TOP TEN perturbations for 1517_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1517_at
Selected probe(set): 207913_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1517_at (207913_at) across 6672 perturbations tested by GENEVESTIGATOR:

mucociliary differentiation study 1 (late) / ALI-cultured bronchial epithelial cell sample

Relative Expression (log2-ratio):6.0083904
Number of Samples:8 / 3
Experimental mucociliary differentiation study 1 (late)
Primary human bronchial epithelial cells (HBECs) harvested at day 17, 21 and 28 of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).
Control ALI-cultured bronchial epithelial cell sample
Primary human bronchial epithelial cells (HBECs) harvested at day 0, the first day of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).

mucociliary differentiation study 1 (intermediate) / ALI-cultured bronchial epithelial cell sample

Relative Expression (log2-ratio):5.361453
Number of Samples:11 / 3
Experimental mucociliary differentiation study 1 (intermediate)
Primary human bronchial epithelial cells (HBECs) harvested at day 8, 10, 12 and 14 of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).
Control ALI-cultured bronchial epithelial cell sample
Primary human bronchial epithelial cells (HBECs) harvested at day 0, the first day of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):4.873733
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):4.856769
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

smoking study 83 (non-smoker) / untreated lung epithelial culture sample (non-smoker)

Relative Expression (log2-ratio):-3.8279295
Number of Samples:2 / 2
Experimental smoking study 83 (non-smoker)
Air-liquid interface lung epithelial culture exposed to cigarette smoke (30 mins exposure on four separate days). Lung epithelial cells were isolated from non-smokers and cultures were placed in smoking chambers with basal surface in contact with media. They were continually exposed to smoke (from filtered Kentucky reference (3R4F) cigarettes) for 30mins on days 1, 2, 5 and 6 (smoke/air dilution of 1:10 or 1:20).
Control untreated lung epithelial culture sample (non-smoker)
Untreated air-liquid interface lung epithelial culture. Lung epithelial cells were isolated from non-smokers and cultures were maintained in proprietary media.

smoking study 65 (24h) / normal sham-exposed bronchial organotypic tissue

Relative Expression (log2-ratio):-2.5046434
Number of Samples:3 / 3
Experimental smoking study 65 (24h)
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 24 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed bronchial organotypic tissue
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 24 hours. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

smoking study 65 (48h) / normal sham-exposed bronchial organotypic tissue

Relative Expression (log2-ratio):-2.268489
Number of Samples:3 / 3
Experimental smoking study 65 (48h)
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 48 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed bronchial organotypic tissue
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 48 hours. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l) / air exposed organotypic small airway epithelium culture sample (24h; 3R4F)

Relative Expression (log2-ratio):-2.2464647
Number of Samples:5 / 5
Experimental smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 24 hours after smoke exposure the cells were collected for RNA extraction.
Control air exposed organotypic small airway epithelium culture sample (24h; 3R4F)
Organotypic human small airway epithelium culture 24 hours after exposure to conditioned fresh air. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for 3R4F smoke exposure at timepoint 24 hours after exposure.

smoking study 80 (small airway epith; CHTP1.2; 24h; 0.15mg/l) / smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)

Relative Expression (log2-ratio):2.21496
Number of Samples:6 / 5
Experimental smoking study 80 (small airway epith; CHTP1.2; 24h; 0.15mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to 0.15 mg nicotine/l aerosol from a potential modified-risk tobacco product, carbon-heated tobacco product 1.2 (CHTP1.2). Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The aerosol was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each CHTP1.2 stick was used for a total of 12 puffs per stick. The cultures were exposed to aerosol continuously for 28 minutes in smoking machine and 24 hours after aerosol exposure the cells were collected for RNA extraction.
Control smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 24 hours after smoke exposure the cells were collected for RNA extraction.

trachoma study 1 (C. trachomatis positive) / normal tarsal conjunctiva tissue

Relative Expression (log2-ratio):-2.1669416
Number of Samples:18 / 20
Experimental trachoma study 1 (C. trachomatis positive)
Swabs from the tarsal conjunctiva of patients with clinical signs of acute trachoma and infection with Chlamydia trachomatis.
Control normal tarsal conjunctiva tissue
Swabs from the tarsal conjunctiva of subjects without clinical signs of active trachoma in both eyes and absence of C. trachomatis infection.

