TOP TEN perturbations for 1443_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1443_at
Selected probe(set): 205777_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1443_at (205777_at) across 6672 perturbations tested by GENEVESTIGATOR:

renal cell carcinoma study 4 / normal kidney tissue

Relative Expression (log2-ratio):-3.581726
Number of Samples:26 / 3
Experimental renal cell carcinoma study 4
Tumor tissue samples from the kidney of patients with renal cell carcinoma (RCC).
Control normal kidney tissue
Normal adult kidney tissue samples.

renal cell carcinoma study 5 (papillary type) / normal kidney tissue

Relative Expression (log2-ratio):-3.4298792
Number of Samples:19 / 3
Experimental renal cell carcinoma study 5 (papillary type)
Tumor tissue samples from the kidney of patients with papillary renal cell carcinoma (pRCC).
Control normal kidney tissue
Normal adult kidney tissue samples.

HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) / HIF-1a/HIF-2a depletion study 1 (normoxia; AB81)

Relative Expression (log2-ratio):3.4069881
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control HIF-1a/HIF-2a depletion study 1 (normoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with normoxia (20% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

Hep-G2 / HepaRG

Relative Expression (log2-ratio):3.2721825
Number of Samples:9 / 12
Experimental Hep-G2
Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code:
Control HepaRG
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code:

hepatoblastoma study 1 (epithelial mixed) / normal liver tissue

Relative Expression (log2-ratio):3.2354689
Number of Samples:12 / 5
Experimental hepatoblastoma study 1 (epithelial mixed)
Liver tumor tissue samples from children with hepatoblastoma (epithelial type (E-HB); epithelial mixed subtype). Tumors were integrase interactor 1–negative (INI1 or SMARCB1) as recommended by the International ConsensusHBClassification group.
Control normal liver tissue
Normal liver tissue samples.

clear cell renal cell carcinoma study 4 (stage 3) / normal kidney tissue

Relative Expression (log2-ratio):-3.1748915
Number of Samples:14 / 69
Experimental clear cell renal cell carcinoma study 4 (stage 3)
Primary tumor tissue from patients with stage 3 clear cell renal cell carcinoma (ccRCC).
Control normal kidney tissue
Normal kidney tissue samples obtained from healthy individuals.

hepatoblastoma study 1 (NOS) / normal liver tissue

Relative Expression (log2-ratio):3.1711311
Number of Samples:5 / 5
Experimental hepatoblastoma study 1 (NOS)
Liver tumor tissue samples from children with hepatoblastoma (NOS). Tumors were integrase interactor 1–negative (INI1 or SMARCB1) as recommended by the International ConsensusHBClassification group.
Control normal liver tissue
Normal liver tissue samples.

hepatocyte-like cell differentiation study 1 (15d) / hepatocyte-like cell differentiation study 1 (5d)

Relative Expression (log2-ratio):3.1704884
Number of Samples:3 / 3
Experimental hepatocyte-like cell differentiation study 1 (15d)
Hepatocyte-like cells differentiated from human embryonic stem cells (ES, WA09), 15 days after the onset of differentiation. This stage is approximately corresponding to immature hepatocytes. Human H9 (WA09) ES cells were routinely cultured under low oxygen conditions (4% O2/5% CO2) in human ES cell media (DMEM/F12 medium supplemented with 20% knockout serum replacement, non essential amino acids, glutamine, penicillin/streptomycin and bFGF (4ng/ml) on Matrigel coated plates using MEF-conditioned human ES cell media. Differentiation was initiated by culture for 5 days with 100ng/ml Activin A in RPMI/B27 medium under ambient oxygen/5%CO2 followed by 5 days with 20ng/ml BMP4 /10ng/ml FGF-2 in RPMI/B27 under 4%O2/5%CO2, then 5 days with 20ng/ml HGF in RPMI/B27 supplement under 4%O2/5%CO2.
Control hepatocyte-like cell differentiation study 1 (5d)
Hepatocyte-like cell differentiated from human pluripotent, embryonic stem cell line (ES, WA09). ES cells were grown in human ES cell media DMEM/F12 and differentiated to definitive endoderm for 5 days.

clear cell renal cell carcinoma study 4 (stage 2) / normal kidney tissue

Relative Expression (log2-ratio):-3.1584206
Number of Samples:19 / 69
Experimental clear cell renal cell carcinoma study 4 (stage 2)
Primary tumor tissue from patients with stage 2 clear cell renal cell carcinoma (ccRCC).
Control normal kidney tissue
Normal kidney tissue samples obtained from healthy individuals.

hepatocyte-like cell differentiation study 1 (20d; HNF4 depl) / hepatocyte-like cell differentiation study 1 (15d)

Relative Expression (log2-ratio):-3.1218748
Number of Samples:3 / 3
Experimental hepatocyte-like cell differentiation study 1 (20d; HNF4 depl)
Hepatocyte-like cell differentiated from human pluripotent, embryonic stem cell line (ES, WA09) and transfected with shRNA targeting HNF4A. ES cells were grown in human ES cell media DMEM/F12 and differentiated to mature hepatocytes for 20 days. Moreover hESc was stable transfected with lentivirus expressing an shRNA that efficiently depletes HNF4A.
Control hepatocyte-like cell differentiation study 1 (15d)
Hepatocyte-like cells differentiated from human embryonic stem cells (ES, WA09), 15 days after the onset of differentiation. This stage is approximately corresponding to immature hepatocytes. Human H9 (WA09) ES cells were routinely cultured under low oxygen conditions (4% O2/5% CO2) in human ES cell media (DMEM/F12 medium supplemented with 20% knockout serum replacement, non essential amino acids, glutamine, penicillin/streptomycin and bFGF (4ng/ml) on Matrigel coated plates using MEF-conditioned human ES cell media. Differentiation was initiated by culture for 5 days with 100ng/ml Activin A in RPMI/B27 medium under ambient oxygen/5%CO2 followed by 5 days with 20ng/ml BMP4 /10ng/ml FGF-2 in RPMI/B27 under 4%O2/5%CO2, then 5 days with 20ng/ml HGF in RPMI/B27 supplement under 4%O2/5%CO2.