TOP TEN perturbations for 126_s_at (Homo sapiens)

Organism: Homo sapiens
Gene: 126_s_at
Selected probe(set): 206626_x_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 126_s_at (206626_x_at) across 6672 perturbations tested by GENEVESTIGATOR:

HCC study 6 (primary; adrenal gland metastasis) / HCC study 6 (primary; no metastasis)

Relative Expression (log2-ratio):6.0126657
Number of Samples:2 / 10
Experimental HCC study 6 (primary; adrenal gland metastasis)
Liver tissue sample from the primary site of patients with hepatocellular carcinoma (HCC) that metastasized to adrenal gland.
Control HCC study 6 (primary; no metastasis)
Liver tissue sample from the primary site of patients with hepatocellular carcinoma (HCC). Tumor did not metastasize.

male infertility study 1 (mJS5) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):5.2096033
Number of Samples:8 / 7
Experimental male infertility study 1 (mJS5)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-4.634824
Number of Samples:2 / 8
Experimental male infertility study 1 (juvenile; Ad-)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (mJS8) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):4.5389156
Number of Samples:7 / 7
Experimental male infertility study 1 (mJS8)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained many early and elongated but only few mature spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS8.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS7) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):4.4795704
Number of Samples:4 / 7
Experimental male infertility study 1 (mJS7)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained early but no late spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS7.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS2) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-4.431734
Number of Samples:7 / 8
Experimental male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):4.2872763
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

HCC study 20 (CDX; Hep-G2; orthotopic) / HCC study 20 (PDX; orthotopic)

Relative Expression (log2-ratio):-3.6805496
Number of Samples:5 / 11
Experimental HCC study 20 (CDX; Hep-G2; orthotopic)
Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.
Control HCC study 20 (PDX; orthotopic)
Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.

HCC study 3 (area B) / HCC study 3 (area D)

Relative Expression (log2-ratio):3.6009388
Number of Samples:26 / 19
Experimental HCC study 3 (area B)
Liver tissue biopsy sample from patients with HBV-associated HCC (hepatocellular carcinoma) after orthotopic liver transplantation (OLT) or partial hepatectomy. Tumorous sample was taken from the periphery of the tumor (area B). Patients were positive for hepatitis B surface antigen, antibody to hepatitis B core antigen and antibody to hepatitis B antigen and received antiviral treatment with nucleos(t)ide analogues prior to surgery.
Control HCC study 3 (area D)
Liver tissue biopsy sample from patients with HBV-associated HCC (hepatocellular carcinoma) after orthotopic liver transplantation (OLT) or partial hepatectomy. Non-tumorous sample was taken in an area 2-3 cm away from the tumor (area D). Patients were positive for hepatitis B surface antigen, antibody to hepatitis B core antigen and antibody to hepatitis B antigen and received antiviral treatment with nucleos(t)ide analogues prior to surgery.

HCC study 3 (area B) / non-tumor liver tissue

Relative Expression (log2-ratio):3.4983835
Number of Samples:26 / 37
Experimental HCC study 3 (area B)
Liver tissue biopsy sample from patients with HBV-associated HCC (hepatocellular carcinoma) after orthotopic liver transplantation (OLT) or partial hepatectomy. Tumorous sample was taken from the periphery of the tumor (area B). Patients were positive for hepatitis B surface antigen, antibody to hepatitis B core antigen and antibody to hepatitis B antigen and received antiviral treatment with nucleos(t)ide analogues prior to surgery.
Control non-tumor liver tissue
Histologically normal, healthy liver biopsy samples obtained from a clinically unaffected site from patients with HBV-associated HCC (hepatocellular carcinoma) after orthotopic liver transplantation (OLT) or partial hepatectomy. Non-tumorous, non-malignant sample was taken from the unaffected edge of the liver (area E). Patients were positive for hepatitis B surface antigen, antibody to hepatitis B core antigen and antibody to hepatitis B antigen and received antiviral treatment with nucleos(t)ide analogues prior to surgery.

