TOP TEN perturbations for 1503_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1503_at
Selected probe(set): 214727_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1503_at (214727_at) across 6672 perturbations tested by GENEVESTIGATOR:

nutlin-3 study 2 (control vector; 10uM) / vehicle treated MCF-7-con cell sample

Relative Expression (log2-ratio):-4.084482
Number of Samples:6 / 3
Experimental nutlin-3 study 2 (control vector; 10uM)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 48 hours. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---
Control vehicle treated MCF-7-con cell sample
Human breast adenocarcinoma cells MCF-7 expressing wild type p53 stably transfected with control empty vector pSUPER, vehicle treated with DMSO (0.1%) for 48 hours. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin.

nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector) / flagellin study 4 (control vector; 500 ng/ml)

Relative Expression (log2-ratio):-3.9090462
Number of Samples:6 / 2
Experimental nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 45 hours followed by an additional 3 hours treatment of nutlin-3 (10uM) and flagellin (500 ng/ml). Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---
Control flagellin study 4 (control vector; 500 ng/ml)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with DMSO 0.1% for 45 hours followed by an additional 3 hours with flagellin (500 ng/ml). Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin.

dengue fever study 10 (DHF) / normal naive CD8 T cell sample

Relative Expression (log2-ratio):3.9016657
Number of Samples:2 / 5
Experimental dengue fever study 10 (DHF)
Activated CD8 T cells derived from peripheral blood mononuclear cells (PBMCs) of Thai individuals with Dengue hemorrhagic fever (DF) characterized by the WHO 1997. FACS-sorted CD3+, CD8+, HLA_DR+, and CD38+ effector CD8 T subtype cells were used for analysis.
Control normal naive CD8 T cell sample
Normal naive CD8 T cells derived from peripheral blood mononuclear cells (PBMCs) of healthy Thai individuals. FACS-sorted CD3+, CD8+, CD45RA+, and CCR7+ naive CD8 T subtype cells were used for analysis.

nutlin-3 study 2 (p53 shRNA;10uM) / nutlin-3 study 2 (control vector; 10uM)

Relative Expression (log2-ratio):3.6581192
Number of Samples:3 / 6
Experimental nutlin-3 study 2 (p53 shRNA;10uM)
Human breast adenocarcinoma cells MCF-7 with depleted p53 (shRNA), treated with nutlin-3 (10 uM) for 48 hours. Cells were stably transfected with vector pSUPER and expressing shRNA to p53. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---
Control nutlin-3 study 2 (control vector; 10uM)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 48 hours. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---

sangivamycin study 1 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):-3.5849705
Number of Samples:2 / 2
Experimental sangivamycin study 1
MCF7 cells treated with 2uM sangivamycin for 24 hours. ATC code:---
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

C. difficile study 1 (TcdA; 24h) / untreated HCT 8 cell sample

Relative Expression (log2-ratio):-3.4569702
Number of Samples:2 / 2
Experimental C. difficile study 1 (TcdA; 24h)
HCT 8 cells were treated with 100 ng/ml Toxin A (TcdA) isolated from Clostridium difficile strain VPI-10643 in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum and 1mM sodium pyruvate for 24 hours.
Control untreated HCT 8 cell sample
Untreated HCT 8 cell sample cultured in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum and 1mM sodium pyruvate for 24 hours.

nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (p53 shRNA) / nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector)

Relative Expression (log2-ratio):3.3013353
Number of Samples:3 / 6
Experimental nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (p53 shRNA)
Human breast adenocarcinoma cells MCF-7 with depleted p53 (shRNA), treated with nutlin-3 (10 uM) for 45 hours followed by an additional 3 hours treatment of nutlin-3 (10 uM) and flagellin (500 ng/ml). Cells were stably transfected with vector pSUPER and expressing shRNA to p53. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---
Control nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 45 hours followed by an additional 3 hours treatment of nutlin-3 (10uM) and flagellin (500 ng/ml). Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---

C. difficile study 1 (TcdB; 24h) / untreated HCT 8 cell sample

Relative Expression (log2-ratio):-3.2329826
Number of Samples:2 / 2
Experimental C. difficile study 1 (TcdB; 24h)
HCT 8 cells were treated with 100 ng/ml Toxin B (TcdB) isolated from Clostridium difficile strain VPI-10643 in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum and 1mM sodium pyruvate for 24 hours.
Control untreated HCT 8 cell sample
Untreated HCT 8 cell sample cultured in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum and 1mM sodium pyruvate for 24 hours.

doxorubicin study 3 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):-3.1571426
Number of Samples:2 / 2
Experimental doxorubicin study 3
MCF7 cells treated with 2uM doxorubicin for 24 hours. ATC code:
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):3.0476127
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.