TOP TEN perturbations for 1552935_at (Homo sapiens)
Organism: Homo sapiens
Gene: 1552935_at
Selected probe(set): 1552935_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 1552935_at (1552935_at) across 6673 perturbations tested by GENEVESTIGATOR:
PMA study 2 / mock treated MONO-MAC-6 cell sample
Relative Expression (log2-ratio):1.9418583Number of Samples:3 / 2
Experimental | PMA study 2 |
MONO-MAC-6 (MM6) cells were treated with 10 ng/ml phorbol 12-myristate 13-acetate (PMA). ATC code:--- | |
Control | mock treated MONO-MAC-6 cell sample |
MONO-MAC-6 (MM6) cells mock treated. |
breast cancer study 48 (PDX; metaplastic carcinoma, NOS; primary) / breast cancer study 48 (PDX; infiltrating duct carcinoma, NOS; primary)
Relative Expression (log2-ratio):1.8954906Number of Samples:8 / 60
Experimental | breast cancer study 48 (PDX; metaplastic carcinoma, NOS; primary) |
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary metaplastic carcinoma, NOS of the breast (subcutaneously implanted). | |
Control | breast cancer study 48 (PDX; infiltrating duct carcinoma, NOS; primary) |
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary infiltrating duct carcinoma, NOS of the breast (subcutaneously implanted). |
PARK2 overexpression study 1 / control transfected U251 cell sample
Relative Expression (log2-ratio):-1.8312674Number of Samples:3 / 3
Experimental | PARK2 overexpression study 1 |
Human brain glioma cell line U-251MG overexpressing ectopic wild-type PARK2 (parkin RBR E3 ubiquitin protein ligase). For generation of a stable cell line, the cells were infected with viral particles in the presence of 8 ug/mL polybrene followed by puromycin selection. | |
Control | control transfected U251 cell sample |
Human brain glioma cell line U-251MG with stable GFP expression. For generation of a cell line, the cells were infected with viral particles in the presence of 8 ug/mL polybrene followed by puromycin selection. |
PMA study 2 (shRNA cycT1) / cycT1 depletion study 2 (shRNA)
Relative Expression (log2-ratio):1.5212469Number of Samples:3 / 2
Experimental | PMA study 2 (shRNA cycT1) |
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then treated with 10ng/ml phorbol 12-myristate 13-acetate (PMA). ATC code:--- | |
Control | cycT1 depletion study 2 (shRNA) |
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then mock treated. |
PMA study 2 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample
Relative Expression (log2-ratio):1.458066Number of Samples:3 / 2
Experimental | PMA study 2 (shRNA contr.) |
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml phorbol 12-myristate 13-acetate (PMA). ATC code:--- | |
Control | mock treated / transduced MONO-MAC-6 cell sample |
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated. |
atopic dermatitis study 21 (lesional; whole skin) / normal skin tissue
Relative Expression (log2-ratio):-1.4576902Number of Samples:5 / 6
Experimental | atopic dermatitis study 21 (lesional; whole skin) |
Lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %. | |
Control | normal skin tissue |
Full thickness skin samples isolated from healthy subjects by laser capture microdissection. |
TGF-β study 11 / untreated HCC827 cell sample
Relative Expression (log2-ratio):1.3339386Number of Samples:3 / 3
Experimental | TGF-β study 11 |
HCC827 cells were cultured for 3-5 weeks in the presence of 2ng/ml of TGF-β, to induce epithelial-mesenchymal transition (EMT). 24 hours prior analysis cells were re-seeded without TGF-β. | |
Control | untreated HCC827 cell sample |
HCC827 cells grown in standard media. |
HIF-1a/HIF-2a depletion study 1 (normoxia; AB81) / control AB81 cell sample (normoxia)
Relative Expression (log2-ratio):-1.245431Number of Samples:3 / 3
Experimental | HIF-1a/HIF-2a depletion study 1 (normoxia; AB81) |
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with normoxia (20% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. | |
Control | control AB81 cell sample (normoxia) |
Human podocyte cell line AB81 treated with normoxia (20% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. |
HIF-2a depletion study 1 (normoxia; AB81) / control AB81 cell sample (normoxia)
Relative Expression (log2-ratio):-1.1952105Number of Samples:3 / 3
Experimental | HIF-2a depletion study 1 (normoxia; AB81) |
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-2a (Hypoxia-inducible factor 2-alpha) 24 hours after treatment with normoxia (20% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. | |
Control | control AB81 cell sample (normoxia) |
Human podocyte cell line AB81 treated with normoxia (20% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. |
adrenal gland cancer study 4 / normal adrenal cortex tissue
Relative Expression (log2-ratio):-1.1657438Number of Samples:43 / 4
Experimental | adrenal gland cancer study 4 |
Primary tumor tissue samples of the adrenal cortex from patients with adrenocortical carcinoma (ACC). | |
Control | normal adrenal cortex tissue |
Histological normal adrenal gland cortical tissue samples derived after autopsy. |