Organism: Homo sapiens
Gene: 126_s_at
Selected probe(set): 208586_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 126_s_at (208586_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

HCC study 20 (CDX; Hep-G2; orthotopic) / HCC study 20 (PDX; orthotopic)

Relative Expression (log2-ratio):-2.034255
Number of Samples:5 / 11
Experimental HCC study 20 (CDX; Hep-G2; orthotopic)
Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.
Control HCC study 20 (PDX; orthotopic)
Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.

HCC study 9 (HBV; HCV) / HCC study 9 (HCV)

Relative Expression (log2-ratio):1.7463093
Number of Samples:2 / 9
Experimental HCC study 9 (HBV; HCV)
Liver tissue biopsy sample from patients with Hepatitis B and C infection related hepatocellular carcinoma (HCC) after resection.
Control HCC study 9 (HCV)
Liver tissue biopsy sample from patients with Hepatitis C infection related hepatocellular carcinoma (HCC) after resection.

HCC study 9 (HBV; HCV) / HCC study 9 (HBV)

Relative Expression (log2-ratio):1.7195044
Number of Samples:2 / 10
Experimental HCC study 9 (HBV; HCV)
Liver tissue biopsy sample from patients with Hepatitis B and C infection related hepatocellular carcinoma (HCC) after resection.
Control HCC study 9 (HBV)
Liver tissue biopsy sample from patients with Hepatitis B infection related hepatocellular carcinoma (HCC) after resection.

HCC study 9 (HBV; HCV) / normal liver tissue

Relative Expression (log2-ratio):1.6884642
Number of Samples:2 / 10
Experimental HCC study 9 (HBV; HCV)
Liver tissue biopsy sample from patients with Hepatitis B and C infection related hepatocellular carcinoma (HCC) after resection.
Control normal liver tissue
Normal, non-tumor liver tissue.

exercise study 33 (YT; slow twitch; pre-exerc.) / exercise study 33 (YUT; slow twitch; pre-exerc.)

Relative Expression (log2-ratio):1.4284153
Number of Samples:5 / 5
Experimental exercise study 33 (YT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from young trained (YT) women (23±2 years old) before the 36th (last) exercise session. Participants underwent 12 weeks of progressive resistance training including 36 training sessions (3 days/week) with three sets of 10 bilateral knee extensions at 70–75% of their one-repetition maximum (1 RM). Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.
Control exercise study 33 (YUT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from young untrained (YUT) women (23±2 years old) before the first exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.

IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C6) / IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C1)

Relative Expression (log2-ratio):-1.3624649
Number of Samples:3 / 3
Experimental IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C6)
Human breast cancer cell line T47D-YA/IRS-2 C6 samples collected 24 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 24 hours.
Control IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C1)
Human breast cancer cell line T47D-YA/IRS-2 C1 samples collected 24 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 24 hours.

IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C6) / IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C1)

Relative Expression (log2-ratio):-1.3523312
Number of Samples:3 / 3
Experimental IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C6)
Human breast cancer cell line T47D-YA/IRS-2 C6 samples collected 4 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 4 hours.
Control IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C1)
Human breast cancer cell line T47D-YA/IRS-2 C1 samples collected 4 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 4 hours.

systemic lupus erythematosus study 3 / normal blood sample

Relative Expression (log2-ratio):1.3068852
Number of Samples:89 / 30
Experimental systemic lupus erythematosus study 3
Blood samples derived from patients with anti-ribonuclear protein-positive (anti-RNP+) systemic lupus erythematosus (SLE).
Control normal blood sample
Blood samples from healthy individuals.

systemic lupus erythematosus study 13 (untreated) / normal PBMC sample

Relative Expression (log2-ratio):-1.1597795
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (untreated)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists.
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

male infertility study 1 (mJS5) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):1.0601368
Number of Samples:8 / 7
Experimental male infertility study 1 (mJS5)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

Organism: Homo sapiens
Gene: 126_s_at
Selected probe(set): 208586_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 126_s_at (208586_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

HCC study 20 (CDX; Hep-G2; orthotopic) / HCC study 20 (PDX; orthotopic)

Relative Expression (log2-ratio):-2.034255
Number of Samples:5 / 11
Experimental HCC study 20 (CDX; Hep-G2; orthotopic)
Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.
Control HCC study 20 (PDX; orthotopic)
Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks.

