TOP TEN perturbations for 1552822_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1552822_at
Selected probe(set): 225302_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1552822_at (225302_at) across 6673 perturbations tested by GENEVESTIGATOR:

brefeldin A study 1 (0.5ug/ml; p53HCT116) / untreated p53HCT116 cell sample

Relative Expression (log2-ratio):4.3138027
Number of Samples:2 / 3
Experimental brefeldin A study 1 (0.5ug/ml; p53HCT116)
Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated p53HCT116 cell sample
Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample

Relative Expression (log2-ratio):3.6493025
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116)
Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 cell sample
Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

lactic acid study 1 (hypoxia) / untreated breast epithelial cell sample

Relative Expression (log2-ratio):-2.554946
Number of Samples:3 / 3
Experimental lactic acid study 1 (hypoxia)
Breast epithelial cells exposed to 25mM lactic acid of pH6.7 and 2% hypoxia. ATC code:
Control untreated breast epithelial cell sample
Breast epithelial cells in control media under normoxia.

tunicamycin study 2 (2ug/ml; HCT 116 DICER1(-/-)) / untreated HCT 116 DICER1(-/-) cell sample

Relative Expression (log2-ratio):2.518815
Number of Samples:3 / 3
Experimental tunicamycin study 2 (2ug/ml; HCT 116 DICER1(-/-))
Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 was treated with 2 ug/ml tunicamycin for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 DICER1(-/-) cell sample
Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 by was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

rheumatoid arthritis study 31 / TNF-ɑ study 20

Relative Expression (log2-ratio):2.3944502
Number of Samples:4 / 3
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocytes from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

F. tularensis study 1 (tularensis Schu S4) / uninfected peripheral blood monocyte sample

Relative Expression (log2-ratio):-2.3656874
Number of Samples:4 / 6
Experimental F. tularensis study 1 (tularensis Schu S4)
Peripheral blood monocytes infected with the Schu S4 isolate of Francisella tularensis (100 MOI) for 24 hours.
Control uninfected peripheral blood monocyte sample
Peripheral blood monocytes uninfected.

ovarian tumor study 16 / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):-2.3416662
Number of Samples:3 / 5
Experimental ovarian tumor study 16
Human epithelial tumor cell samples from the ovary of patients with papillary serous carcinoma. Samples were derived by laser capture microdissection (LCM).
Control normal ovarian surface epithelial cell sample
Human epithelial cell samples from histopathological normal and non-cancerous ovary tissue.

AsPC-1 / BxPC-3

Relative Expression (log2-ratio):-2.2446175
Number of Samples:3 / 3
Experimental AsPC-1
Human xenograft derived metastatic cancer cell line derived from mouse xenografts initiated with metastatic cells from the ascites derived from a 62 years old female Caucasian patient with pancreatic adenocarcinoma. Synonyms:AsPc-1; Aspc-1; ASPC-1; As-PC1; ASPC1; AsPC1; Aspc1; AsPc1 Cellosaurus code:
Control BxPC-3
Human pancreatic adenocarcinoma cell line derived from a 61 years old female patient. Synonyms:BxPc-3; BXPC-3; Bx-PC3; BXPC3; BxPC3; BxPc3 Cellosaurus code:

pediatric septic shock study 3 (infant; subclass A) / normal blood sample (infant)

Relative Expression (log2-ratio):-2.1609497
Number of Samples:8 / 17
Experimental pediatric septic shock study 3 (infant; subclass A)
Whole blood samples obtained from infants (1 month – 1 year) with septic shock subclass A. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. Two children did not survive. The subclass A was defined based on an empiric, discovery oriented expression filter and unsupervised hierarchical clustering. Patients in subclass A (when all age groups were pooled) had a significantly higher illness severity level (PRISM III score = 20.5, intra-quartile range (IQR) 12.5 – 32.5), a greater degree of organ failure – maximum number of organ failures 3 (IQR 3 - 4), and a higher mortality rate, a significantly higher incidence of documented Gram-positive bacterial infection and were significantly younger compared with other subclasses.
Control normal blood sample (infant)
Whole blood samples from infants (1 month – 1 year). Children who had a recent febrile illness (within 2 weeks), who recently used anti-inflammatory medications (within 2 weeks) or who had any history of chronic or acute disease associated with inflammation were excluded from the study.

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-2.1377392
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.