA)
KLF4 (Kruppel-like factor 4) depleted primary neonatal keratinocytes differentiated with 1.2 mM calcium for 3 days. Keratinocytes obtained from freshly isolated foreskin were grown in keratinocyte-SFM medium supplemented with epidermal growth factor and bovine pituitary extract. KLF4 depletion was done using siRNAs: KLF4i(A): CCGAGGAGTTCAACGATCT; KLF4i(B): TGACCAGGCACTACCGTAA. Differentiation was induced at 100% confluency.
Control keratinocyte differentiation study 2
Primary neonatal keratinocytes differentiated with 1.2 mM calcium for 3 days. Keratinocytes obtained from freshly isolated foreskin were grown in keratinocyte-SFM medium supplemented with epidermal growth factor and bovine pituitary extract. Differentiation was induced at 100% confluency.

CREB1 depletion study 2 (NCI-H3118) / control transduced NCI-H3118 cell sample

Relative Expression (log2-ratio):2.0130901
Number of Samples:3 / 3
Experimental CREB1 depletion study 2 (NCI-H3118)
NCI-H3118 cells with shRNA mediated knock down of CREB1. Cells were infected on two consecutive dates with the lentiviruses in fresh complete medium (DMEM, 10% FBS) plus 8ug/ml polybrene for 6 to 8 hours and replaced with fresh medium. Cells were harvested at 72 hours after the 1st infection.
Control control transduced NCI-H3118 cell sample
H3118 cells expressing a control shRNA targeting luciferase gene (shLuc). Cells were infected on two consecutive dates with the lentiviruses in fresh complete medium (DMEM, 10% FBS) plus 8ug/ml polybrene for 6 to 8 hours and replaced with fresh medium. Cells were harvested at 72 hours after the 1st infection.

basal cell carcinoma study 2 / normal skin tissue

Relative Expression (log2-ratio):2.0060139
Number of Samples:2 / 64
Experimental basal cell carcinoma study 2
Primary tumor tissue from the skin of patients with basal cell carcinoma (BCC).
Control normal skin tissue
Normal skin samples from healthy donors.

HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) / HIF-1a depletion study 2 (hypoxia; HK2)

Relative Expression (log2-ratio):-1.9871874
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control HIF-1a depletion study 2 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.