TOP TEN perturbations for 11759665_a_at (Homo sapiens)

Organism: Homo sapiens
Gene: 11759665_a_at
Selected probe(set): 1554593_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 11759665_a_at (1554593_s_at) across 5880 perturbations tested by GENEVESTIGATOR:

atopic dermatitis study 21 (lesional; whole skin) / normal skin tissue

Relative Expression (log2-ratio):-4.781888
Number of Samples:5 / 6
Experimental atopic dermatitis study 21 (lesional; whole skin)
Lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %.
Control normal skin tissue
Full thickness skin samples isolated from healthy subjects by laser capture microdissection.

wound healing study 2 (ex vivo; DMSO) / normal skin tissue (ex vivo)

Relative Expression (log2-ratio):-4.16206
Number of Samples:3 / 3
Experimental wound healing study 2 (ex vivo; DMSO)
Ex vivo skin samples obtained from healthy donors following reduction surgery of abdomen, and incubated in culture medium containing DMSO for 4 days. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection. Skin was sliced into 1x10 mm slices and incubated in keratinocyte medium for four days with 1:1000 fold dilution of DMSO. The cultivation was performed in serum-free keratinocyte medium supplemented with transferrin, hEGF (0.15 ng/mL), 0.5 mg/mL hydrocortisone, gentamicin, amphotericin B, and epinephrine but without insulin.
Control normal skin tissue (ex vivo)
Normal skin samples obtained from healthy donors following reduction surgery of abdomen. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection.

atopic dermatitis study 21 (non-lesional; whole skin) / normal skin tissue

Relative Expression (log2-ratio):-4.0642996
Number of Samples:5 / 6
Experimental atopic dermatitis study 21 (non-lesional; whole skin)
Non-lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %.
Control normal skin tissue
Full thickness skin samples isolated from healthy subjects by laser capture microdissection.

brain tumor study 1 (ependymoma) / normal brain tissue

Relative Expression (log2-ratio):-3.9562154
Number of Samples:46 / 12
Experimental brain tumor study 1 (ependymoma)
Primary tumor tissue sample from the brain of patients with ependymoma.
Control normal brain tissue
Histologically normal and non-neoplastic tissue sample from different brain anatomical sites of patients with primary brain tumors.

RhoA overexpr. study 1 (G14V) / mammary epithelial cell sample

Relative Expression (log2-ratio):3.9111795
Number of Samples:3 / 3
Experimental RhoA overexpr. study 1 (G14V)
Human mammary epithelial cells (hMEC) immortalized by retroviral transfection with a constitutively active RhoA mutant (G14V). Cells were cultivated in DFCI-I medium with 100ug/ml of G418.
Control mammary epithelial cell sample
Human mammary epithelial cells (hMEC) obtained during reduction mammoplasty. Cultivated in DFCI-I medium.

skin transplantation study 1 (3d) / normal skin tissue (skin grafting)

Relative Expression (log2-ratio):-3.786828
Number of Samples:6 / 8
Experimental skin transplantation study 1 (3d)
Human skin punch biopsy from patients after skin grafting. Sample was taken at the site of re-epithelialization 3 days after surgery.
Control normal skin tissue (skin grafting)
Skin biopsy samples from healthy control subjects after breast or abdomen reduction surgery.

bladder cancer study 3 / normal bladder tissue

Relative Expression (log2-ratio):3.7577333
Number of Samples:3 / 3
Experimental bladder cancer study 3
High grade muscle invasive tumors.
Control normal bladder tissue
Normal bladder tissue.

medulloblastoma study 2 / non-tumor brain tissue

Relative Expression (log2-ratio):-3.620121
Number of Samples:4 / 9
Experimental medulloblastoma study 2
Primary tumor tissue sample from the infratentorial brain of pediatric patients with large-cell anaplastic medulloblastoma.
Control non-tumor brain tissue
Histologically normal brain tissue at rapid autopsy from patients who died from atypical teratoid/rhabdoid tumor.

brain tumor study 1 (pilocytic astrocytoma) / normal brain tissue

Relative Expression (log2-ratio):-3.3901143
Number of Samples:15 / 12
Experimental brain tumor study 1 (pilocytic astrocytoma)
Primary tumor tissue sample from the brain of patients with pilocytic astrocytoma.
Control normal brain tissue
Histologically normal and non-neoplastic tissue sample from different brain anatomical sites of patients with primary brain tumors.

bladder cancer study 3 / bladder cancer study 1

Relative Expression (log2-ratio):3.3282824
Number of Samples:3 / 3
Experimental bladder cancer study 3
High grade muscle invasive tumors.
Control bladder cancer study 1
Low grade superficial tumor samples.