TOP TEN perturbations for 1552501_a_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1552501_a_at
Selected probe(set): 1552501_a_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1552501_a_at (1552501_a_at) across 6672 perturbations tested by GENEVESTIGATOR:

pediatric meningococcal sepsis study 1 (24h; monocyte) / pediatric meningococcal sepsis study 1 (0h; monocyte)

Relative Expression (log2-ratio):2.8601732
Number of Samples:3 / 4
Experimental pediatric meningococcal sepsis study 1 (24h; monocyte)
Monocyte (CD14+) samples obtained from blood samples of children with (suspected) meningococcal sepsis collected 24 hours after admission to the pediatric intensive care unit (PICU). Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.
Control pediatric meningococcal sepsis study 1 (0h; monocyte)
Monocyte (CD14+) samples obtained from blood samples of children with (suspected) meningococcal sepsis collected within 0 - 6 hours after admission to the pediatric intensive care unit (PICU). Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.

pediatric meningococcal sepsis study 1 (0h; monocyte) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-2.7958508
Number of Samples:4 / 3
Experimental pediatric meningococcal sepsis study 1 (0h; monocyte)
Monocyte (CD14+) samples obtained from blood samples of children with (suspected) meningococcal sepsis collected within 0 - 6 hours after admission to the pediatric intensive care unit (PICU). Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.
Control normal monocyte (CD14+) sample
Normal monocyte (CD14+) samples obtained from peripheral blood of healthy children admitted for elective minor non-infectious surgery or MRI. Ten ml of whole blood samples were used to perform a Ficoll density separation using Lymphoprep. Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.

uterine/pelvic pathology study 2 (mid-se MCP) / uterine/pelvic pathology study 2 (late se MCP)

Relative Expression (log2-ratio):2.2937183
Number of Samples:14 / 2
Experimental uterine/pelvic pathology study 2 (mid-se MCP)
Endometrial tissue samples collected from women with uterine/pelvic pathology in the mid-secretory menstrual cycle phase (MCP). The group contained cycling women 20 – 50 years old with symptomatic uterine fibroids, pelvic organ prolapse, and adenomyosis. The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis. Patients with hormonal treatment within previous 3 months and presence of malignancy or major systemic disease were excluded.
Control uterine/pelvic pathology study 2 (late se MCP)
Endometrial tissue samples collected from women with uterine/pelvic pathology in the late secretory menstrual cycle phase (MCP). The group contained cycling women 20 – 50 years old with symptomatic uterine fibroids, pelvic organ prolapse, and adenomyosis. The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis. Patients with hormonal treatment within previous 3 months and presence of malignancy or major systemic disease were excluded.

TNF-ɑ study 20 / normal monocyte sample

Relative Expression (log2-ratio):-1.9106188
Number of Samples:3 / 7
Experimental TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

alopecia areata study 1 (AT; lesional; validation) / normal skin tissue (validation)

Relative Expression (log2-ratio):1.8952513
Number of Samples:3 / 13
Experimental alopecia areata study 1 (AT; lesional; validation)
Lesional scalp skin punch biopsies obtained from patients who suffered from alopecia areata totalis (AT) assigned to validation dataset.
Control normal skin tissue (validation)
Scalp skin punch biopsies obtained from healthy donors assigned to validation dataset.

IL-4; GM-CSF study 1 (intermediate) / untreated monocyte sample

Relative Expression (log2-ratio):-1.7246265
Number of Samples:7 / 12
Experimental IL-4; GM-CSF study 1 (intermediate)
Monocytes, cultured with vehicle (DMSO/ethanol) and 500 U/ml IL-4 and 800 U/ml GM-CSF for 24 hours.
Control untreated monocyte sample
Freshly isolated human monocytes from healthy donors.

TNF-ɑ study 20 / unstimulated monocyte sample

Relative Expression (log2-ratio):-1.587636
Number of Samples:3 / 11
Experimental TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control unstimulated monocyte sample
Monocyte samples isolated from healthy donors and incubated in whole blood for 1.5 hour without any stimulation. Following incubation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

IL-4; GM-CSF study 1 (late) / untreated monocyte sample

Relative Expression (log2-ratio):-1.5869608
Number of Samples:6 / 12
Experimental IL-4; GM-CSF study 1 (late)
Monocytes, cultured with vehicle (DMSO/ethanol) and 500 U/ml IL-4 and 800 U/ml GM-CSF for 5 days. Cytokine treatment was repeated at day 3.
Control untreated monocyte sample
Freshly isolated human monocytes from healthy donors.

alopecia areata study 1 (AAP; lesional; validation) / alopecia areata study 1 (AT; lesional; validation)

Relative Expression (log2-ratio):-1.5533037
Number of Samples:8 / 3
Experimental alopecia areata study 1 (AAP; lesional; validation)
Lesional scalp skin punch biopsies obtained from patients who suffered from alopecia areata patchy-type (AAP) assigned to validation dataset. Several patients had transient patchy type of AAP with dissease duration less than 1 year.
Control alopecia areata study 1 (AT; lesional; validation)
Lesional scalp skin punch biopsies obtained from patients who suffered from alopecia areata totalis (AT) assigned to validation dataset.

uterine/pelvic pathology study 2 (pro. MCP) / uterine/pelvic pathology study 2 (mid-se MCP)

Relative Expression (log2-ratio):-1.5108299
Number of Samples:15 / 14
Experimental uterine/pelvic pathology study 2 (pro. MCP)
Endometrial tissue samples collected from women with uterine/pelvic pathology in the proliferative menstrual cycle phase (MCP). The group contained cycling women 20 – 50 years old with symptomatic uterine fibroids, pelvic organ prolapse, and adenomyosis. The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis. Patients with hormonal treatment within previous 3 months and presence of malignancy or major systemic disease were excluded.
Control uterine/pelvic pathology study 2 (mid-se MCP)
Endometrial tissue samples collected from women with uterine/pelvic pathology in the mid-secretory menstrual cycle phase (MCP). The group contained cycling women 20 – 50 years old with symptomatic uterine fibroids, pelvic organ prolapse, and adenomyosis. The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis. Patients with hormonal treatment within previous 3 months and presence of malignancy or major systemic disease were excluded.