TOP TEN perturbations for 1552349_a_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1552349_a_at
Selected probe(set): 1552348_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1552349_a_at (1552348_at) across 6622 perturbations tested by GENEVESTIGATOR:

systemic lupus erythematosus study 13 (pioglitazone) / pioglitazone study 4 (1uM; 6h)

Relative Expression (log2-ratio):3.3861399
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (pioglitazone)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients and cultured with pioglitazone. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists. ATC code:
Control pioglitazone study 4 (1uM; 6h)
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects and cultured with pioglitazone. Freshly isolated PBMCs were cultured in RPMI/10% FBS in the presence of 1uM pioglitazone for 6 hours. ATC code:

ovarian tumor study 27 (papillary serous cystadenocarcinoma; metastase) / ovarian tumor study 27 (endometrioid carcinoma; metastase)

Relative Expression (log2-ratio):-3.3237286
Number of Samples:53 / 4
Experimental ovarian tumor study 27 (papillary serous cystadenocarcinoma; metastase)
Metastatic tumor tissue samples obtained from different anatomical sites (colon, omentum, peritoneum and unstated sites) of female patients with primary papillary serous cystadenocarcinoma of the ovary.
Control ovarian tumor study 27 (endometrioid carcinoma; metastase)
Metastatic tumor tissue samples obtained from the peritoneum and other unstated sample site of female patients with primary endometrioid carcinoma of the ovary.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):3.2725716
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

ovarian tumor study 27 (endometrioid carcinoma; metastase) / ovarian tumor study 27 (endometrioid carcinoma; primary)

Relative Expression (log2-ratio):3.2310228
Number of Samples:4 / 16
Experimental ovarian tumor study 27 (endometrioid carcinoma; metastase)
Metastatic tumor tissue samples obtained from the peritoneum and other unstated sample site of female patients with primary endometrioid carcinoma of the ovary.
Control ovarian tumor study 27 (endometrioid carcinoma; primary)
Primary tumor tissue samples obtained from the ovary of female patients with endometrioid carcinoma.

asthma study 22 (mixed granulocytic AIP) / asthma study 22 (paucigranulocytic AIP)

Relative Expression (log2-ratio):3.0282488
Number of Samples:5 / 10
Experimental asthma study 22 (mixed granulocytic AIP)
Sputum samples from asthmatic patients with mixed granulocytic airway inflammatory phenotype (AIP).
Control asthma study 22 (paucigranulocytic AIP)
Sputum samples from asthmatic patients with paucigranulocytic airway inflammatory phenotype (AIP).

LOX1 overexpr. study 1 (adenovirus, 0h) / unstimulated HAECT cell sample

Relative Expression (log2-ratio):-2.977273
Number of Samples:3 / 3
Experimental LOX1 overexpr. study 1 (adenovirus, 0h)
Untreated (0h) immortalized human aortic endothelial cell line (HAECT) overexpressing LOX1. LOX1 overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).
Control unstimulated HAECT cell sample
Untreated (0h) immortalized human aortic endothelial cell line (HAECT) overexpressing GFP (control). GFP overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).

oxLDL study 1 (6h; LOX1) / oxLDL study 1 (6h; GFP)

Relative Expression (log2-ratio):-2.9097948
Number of Samples:3 / 3
Experimental oxLDL study 1 (6h; LOX1)
Immortalized human aortic endothelial cell line (HAECT) overexpressing LOX1 and treated for 6 hours with 50ug/ml oxidized low-density lipoprotein (oxLDL). LOX1 overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).
Control oxLDL study 1 (6h; GFP)
Immortalized human aortic endothelial cell line (HAECT) overexpressing GFP and treated for 6 hours with 50ug/ml oxidized low-density lipoprotein (oxLDL). GFP overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).

rectal cancer study 2 (post; no therapy) / rectal cancer study 2 (pre; no therapy)

Relative Expression (log2-ratio):2.8803234
Number of Samples:2 / 2
Experimental rectal cancer study 2 (post; no therapy)
Tumor tissue samples obtained during a surgical resection from the rectum of untreated (no pre-operative treated) patients with rectal adenocarcinoma.Tumor stage was assigned according to TMN classification of UICC 6th edition 2002.
Control rectal cancer study 2 (pre; no therapy)
Tumor tissue samples obtained during a diagnostic biopsy from the rectum of patients with rectal adenocarcinoma. Tumor stage was assigned according to TMN classification of UICC 6th edition 2002.

oxLDL study 1 (12h; LOX1) / oxLDL study 1 (12h; GFP)

Relative Expression (log2-ratio):-2.7920027
Number of Samples:3 / 3
Experimental oxLDL study 1 (12h; LOX1)
Immortalized human aortic endothelial cell line (HAECT) overexpressing LOX1 and treated for 12 hours with 50ug/ml oxidized low-density lipoprotein (oxLDL). LOX1 overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).
Control oxLDL study 1 (12h; GFP)
Immortalized human aortic endothelial cell line (HAECT) overexpressing GFP and treated for 12 hours with 50ug/ml oxidized low-density lipoprotein (oxLDL). GFP overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).

oxLDL study 1 (24h; LOX1) / oxLDL study 1 (24h; GFP)

Relative Expression (log2-ratio):-2.7691708
Number of Samples:3 / 3
Experimental oxLDL study 1 (24h; LOX1)
Immortalized human aortic endothelial cell line (HAECT) overexpressing LOX1 and treated for 24 hours with 50ug/ml oxidized low-density lipoprotein (oxLDL). LOX1 overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).
Control oxLDL study 1 (24h; GFP)
Immortalized human aortic endothelial cell line (HAECT) overexpressing GFP and treated for 24 hours with 50ug/ml oxidized low-density lipoprotein (oxLDL). GFP overexpression was generated by adenoviral infection (MOI 150). Cells were cultured in EGM-2 medium and maintained at 34 °C (to allow active large-T antigen expression).