TOP TEN perturbations for 1552972_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1552972_at
Selected probe(set): 1552972_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1552972_at (1552972_at) across 6593 perturbations tested by GENEVESTIGATOR:

TGF-β study 11 / untreated HCC827 cell sample

Relative Expression (log2-ratio):3.2404075
Number of Samples:3 / 3
Experimental TGF-β study 11
HCC827 cells were cultured for 3-5 weeks in the presence of 2ng/ml of TGF-β, to induce epithelial-mesenchymal transition (EMT). 24 hours prior analysis cells were re-seeded without TGF-β.
Control untreated HCC827 cell sample
HCC827 cells grown in standard media.

HIF-1a/HIF-2a depletion study 1 (normoxia; HK2) / control HK2 cell sample (normoxia)

Relative Expression (log2-ratio):-2.672286
Number of Samples:3 / 6
Experimental HIF-1a/HIF-2a depletion study 1 (normoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with normoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control control HK2 cell sample (normoxia)
Proximal tubule epithelial cell line HK-2 treated with normoxia (20% O2) for 24 hours. HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) / hypoxia study 11 (HK2)

Relative Expression (log2-ratio):-2.5978112
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control hypoxia study 11 (HK2)
Proximal tubule epithelial cell line HK-2 treated with hypoxia (1% O2) for 24 hours. HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):-2.5343642
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

HIF-2a depletion study 1 (hypoxia; HK2) / hypoxia study 11 (HK2)

Relative Expression (log2-ratio):-2.156868
Number of Samples:3 / 3
Experimental HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-2a (Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control hypoxia study 11 (HK2)
Proximal tubule epithelial cell line HK-2 treated with hypoxia (1% O2) for 24 hours. HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

TGF-β study 12 / untreated NCI-H358 cell sample

Relative Expression (log2-ratio):2.1199894
Number of Samples:3 / 3
Experimental TGF-β study 12
NCI-H358 cells were cultured for 2-3 weeks in the presence of 2ng/ml of TGF-β, to induce epithelial-mesenchymal transition (EMT). 24 hours prior analysis cells were re-seeded without TGF-β.
Control untreated NCI-H358 cell sample
NCI-H358 cells grown in standard media.

atopic dermatitis study 12 (lesional; adults) / atopic dermatitis study 12 (lesional; children)

Relative Expression (log2-ratio):-2.0646105
Number of Samples:20 / 18
Experimental atopic dermatitis study 12 (lesional; adults)
Lesional skin biopsy samples from adult patients (age range 18-73 years) with long-standing atopic dermatitis.
Control atopic dermatitis study 12 (lesional; children)
Lesional popliteal skin biopsy samples from pediatric patients (age range 3 months-5 years) with early-onset atopic dermatitis. All biopsy specimens were from chronic lesions present for more than 72 hours. All patients had moderate-to-severe disease with recent-onset (within the previous 6 months). Systemic immunosuppressants within the past 4 weeks, topical steroids or immunomodulators within 1 week, or moisturizers within 12 hours before evaluation were restricted. Patients with active skin infections were excluded.

HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) / HIF-1a depletion study 2 (hypoxia; HK2)

Relative Expression (log2-ratio):-1.8259139
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control HIF-1a depletion study 2 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

atopic dermatitis study 12 (lesional; children) / normal skin tissue (children)

Relative Expression (log2-ratio):1.8224163
Number of Samples:18 / 15
Experimental atopic dermatitis study 12 (lesional; children)
Lesional popliteal skin biopsy samples from pediatric patients (age range 3 months-5 years) with early-onset atopic dermatitis. All biopsy specimens were from chronic lesions present for more than 72 hours. All patients had moderate-to-severe disease with recent-onset (within the previous 6 months). Systemic immunosuppressants within the past 4 weeks, topical steroids or immunomodulators within 1 week, or moisturizers within 12 hours before evaluation were restricted. Patients with active skin infections were excluded.
Control normal skin tissue (children)
Skin biopsy samples from healthy children (age range 4 months – 3 years) without personal/familial atopy.

mitomycin study 3 (HPV8 E6; 18h; 2ug/ml) / vehicle (water) treated N/Tert-8E6 cell sample

Relative Expression (log2-ratio):1.7679877
Number of Samples:3 / 3
Experimental mitomycin study 3 (HPV8 E6; 18h; 2ug/ml)
N/Tert-8E6 human immortalized keratinocytes treated with mitomycin C (2ug/ml) for 18h. Mitomycin C was used for the induction of DNA damage. ATC code:
Control vehicle (water) treated N/Tert-8E6 cell sample
Human immortalized keratinocyte cell line N/Tert-8E6 treated with water for 18 hours. The cells were grown to approximately 30% confluence in keratinocyte serum-free medium (K-SFM), following which they were harvested by trypsinization and freezing at -80ºC.