Organism: Homo sapiens
Gene: 1517_at
Selected probe(set): 207913_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1517_at (207913_at) across 6672 perturbations tested by GENEVESTIGATOR:

mucociliary differentiation study 1 (late) / ALI-cultured bronchial epithelial cell sample

Relative Expression (log2-ratio):6.0083904
Number of Samples:8 / 3
Experimental mucociliary differentiation study 1 (late)
Primary human bronchial epithelial cells (HBECs) harvested at day 17, 21 and 28 of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).
Control ALI-cultured bronchial epithelial cell sample
Primary human bronchial epithelial cells (HBECs) harvested at day 0, the first day of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).

mucociliary differentiation study 1 (intermediate) / ALI-cultured bronchial epithelial cell sample

Relative Expression (log2-ratio):5.361453
Number of Samples:11 / 3
Experimental mucociliary differentiation study 1 (intermediate)
Primary human bronchial epithelial cells (HBECs) harvested at day 8, 10, 12 and 14 of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).
Control ALI-cultured bronchial epithelial cell sample
Primary human bronchial epithelial cells (HBECs) harvested at day 0, the first day of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells).

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):4.873733
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):4.856769
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

smoking study 83 (non-smoker) / untreated lung epithelial culture sample (non-smoker)

Relative Expression (log2-ratio):-3.8279295
Number of Samples:2 / 2
Experimental smoking study 83 (non-smoker)
Air-liquid interface lung epithelial culture exposed to cigarette smoke (30 mins exposure on four separate days). Lung epithelial cells were isolated from non-smokers and cultures were placed in smoking chambers with basal surface in contact with media. They were continually exposed to smoke (from filtered Kentucky reference (3R4F) cigarettes) for 30mins on days 1, 2, 5 and 6 (smoke/air dilution of 1:10 or 1:20).
Control untreated lung epithelial culture sample (non-smoker)
Untreated air-liquid interface lung epithelial culture. Lung epithelial cells were isolated from non-smokers and cultures were maintained in proprietary media.

smoking study 65 (24h) / normal sham-exposed bronchial organotypic tissue

Relative Expression (log2-ratio):-2.5046434
Number of Samples:3 / 3
Experimental smoking study 65 (24h)
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 24 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed bronchial organotypic tissue
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 24 hours. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

smoking study 65 (48h) / normal sham-exposed bronchial organotypic tissue

Relative Expression (log2-ratio):-2.268489
Number of Samples:3 / 3
Experimental smoking study 65 (48h)
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 48 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed bronchial organotypic tissue
Human bronchial organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 48 hours. Organotypic culture consisted in a co-culture of primary bronchial respiratory epithelial cells derived from a healthy nonsmoking 67-year-old Caucasian female and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l) / air exposed organotypic small airway epithelium culture sample (24h; 3R4F)

Relative Expression (log2-ratio):-2.2464647
Number of Samples:5 / 5
Experimental smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 24 hours after smoke exposure the cells were collected for RNA extraction.
Control air exposed organotypic small airway epithelium culture sample (24h; 3R4F)
Organotypic human small airway epithelium culture 24 hours after exposure to conditioned fresh air. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for 3R4F smoke exposure at timepoint 24 hours after exposure.

smoking study 80 (small airway epith; CHTP1.2; 24h; 0.15mg/l) / smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)

Relative Expression (log2-ratio):2.21496
Number of Samples:6 / 5
Experimental smoking study 80 (small airway epith; CHTP1.2; 24h; 0.15mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to 0.15 mg nicotine/l aerosol from a potential modified-risk tobacco product, carbon-heated tobacco product 1.2 (CHTP1.2). Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The aerosol was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each CHTP1.2 stick was used for a total of 12 puffs per stick. The cultures were exposed to aerosol continuously for 28 minutes in smoking machine and 24 hours after aerosol exposure the cells were collected for RNA extraction.
Control smoking study 80 (small airway epith; 3R4F; 24h; 0.16mg/l)
Organotypic human small airway epithelium culture 24 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 24 hours after smoke exposure the cells were collected for RNA extraction.

trachoma study 1 (C. trachomatis positive) / normal tarsal conjunctiva tissue

Relative Expression (log2-ratio):-2.1669416
Number of Samples:18 / 20
Experimental trachoma study 1 (C. trachomatis positive)
Swabs from the tarsal conjunctiva of patients with clinical signs of acute trachoma and infection with Chlamydia trachomatis.
Control normal tarsal conjunctiva tissue
Swabs from the tarsal conjunctiva of subjects without clinical signs of active trachoma in both eyes and absence of C. trachomatis infection.