HCC study 9 (HBV; HCV) / HCC study 9 (HCV)

Relative Expression (log2-ratio):1.7463093
Number of Samples:2 / 9
Experimental HCC study 9 (HBV; HCV)
Liver tissue biopsy sample from patients with Hepatitis B and C infection related hepatocellular carcinoma (HCC) after resection.
Control HCC study 9 (HCV)
Liver tissue biopsy sample from patients with Hepatitis C infection related hepatocellular carcinoma (HCC) after resection.

HCC study 9 (HBV; HCV) / HCC study 9 (HBV)

Relative Expression (log2-ratio):1.7195044
Number of Samples:2 / 10
Experimental HCC study 9 (HBV; HCV)
Liver tissue biopsy sample from patients with Hepatitis B and C infection related hepatocellular carcinoma (HCC) after resection.
Control HCC study 9 (HBV)
Liver tissue biopsy sample from patients with Hepatitis B infection related hepatocellular carcinoma (HCC) after resection.

HCC study 9 (HBV; HCV) / normal liver tissue

Relative Expression (log2-ratio):1.6884642
Number of Samples:2 / 10
Experimental HCC study 9 (HBV; HCV)
Liver tissue biopsy sample from patients with Hepatitis B and C infection related hepatocellular carcinoma (HCC) after resection.
Control normal liver tissue
Normal, non-tumor liver tissue.

exercise study 33 (YT; slow twitch; pre-exerc.) / exercise study 33 (YUT; slow twitch; pre-exerc.)

Relative Expression (log2-ratio):1.4284153
Number of Samples:5 / 5
Experimental exercise study 33 (YT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from young trained (YT) women (23±2 years old) before the 36th (last) exercise session. Participants underwent 12 weeks of progressive resistance training including 36 training sessions (3 days/week) with three sets of 10 bilateral knee extensions at 70–75% of their one-repetition maximum (1 RM). Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.
Control exercise study 33 (YUT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from young untrained (YUT) women (23±2 years old) before the first exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.

IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C6) / IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C1)

Relative Expression (log2-ratio):-1.3624649
Number of Samples:3 / 3
Experimental IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C6)
Human breast cancer cell line T47D-YA/IRS-2 C6 samples collected 24 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 24 hours.
Control IGF-I study 1 (24h; 5nM; T47D-YA/IRS-2 C1)
Human breast cancer cell line T47D-YA/IRS-2 C1 samples collected 24 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 24 hours.

IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C6) / IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C1)

Relative Expression (log2-ratio):-1.3523312
Number of Samples:3 / 3
Experimental IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C6)
Human breast cancer cell line T47D-YA/IRS-2 C6 samples collected 4 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 4 hours.
Control IGF-I study 1 (4h; 5nM; T47D-YA/IRS-2 C1)
Human breast cancer cell line T47D-YA/IRS-2 C1 samples collected 4 hours after treatment with 5 nM IGF-I (insulin-like growth factor I). The cell line was maintained in MEM, 5% fetal bovine serum, penicillin/streptomycin, 1X non-essential amino acids, 6 ng/l insulin, 50 ug/ml G418, and 100 ug/ml hygromycin B. Cells were plated at a density of 3x10exp6 in 150 mm dishes overnight, then the media were replaced by SFM (serum-free media) alone for 24 h prior the treatment. After 24 hours, the cells were treated with SFM plus fibronectin (2 ug/ml) and IGF-I for 4 hours.

systemic lupus erythematosus study 3 / normal blood sample

Relative Expression (log2-ratio):1.3068852
Number of Samples:89 / 30
Experimental systemic lupus erythematosus study 3
Blood samples derived from patients with anti-ribonuclear protein-positive (anti-RNP+) systemic lupus erythematosus (SLE).
Control normal blood sample
Blood samples from healthy individuals.

systemic lupus erythematosus study 13 (untreated) / normal PBMC sample

Relative Expression (log2-ratio):-1.1597795
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (untreated)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists.
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

male infertility study 1 (mJS5) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):1.0601368
Number of Samples:8 / 7
Experimental male infertility study 1 (mJS5)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

