TOP TEN perturbations for 1211_s_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1211_s_at
Selected probe(set): 209833_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1211_s_at (209833_at) across 6540 perturbations tested by GENEVESTIGATOR:

CD44s overexpr. study 1 / normal HEK-293 cell sample

Relative Expression (log2-ratio):2.5601902
Number of Samples:3 / 3
Experimental CD44s overexpr. study 1
Human embryonic kidney cell line HEK-293 transfected with pcDNA3.1(-) vector containing codon-optimized human CD44 sequence for expression.
Control normal HEK-293 cell sample
Untransfected, native human embryonic kidney cell line HEK-293 cell samples.

PMA; ionomycine study 2 / unstimulated CD4 memory T-cell sample

Relative Expression (log2-ratio):2.4547234
Number of Samples:8 / 8
Experimental PMA; ionomycine study 2
CD4+ memory T cells derived from peripheral blood of healthy subjects were stimulated for 3 hours with phorbol 12-myristate 13-acetate (PMA, 10 ng/ml) and ionomycin (1 µg/ml). ATC code:---
Control unstimulated CD4 memory T-cell sample
Unstimulated CD4+ memory T cells derived from peripheral blood of healthy subjects.

monocyte co-culture study 1 (HS27a) / monocyte co-culture study 1 (HS5)

Relative Expression (log2-ratio):-2.029418
Number of Samples:2 / 2
Experimental monocyte co-culture study 1 (HS27a)
Bone marrow stromal cell line HS27a cultured with CD14+ monocytes from two different donors.
Control monocyte co-culture study 1 (HS5)
Bone marrow stromal cell line HS5 cultured with CD14+ monocytes from two different donors.

TREM-1 AB study 1 / IgG1 treated monocyte (buffycoat) sample

Relative Expression (log2-ratio):1.9636259
Number of Samples:11 / 11
Experimental TREM-1 AB study 1
Monocytes purified from healthy donor buffycoats were treated with 5 mg/ml TREM-1 (triggering receptor expressed on myeloid cells-1) antibody. RNA was isolated after 2h. ATC code:---
Control IgG1 treated monocyte (buffycoat) sample
Monocytes purified from healthy donor buffycoats were treated with 5 mg/ml isotype-matched murine IgG1 control antibody. RNA was isolated after 2h.

A. fumigatus study 1 / uninfected immature dendritic cell sample

Relative Expression (log2-ratio):1.8667831
Number of Samples:2 / 2
Experimental A. fumigatus study 1
5 x 106 immature dendritic cells were cultivated together with 5 x 106 A. fumigatus germ tubes for 6h until isolation of total RNA.
Control uninfected immature dendritic cell sample
5 x 106 immature dendritic cells were cultivated without fungi.

TGF-ß3 study 1 (early) / BMP-2 study 2 (early)

Relative Expression (log2-ratio):1.8314171
Number of Samples:2 / 3
Experimental TGF-ß3 study 1 (early)
Cultured mesenchymal stem cells from human bone marrow aspirates of healthy donors. Cells were stimulated with 10ng/ml of recombinant TGF-ß3 for 1 day. (Warning: Experiment with gender bias).
Control BMP-2 study 2 (early)
Cultured mesenchymal stem cells from human bone marrow aspirates of healthy donors. Cells were stimulated with 100ng/ml of recombinant BMP-2 for 1 day. (Warning: Experiment with gender bias).

HS-27A / HS-5

Relative Expression (log2-ratio):-1.8060036
Number of Samples:4 / 4
Experimental HS-27A
Human bone marrow stromal cell line derived from a male Caucasian patient. Cells were immortalized by transduction with the human papilloma virus E6/E7 genes. HS-27a supports obblestone area formation by early hematopoietic progenitors. Synonyms:HS27A; HS27a; HS-27a; HS 27a Cellosaurus code:
Control HS-5
Human bone marrow stromal cell line of a 30 yeas old male Caucasian immortalized by transduction with the human papilloma virus E6/E7 genes. HS-5 secretes multiple cytokines that support the proliferation of committed progenitors. Synonyms:HS5; HS 5 Cellosaurus code:

HCC study 20 (CDX; Hep-G2; ectopic) / HCC study 20 (PDX; ectopic)

Relative Expression (log2-ratio):-1.7708321
Number of Samples:3 / 11
Experimental HCC study 20 (CDX; Hep-G2; ectopic)
Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks.
Control HCC study 20 (PDX; ectopic)
Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks.

TGF-ß3 study 1 (intermediate) / BMP-2 study 2 (intermediate)

Relative Expression (log2-ratio):1.6803942
Number of Samples:6 / 6
Experimental TGF-ß3 study 1 (intermediate)
Cultured mesenchymal stem cells from human bone marrow aspirates of healthy donors. Cells were stimulated with 10ng/ml of recombinant TGF-ß3 for 3 days or 7 days. (Warning: Experiment with gender bias).
Control BMP-2 study 2 (intermediate)
Cultured mesenchymal stem cells from human bone marrow aspirates of healthy donors. Cells were stimulated with 100ng/ml of recombinant BMP-2 for 3 days or 7 days. (Warning: Experiment with gender bias).

plasma stimulation study 1 (pneumonia) / healthy plasma treated PBMC sample

Relative Expression (log2-ratio):-1.6793547
Number of Samples:10 / 12
Experimental plasma stimulation study 1 (pneumonia)
Peripheral blood mononuclear cells stimulated for 9 hours with 40% plasma derived from gram-positive pneumonia patients. Bacterial infection was detected in every sample and all patients showed acute illness, a pulmonary infiltrate or consolidation (detected by a radiologist) and at least one of the following symptoms: Body temperature > 37.8 °C or < 36°C, peripheral white blood cell number > 10 000 ul or < 4500 ul or >15% immature neutrophils. Furthermore patients also revealed two of the following three factors: tachypnea, dyspnea or hypoxemia. Samples were collected within the first day of hospitalization.
Control healthy plasma treated PBMC sample
Peripheral blood mononuclear cells treated for 9 hrs with 40% plasma derived from healthy control patients. Patients were free of any infection.