TOP TEN perturbations for 1552450_a_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1552450_a_at
Selected probe(set): 1552450_a_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1552450_a_at (1552450_a_at) across 6672 perturbations tested by GENEVESTIGATOR:

male infertility study 1 (juvenile; Ad+) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-2.9010258
Number of Samples:5 / 8
Experimental male infertility study 1 (juvenile; Ad+)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained typical level of A-dark (Ad+) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (mJS3) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-2.882678
Number of Samples:3 / 8
Experimental male infertility study 1 (mJS3)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained Sertoli cells but rarely spermatogonia. Tissue samples were classified based on modified Johnsen score (mJS) as mJS3.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (mJS2) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-2.817727
Number of Samples:7 / 8
Experimental male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-2.7782688
Number of Samples:2 / 8
Experimental male infertility study 1 (juvenile; Ad-)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (mJS8) / male infertility study 1 (mJS3)

Relative Expression (log2-ratio):2.3311644
Number of Samples:7 / 3
Experimental male infertility study 1 (mJS8)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained many early and elongated but only few mature spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS8.
Control male infertility study 1 (mJS3)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained Sertoli cells but rarely spermatogonia. Tissue samples were classified based on modified Johnsen score (mJS) as mJS3.

male infertility study 1 (mJS8) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):2.2662134
Number of Samples:7 / 7
Experimental male infertility study 1 (mJS8)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained many early and elongated but only few mature spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS8.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

male infertility study 1 (mJS7) / male infertility study 1 (mJS3)

Relative Expression (log2-ratio):1.9380655
Number of Samples:4 / 3
Experimental male infertility study 1 (mJS7)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained early but no late spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS7.
Control male infertility study 1 (mJS3)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained Sertoli cells but rarely spermatogonia. Tissue samples were classified based on modified Johnsen score (mJS) as mJS3.

male infertility study 1 (mJS7) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):1.8731146
Number of Samples:4 / 7
Experimental male infertility study 1 (mJS7)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained early but no late spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS7.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

stem cell differentiation study 50 (IRF2 shRNA; ASC; proerythroblast) / stem cell differentiation study 50 (mock shRNA; ASC; proerythroblast)

Relative Expression (log2-ratio):-1.583487
Number of Samples:3 / 4
Experimental stem cell differentiation study 50 (IRF2 shRNA; ASC; proerythroblast)
Proerythroblast differentiated from IRF2 (interferon regulatory factor 2) shRNA transduced hematopoietic stem/progenitor cells (CD34+). CD34+ hematopoietic stem/progenitor cells (HSC) were transduced with lentivirus expressing shRNA targeting IRF2 gene with following differentiation to the erythroid lineage. HSCs were obtained from magnetically sorted G-CSF mobilized peripheral blood mononuclear cells of healthy donors. Cells were expanded in StemSpan SFEM medium supplied with Flt-3 ligand (100 ng/ml), SCF (100 ng/ml), IL-3 (20 ng/ml), IL-6 (20 ng/ml) and penicillin/streptomycin. Cells were harvested for total RNA extraction at the proerythroblast stage of differentiation.
Control stem cell differentiation study 50 (mock shRNA; ASC; proerythroblast)
Proerythroblast differentiated from control shRNA transduced hematopoietic stem/progenitor cells (CD34+). CD34+ hematopoietic stem/progenitor cells (HSC) were transduced with lentivirus expressing control shRNA with following differentiation to the erythroid lineage. HSCs were obtained from magnetically sorted G-CSF mobilized peripheral blood mononuclear cells of healthy donors. Cells were expanded in StemSpan SFEM medium supplied with Flt-3 ligand (100 ng/ml), SCF (100 ng/ml), IL-3 (20 ng/ml), IL-6 (20 ng/ml) and penicillin/streptomycin. Cells were harvested for total RNA extraction at the proerythroblast stage of differentiation.

stem cell differentiation study 50 (IRF6 shRNA; ASC; proerythroblast) / stem cell differentiation study 50 (mock shRNA; ASC; proerythroblast)

Relative Expression (log2-ratio):-1.5535316
Number of Samples:3 / 4
Experimental stem cell differentiation study 50 (IRF6 shRNA; ASC; proerythroblast)
Proerythroblast differentiated from IRF6 (interferon regulatory factor 6) shRNA transduced hematopoietic stem/progenitor cells (CD34+). CD34+ hematopoietic stem/progenitor cells (HSC) were transduced with lentivirus expressing shRNA targeting IRF6 gene with following differentiation to the erythroid lineage. HSCs were obtained from magnetically sorted G-CSF mobilized peripheral blood mononuclear cells of healthy donors. Cells were expanded in StemSpan SFEM medium supplied with Flt-3 ligand (100 ng/ml), SCF (100 ng/ml), IL-3 (20 ng/ml), IL-6 (20 ng/ml) and penicillin/streptomycin. Cells were harvested for total RNA extraction at the proerythroblast stage of differentiation.
Control stem cell differentiation study 50 (mock shRNA; ASC; proerythroblast)
Proerythroblast differentiated from control shRNA transduced hematopoietic stem/progenitor cells (CD34+). CD34+ hematopoietic stem/progenitor cells (HSC) were transduced with lentivirus expressing control shRNA with following differentiation to the erythroid lineage. HSCs were obtained from magnetically sorted G-CSF mobilized peripheral blood mononuclear cells of healthy donors. Cells were expanded in StemSpan SFEM medium supplied with Flt-3 ligand (100 ng/ml), SCF (100 ng/ml), IL-3 (20 ng/ml), IL-6 (20 ng/ml) and penicillin/streptomycin. Cells were harvested for total RNA extraction at the proerythroblast stage of differentiation.