Organism: Homo sapiens
Gene: 126_s_at
Selected probe(set): 207493_x_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 126_s_at (207493_x_at) across 6672 perturbations tested by GENEVESTIGATOR:

male infertility study 1 (mJS5) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):5.3026752
Number of Samples:8 / 7
Experimental male infertility study 1 (mJS5)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS8) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):4.741165
Number of Samples:7 / 7
Experimental male infertility study 1 (mJS8)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained many early and elongated but only few mature spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS8.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS7) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):4.574151
Number of Samples:4 / 7
Experimental male infertility study 1 (mJS7)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained early but no late spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS7.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS2) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-4.5565853
Number of Samples:7 / 8
Experimental male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-4.4692106
Number of Samples:2 / 8
Experimental male infertility study 1 (juvenile; Ad-)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; basaloid carcinoma; primary)

Relative Expression (log2-ratio):4.102729
Number of Samples:2 / 3
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; basaloid carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary basaloid carcinoma of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; large cell carcinoma, NOS; primary)

Relative Expression (log2-ratio):4.039154
Number of Samples:2 / 4
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; large cell carcinoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary large cell carcinoma, NOS of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; adenosquamous carcinoma; primary)

Relative Expression (log2-ratio):4.0361013
Number of Samples:2 / 4
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; adenosquamous carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary adenosquamous carcinoma of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; squamous cell carcinoma, NOS; primary) / lung cancer study 1 (PDX; non-small cell carcinoma; primary)

Relative Expression (log2-ratio):-4.020034
Number of Samples:43 / 2
Experimental lung cancer study 1 (PDX; squamous cell carcinoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary squamous cell carcinoma, NOS of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; adenocarcinoma, NOS; primary)

Relative Expression (log2-ratio):3.9836607
Number of Samples:2 / 22
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; adenocarcinoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary adenocarcinoma, NOS of the lung (subcutaneously implanted).

Organism: Homo sapiens
Gene: 126_s_at
Selected probe(set): 207493_x_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 126_s_at (207493_x_at) across 6672 perturbations tested by GENEVESTIGATOR:

male infertility study 1 (mJS5) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):5.3026752
Number of Samples:8 / 7
Experimental male infertility study 1 (mJS5)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS8) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):4.741165
Number of Samples:7 / 7
Experimental male infertility study 1 (mJS8)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained many early and elongated but only few mature spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS8.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS7) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):4.574151
Number of Samples:4 / 7
Experimental male infertility study 1 (mJS7)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained early but no late spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS7.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS2) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-4.5565853
Number of Samples:7 / 8
Experimental male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-4.4692106
Number of Samples:2 / 8
Experimental male infertility study 1 (juvenile; Ad-)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; basaloid carcinoma; primary)

Relative Expression (log2-ratio):4.102729
Number of Samples:2 / 3
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; basaloid carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary basaloid carcinoma of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; large cell carcinoma, NOS; primary)

Relative Expression (log2-ratio):4.039154
Number of Samples:2 / 4
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; large cell carcinoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary large cell carcinoma, NOS of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; adenosquamous carcinoma; primary)

Relative Expression (log2-ratio):4.0361013
Number of Samples:2 / 4
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; adenosquamous carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary adenosquamous carcinoma of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; squamous cell carcinoma, NOS; primary) / lung cancer study 1 (PDX; non-small cell carcinoma; primary)

Relative Expression (log2-ratio):-4.020034
Number of Samples:43 / 2
Experimental lung cancer study 1 (PDX; squamous cell carcinoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary squamous cell carcinoma, NOS of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).

lung cancer study 1 (PDX; non-small cell carcinoma; primary) / lung cancer study 1 (PDX; adenocarcinoma, NOS; primary)

Relative Expression (log2-ratio):3.9836607
Number of Samples:2 / 22
Experimental lung cancer study 1 (PDX; non-small cell carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary non-small cell carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; adenocarcinoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary adenocarcinoma, NOS of the lung (subcutaneously implanted).