TOP TEN perturbations for 1552912_a_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1552912_a_at
Selected probe(set): 1561853_a_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1552912_a_at (1561853_a_at) across 6673 perturbations tested by GENEVESTIGATOR:

cytomegalovirus study 1 / C. albicans study 1

Relative Expression (log2-ratio):-2.736579
Number of Samples:3 / 3
Experimental cytomegalovirus study 1
Peripheral blood mononuclear cell samples from healthy, HIV-negative individuals were CFSE labeled and stimulated with CMV-antigens (lysate) for 6 days. CFSE-low and pathogen-specific CD4+ T cell samples were isolated by FACS sorting from stimulated PBMC´s.
Control C. albicans study 1
Peripheral blood mononuclear cell samples from healthy, HIV-negative individuals were CFSE labeled and stimulated with Candida albicans-antigens (sonicate) for 6 days. CFSE-low and pathogen-specific CD4+ T cell samples were isolated by FACS sorting from stimulated PBMC´s.

tetanus toxin study 1 / cytomegalovirus study 1

Relative Expression (log2-ratio):2.2640085
Number of Samples:3 / 3
Experimental tetanus toxin study 1
Peripheral blood mononuclear cell samples from healthy, HIV-negative individuals were CFSE labeled and stimulated with tetanus toxoid (tetanospasmin) for 6 days. CFSE-low and pathogen-specific CD4+ T cell samples were isolated by FACS sorting from stimulated PBMC´s.
Control cytomegalovirus study 1
Peripheral blood mononuclear cell samples from healthy, HIV-negative individuals were CFSE labeled and stimulated with CMV-antigens (lysate) for 6 days. CFSE-low and pathogen-specific CD4+ T cell samples were isolated by FACS sorting from stimulated PBMC´s.

NC1153 study 1 (12h; 25uM) / vehicle (DMSO) treated Kit225 cell sample (12h)

Relative Expression (log2-ratio):-1.5614595
Number of Samples:2 / 2
Experimental NC1153 study 1 (12h; 25uM)
IL-2 dependent chronic lymphocytic leukemia cell line Kit225 treated with 25 uM of Mannich base NC1153. ATC code:---
Control vehicle (DMSO) treated Kit225 cell sample (12h)
IL-2 dependent chronic lymphocytic leukemia cell line Kit225 treated with 0.1% DMSO for 12 hours.

PMA; ionomycine study 2 / unstimulated CD4 memory T-cell sample

Relative Expression (log2-ratio):1.3624363
Number of Samples:8 / 8
Experimental PMA; ionomycine study 2
CD4+ memory T cells derived from peripheral blood of healthy subjects were stimulated for 3 hours with phorbol 12-myristate 13-acetate (PMA, 10 ng/ml) and ionomycin (1 µg/ml). ATC code:---
Control unstimulated CD4 memory T-cell sample
Unstimulated CD4+ memory T cells derived from peripheral blood of healthy subjects.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):1.2014441
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

Burkitt lymphoma study 2 (immunodeficiency-associated) / Burkitt lymphoma study 1 (endemic)

Relative Expression (log2-ratio):1.0650578
Number of Samples:2 / 13
Experimental Burkitt lymphoma study 2 (immunodeficiency-associated)
Tumor tissue samples from the lymph node of children with immunodeficiency-associated Burkitt lymphoma (ID-BL).
Control Burkitt lymphoma study 1 (endemic)
Tumor tissue samples from the lymph node of children with endemic Burkitt lymphoma (eBL).

myocardial infarction study 7 (discovery) / normal vascular endothelium cell sample (discovery)

Relative Expression (log2-ratio):-0.9076252
Number of Samples:21 / 20
Experimental myocardial infarction study 7 (discovery)
Circulating CD146+ endothelial cells (CECs) isolated from patients with acute myocardial infarction (AMI) with or without ST segment deviation assigned to the discovery dataset. Arterial blood was collected in the cardiac catheterization laboratory following the placement of an arterial sheath prior to introduction of any guide wires or coronary catheters. The samples were maintained at room temperature and processed within 36 hours of collection. CECs were enriched from whole blood using CD146+ immunomagnetic separation and enumerated using the CellSearch system. AMI patients met strict diagnostic criteria including chest pain symptoms with electrocardiographic evidence of ST-segment elevation of at least 0.2 mV in two contiguous precordial leads or 0.1 mV in limb leads in addition to angiographic evidence of obstructive coronary artery disease in the setting of positive cardiac biomarkers. Inclusion criteria: 18 - 80 years of age; reliable, cooperative and willing to comply with all protocol-specified procedures if consented; able to understand and grant informed consent; acute MI defined as: clinical history and symptoms consistent with acute MI AND elevated cardiac markers (CKMB, Troponin I or T) consistent with MI AND able to complete study enrollment (consent & blood draw) within 48 hours of presentation to the study site. Exclusion criteria: significant medical condition that in the investigator's opinion may interfere with the patient's optimal participation in the study.
Control normal vascular endothelium cell sample (discovery)
Circulating CD146+ endothelial cells (CECs) isolated from healthy control patients assigned to the discovery dataset. CECs were enriched from whole blood using CD146+ immunomagnetic separation and enumerated using the CellSearch system. Inclusion criteria: 18-35 years of age; reliable, cooperative and willing to comply with all protocol-specified procedures if consented; able to understand and grant informed consent. Exclusion criteria: previous history of coronary artery disease or MI; Diabetes; Peripheral arterial disease; Hypertension (>140/90 or on blood pressure medication); sickle cell disease; acute or chronic kidney disease; acute or chronic vascular conditions, not otherwise specified; active or history of inflammation of connective tissue and vascular structures (i.e. vasculitis, rheumatoid arthritis).

ovarian tumor study 16 / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):0.8986368
Number of Samples:3 / 5
Experimental ovarian tumor study 16
Human epithelial tumor cell samples from the ovary of patients with papillary serous carcinoma. Samples were derived by laser capture microdissection (LCM).
Control normal ovarian surface epithelial cell sample
Human epithelial cell samples from histopathological normal and non-cancerous ovary tissue.

Cornelia de Lange syndrome study 1 / normal lymphoblastoid cell line sample

Relative Expression (log2-ratio):0.8608923
Number of Samples:17 / 18
Experimental Cornelia de Lange syndrome study 1
Lymphoblastoid cell line samples from severely affected Cornelia de Lange syndrome (CdLS) probands with NIPBL isoform B protein-truncating mutations.
Control normal lymphoblastoid cell line sample
Lymphoblastoid cell line samples from healthy subjects.

DLBCL study 1 (germinal center B-cell like; plasmablast-like) / DLBCL study 1 (activated B-cell like; plasmablast-like)

Relative Expression (log2-ratio):0.85718346
Number of Samples:3 / 12
Experimental DLBCL study 1 (germinal center B-cell like; plasmablast-like)
Plasmablast B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (germinal center B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS).
Control DLBCL study 1 (activated B-cell like; plasmablast-like)
Plasmablast B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (activated B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS).