TOP TEN perturbations for 1490_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1490_at
Selected probe(set): 214058_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1490_at (214058_at) across 6672 perturbations tested by GENEVESTIGATOR:

IFN-g study 11 / normal monocyte sample

Relative Expression (log2-ratio):-4.482089
Number of Samples:7 / 7
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

IFN-g study 11 / unstimulated monocyte sample

Relative Expression (log2-ratio):-4.21531
Number of Samples:7 / 11
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control unstimulated monocyte sample
Monocyte samples isolated from healthy donors and incubated in whole blood for 1.5 hour without any stimulation. Following incubation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

stem cell differentiation study 59 (iDRG; 15d) / stem cell differentiation study 59 (8d)

Relative Expression (log2-ratio):-4.1318016
Number of Samples:4 / 4
Experimental stem cell differentiation study 59 (iDRG; 15d)
Immature dorsal root ganglia neurons (iDRGs) obtained by differentiation of WA09 embryonic stem cells. WA09 cells were differentiated for 8 days and subsequently cryopreserved. After thawing, cells were further differentiated for 7 days. Further details are described in the paper.
Control stem cell differentiation study 59 (8d)
WA09 embryonic stem cell samples differentiated for 8 days. Further details are described in the paper.

IFNa-2a study 1 / normal monocyte sample

Relative Expression (log2-ratio):-3.957841
Number of Samples:7 / 7
Experimental IFNa-2a study 1
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml IFNα2a in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

rheumatoid arthritis study 31 / IFN-g study 11

Relative Expression (log2-ratio):3.8167257
Number of Samples:4 / 7
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocytes from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

IFNa-2a study 1 / unstimulated monocyte sample

Relative Expression (log2-ratio):-3.691062
Number of Samples:7 / 11
Experimental IFNa-2a study 1
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml IFNα2a in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control unstimulated monocyte sample
Monocyte samples isolated from healthy donors and incubated in whole blood for 1.5 hour without any stimulation. Following incubation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

pediatric meningococcal sepsis study 1 (0h; monocyte) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-3.6137753
Number of Samples:4 / 3
Experimental pediatric meningococcal sepsis study 1 (0h; monocyte)
Monocyte (CD14+) samples obtained from blood samples of children with (suspected) meningococcal sepsis collected within 0 - 6 hours after admission to the pediatric intensive care unit (PICU). Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.
Control normal monocyte (CD14+) sample
Normal monocyte (CD14+) samples obtained from peripheral blood of healthy children admitted for elective minor non-infectious surgery or MRI. Ten ml of whole blood samples were used to perform a Ficoll density separation using Lymphoprep. Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.

stem cell differentiation study 59 (iDRG; 12d) / stem cell differentiation study 59 (8d)

Relative Expression (log2-ratio):-3.5178785
Number of Samples:4 / 4
Experimental stem cell differentiation study 59 (iDRG; 12d)
Immature dorsal root ganglia neurons (iDRGs) obtained by differentiation of WA09 embryonic stem cells. WA09 cells were differentiated for 8 days and subsequently cryopreserved. After thawing, cells were further differentiated for 4 days. Further details are described in the paper.
Control stem cell differentiation study 59 (8d)
WA09 embryonic stem cell samples differentiated for 8 days. Further details are described in the paper.

monocyte activation study 1 (TLR/1L; 24h) / monocyte activation study 1 (TLR/1L; 6h)

Relative Expression (log2-ratio):3.4480324
Number of Samples:5 / 5
Experimental monocyte activation study 1 (TLR/1L; 24h)
Monocytes were isolated from five healthy donors, cultured in RPMI with 10% vitamin D–sufficient (100 nM) human serum and activated for 24 hours with 1 ug/ml mycobacterial 19-kDa triacylated lipopeptide (TLR2/1 ligand, TLR2/1L). This type of activation is via heterodimeric Toll-like receptor 2 and Toll-like receptor 1 (TLR2/1).
Control monocyte activation study 1 (TLR/1L; 6h)
Monocytes were isolated from five healthy donors, cultured in RPMI with 10% vitamin D–sufficient (100 nM) human serum and activated for 6 hours with 1 ug/ml mycobacterial 19-kDa triacylated lipopeptide (TLR2/1 ligand, TLR2/1L). This type of activation is via heterodimeric Toll-like receptor 2 and Toll-like receptor 1 (TLR2/1).

diclofenac study 6 (55uM) / vehicle (PBS) treated HepG2 sample

Relative Expression (log2-ratio):3.4407892
Number of Samples:3 / 12
Experimental diclofenac study 6 (55uM)
HepG2 cells treated with compound: diclofenac (55uM; CAS no.:15307-86-5) for 24 hours. Diclofenac is hepatotoxic and may cause necrosis. HepG2 cells were treated with the IC20 concentration measured after 24 hours. ATC code:, ,
Control vehicle (PBS) treated HepG2 sample
HepG2 cells treated with PBS (0.5% v/v) as solvent control for 24 hours.