TOP TEN perturbations for 1173_g_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1173_g_at
Selected probe(set): 210592_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1173_g_at (210592_s_at) across 6537 perturbations tested by GENEVESTIGATOR:

doxorubicin study 3 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):4.4857254
Number of Samples:2 / 2
Experimental doxorubicin study 3
MCF7 cells treated with 2uM doxorubicin for 24 hours. ATC code:
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

heat shock study 1 (LPS) / untreated THP-1 cell sample

Relative Expression (log2-ratio):4.068989
Number of Samples:3 / 3
Experimental heat shock study 1 (LPS)
Cultured THP-1 mononuclear cells were treated with heat shock (43°C; 1h) and lipopolysaccharide (1ug/ml; 4h).
Control untreated THP-1 cell sample
Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C.

LPS study 4 / mock treated MONO-MAC-6 cell sample

Relative Expression (log2-ratio):3.59453
Number of Samples:2 / 2
Experimental LPS study 4
MONO-MAC-6 (MM6) cells were treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells mock treated.

LPS study 4 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample

Relative Expression (log2-ratio):3.4864492
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA contr.)
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated / transduced MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.

BPDE study 3 (0.5uM) / vehicle (DMSO) treated WI-38 fibroblast sample

Relative Expression (log2-ratio):3.318656
Number of Samples:3 / 3
Experimental BPDE study 3 (0.5uM)
WI-38 normal fibroblast cells exposed to 0.5uM racemic anti-BPDE [(±)-7r, 8t-dihydroxy-9t, 10t-epoxy-7, 8, 9, 10-tetrahydro benzo[a]pyrene] (BPDE) for 24 hours. Cells were incubated with BPDE (added in 10 ul DMSO) for 20 min, the exposure medium was then replaced with fresh medium, and after 24 hours cells were washed with phosphate-buffered saline and harvested. ATC code:---
Control vehicle (DMSO) treated WI-38 fibroblast sample
WI-38 normal fibroblast cells exposed to 0.1% DMSO vehicle control for 24 hours.

LPS study 2 / untreated THP-1 cell sample

Relative Expression (log2-ratio):3.3032684
Number of Samples:3 / 3
Experimental LPS study 2
Cultured THP-1 mononuclear cells were treated with lipopolysaccharide (1 microgram/ml) for 4 hours at 37°C. ATC code:---
Control untreated THP-1 cell sample
Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C.

LPS study 4 (shRNA cycT1) / cycT1 depletion study 2 (shRNA)

Relative Expression (log2-ratio):3.2584372
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA cycT1)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control cycT1 depletion study 2 (shRNA)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then mock treated.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):-3.2226715
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

retina pigment epithelium study 1 (adult) / retina pigment epithelium study 1 (fetal)

Relative Expression (log2-ratio):3.1826935
Number of Samples:2 / 6
Experimental retina pigment epithelium study 1 (adult)
Postmortem human native retina pigment epithelium samples from adult individuals.
Control retina pigment epithelium study 1 (fetal)
Human native fetal retina pigment epithelium samples obtained at gestation age between week 16-18.

HCC study 20 (CDX; Hep-G2; ectopic) / HCC study 20 (PDX; ectopic)

Relative Expression (log2-ratio):3.1612291
Number of Samples:3 / 11
Experimental HCC study 20 (CDX; Hep-G2; ectopic)
Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks.
Control HCC study 20 (PDX; ectopic)
Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks.