TOP TEN perturbations for 117_at (Homo sapiens)

Organism: Homo sapiens
Gene: 117_at
Selected probe(set): 213418_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 117_at (213418_at) across 6673 perturbations tested by GENEVESTIGATOR:

epoxomicin study 1 (late) / untreated HEK293 cell sample

Relative Expression (log2-ratio):9.0942955
Number of Samples:4 / 4
Experimental epoxomicin study 1 (late)
Human embryonic kidney cells (HEK293) treated with 1 uM epoxomicin for 6 hours. ATC code:---
Control untreated HEK293 cell sample
Untreated human embryonic kidney cells (HEK293) at time point 0 hours.

heat shock study 3 / untreated THP-1 cell sample

Relative Expression (log2-ratio):8.84142
Number of Samples:3 / 3
Experimental heat shock study 3
Cultured THP-1 mononuclear cells were treated with heat shock (43°C) for 1 hour followed by a 4 hour recovery period at 37°C.
Control untreated THP-1 cell sample
Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C.

tumor supernatant activation study 3 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):8.768977
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

tumor supernatant activation study 3 / memory T-cell activation study 1

Relative Expression (log2-ratio):8.664005
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control memory T-cell activation study 1
Memory (CD45RA-) CD4+ T-cells isolated from peripheral blood of healthy female donors were activated with anti-CD3/CD28 beads for 24hrs.

elesclomol; paclitaxel study 1 / vehicle (DMSO) treated Hs 294T cell sample

Relative Expression (log2-ratio):8.581528
Number of Samples:2 / 2
Experimental elesclomol; paclitaxel study 1
Hs 294T cells (passage 6) treated with 100nM elesclomol and 250nM paclitaxel at timepoint 6 hours. ATC code:---
Control vehicle (DMSO) treated Hs 294T cell sample
Hs 294T cells (passage 6) treated with 0.01% DMSO at timepoint 6 hours.

elesclomol study 1 / vehicle (DMSO) treated Hs 294T cell sample

Relative Expression (log2-ratio):8.420677
Number of Samples:2 / 2
Experimental elesclomol study 1
Hs 294T cells (passage 6) treated with 100nM elesclomol at timepoint 6 hours. ATC code:---
Control vehicle (DMSO) treated Hs 294T cell sample
Hs 294T cells (passage 6) treated with 0.01% DMSO at timepoint 6 hours.

elesclomol study 2 / vehicle (DMSO) treated Hs 294T cell sample

Relative Expression (log2-ratio):8.404371
Number of Samples:2 / 2
Experimental elesclomol study 2
Hs 294T cells (passage 11) treated with 100nM elesclomol at timepoint 6 hours. ATC code:---
Control vehicle (DMSO) treated Hs 294T cell sample
Hs 294T cells (passage 11) treated with 0.01% DMSO at timepoint 6 hours.

smoking study 65 (4h) / normal sham-exposed bronchial organotypic tissue

Relative Expression (log2-ratio):8.087883
Number of Samples:3 / 3
Experimental smoking study 65 (4h)
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 4 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed bronchial organotypic tissue
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 4 hours. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

smoking study 11 (late) / filtered air treated bronchial epithelial cell sample (non-smoker)

Relative Expression (log2-ratio):7.7631073
Number of Samples:9 / 9
Experimental smoking study 11 (late)
Bronchial epithelial cells from non-smoker donors exposed to 1:50 dose (1.84ug/cm2) of diluted mainstream cigarette smoke for 1 hour following a 23 hours recovery period.
Control filtered air treated bronchial epithelial cell sample (non-smoker)
Bronchial epithelial cells from non-smoker donors exposed to filtered for 1 hour following a 23 hours recovery period.

allyl alcohol study 3 (70uM) / vehicle (medium) treated hepatocyte sample

Relative Expression (log2-ratio):7.638132
Number of Samples:2 / 2
Experimental allyl alcohol study 3 (70uM)
Hepatocytes treated with compound: allyl alcohol (70uM; CHEMBL234926) for 24 hours. ATC code:---
Control vehicle (medium) treated hepatocyte sample
Hepatocytes treated with vehicle (medium) for 24 hours.

Organism: Homo sapiens
Gene: 117_at
Selected probe(set): 213418_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 117_at (213418_at) across 6673 perturbations tested by GENEVESTIGATOR:

epoxomicin study 1 (late) / untreated HEK293 cell sample

Relative Expression (log2-ratio):9.0942955
Number of Samples:4 / 4
Experimental epoxomicin study 1 (late)
Human embryonic kidney cells (HEK293) treated with 1 uM epoxomicin for 6 hours. ATC code:---
Control untreated HEK293 cell sample
Untreated human embryonic kidney cells (HEK293) at time point 0 hours.

heat shock study 3 / untreated THP-1 cell sample

Relative Expression (log2-ratio):8.84142
Number of Samples:3 / 3
Experimental heat shock study 3
Cultured THP-1 mononuclear cells were treated with heat shock (43°C) for 1 hour followed by a 4 hour recovery period at 37°C.
Control untreated THP-1 cell sample
Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C.

tumor supernatant activation study 3 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):8.768977
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

tumor supernatant activation study 3 / memory T-cell activation study 1

Relative Expression (log2-ratio):8.664005
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control memory T-cell activation study 1
Memory (CD45RA-) CD4+ T-cells isolated from peripheral blood of healthy female donors were activated with anti-CD3/CD28 beads for 24hrs.

elesclomol; paclitaxel study 1 / vehicle (DMSO) treated Hs 294T cell sample

Relative Expression (log2-ratio):8.581528
Number of Samples:2 / 2
Experimental elesclomol; paclitaxel study 1
Hs 294T cells (passage 6) treated with 100nM elesclomol and 250nM paclitaxel at timepoint 6 hours. ATC code:---
Control vehicle (DMSO) treated Hs 294T cell sample
Hs 294T cells (passage 6) treated with 0.01% DMSO at timepoint 6 hours.

elesclomol study 1 / vehicle (DMSO) treated Hs 294T cell sample

Relative Expression (log2-ratio):8.420677
Number of Samples:2 / 2
Experimental elesclomol study 1
Hs 294T cells (passage 6) treated with 100nM elesclomol at timepoint 6 hours. ATC code:---
Control vehicle (DMSO) treated Hs 294T cell sample
Hs 294T cells (passage 6) treated with 0.01% DMSO at timepoint 6 hours.

elesclomol study 2 / vehicle (DMSO) treated Hs 294T cell sample

Relative Expression (log2-ratio):8.404371
Number of Samples:2 / 2
Experimental elesclomol study 2
Hs 294T cells (passage 11) treated with 100nM elesclomol at timepoint 6 hours. ATC code:---
Control vehicle (DMSO) treated Hs 294T cell sample
Hs 294T cells (passage 11) treated with 0.01% DMSO at timepoint 6 hours.

smoking study 65 (4h) / normal sham-exposed bronchial organotypic tissue

Relative Expression (log2-ratio):8.087883
Number of Samples:3 / 3
Experimental smoking study 65 (4h)
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 4 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed bronchial organotypic tissue
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 4 hours. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

smoking study 11 (late) / filtered air treated bronchial epithelial cell sample (non-smoker)

Relative Expression (log2-ratio):7.7631073
Number of Samples:9 / 9
Experimental smoking study 11 (late)
Bronchial epithelial cells from non-smoker donors exposed to 1:50 dose (1.84ug/cm2) of diluted mainstream cigarette smoke for 1 hour following a 23 hours recovery period.
Control filtered air treated bronchial epithelial cell sample (non-smoker)
Bronchial epithelial cells from non-smoker donors exposed to filtered for 1 hour following a 23 hours recovery period.

allyl alcohol study 3 (70uM) / vehicle (medium) treated hepatocyte sample

Relative Expression (log2-ratio):7.638132
Number of Samples:2 / 2
Experimental allyl alcohol study 3 (70uM)
Hepatocytes treated with compound: allyl alcohol (70uM; CHEMBL234926) for 24 hours. ATC code:---
Control vehicle (medium) treated hepatocyte sample
Hepatocytes treated with vehicle (medium) for 24 hours.