TOP TEN perturbations for 1553024_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553024_at
Selected probe(set): 1553024_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553024_at (1553024_at) across 6672 perturbations tested by GENEVESTIGATOR:

systemic lupus erythematosus study 13 (untreated) / normal PBMC sample

Relative Expression (log2-ratio):-1.4519649
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (untreated)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists.
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

epigallocatechin gallate study 1 (45840ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):1.0938907
Number of Samples:3 / 17
Experimental epigallocatechin gallate study 1 (45840ng/ml)
Bronchial epithelial cells (NHBE) treated with epigallocatechin gallate (45840ng/ml; vendor: Santa Cruz / catalog number: sc-200802 / catalog name: (-)-Epigallocatechin Gallate) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:---
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

systemic lupus erythematosus study 3 / normal blood sample

Relative Expression (log2-ratio):1.0570221
Number of Samples:89 / 30
Experimental systemic lupus erythematosus study 3
Blood samples derived from patients with anti-ribonuclear protein-positive (anti-RNP+) systemic lupus erythematosus (SLE).
Control normal blood sample
Blood samples from healthy individuals.

exercise study 33 (OT; slow twitch; pre-exerc.) / exercise study 33 (OUT; slow twitch; pre-exerc.)

Relative Expression (log2-ratio):1.0129571
Number of Samples:4 / 4
Experimental exercise study 33 (OT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from old trained (OT) women (85±1 years) before the 36th (last) exercise session. Participants underwent 12 weeks of progressive resistance training including 36 training sessions (3 days/week) with three sets of 10 bilateral knee extensions at 70–75% of their one-repetition maximum (1 RM). Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.
Control exercise study 33 (OUT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from old untrained (OUT) women (85±1 years) before the first exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.

Dengue hemorrhagic fever study 1 (3d) / Dengue hemorrhagic fever study 1 (convalescent)

Relative Expression (log2-ratio):0.85510635
Number of Samples:8 / 12
Experimental Dengue hemorrhagic fever study 1 (3d)
Peripheral blood mononuclear cell (PBMC) samples derived from the blood of Venezuelan individuals 3 days after the onset of Dengue hemorrhagic fever.
Control Dengue hemorrhagic fever study 1 (convalescent)
Peripheral blood mononuclear cell (PBMC) samples derived from the blood of Venezuelan individuals 28 days after the onset of Dengue hemorrhagic fever.

Daoy-BMI1 KD-PCGF2 KD / Daoy-ALK KD

Relative Expression (log2-ratio):-0.8130951
Number of Samples:3 / 3
Experimental Daoy-BMI1 KD-PCGF2 KD
Daoy cells with knockdown of BMI1 and polycomb group ring finger 2 (PCGF2 or mel-18). Daoy cells are human primary cancer cell line derived from the cerebellum (posterior fossa) of a patient with desmoplastic medulloblastoma. Parental cell line:: Daoy
Control Daoy-ALK KD
Daoy cells with knockdown of anaplastic lymphoma kinase (ALK), which is only expressed in lymphoid cells. Daoy cells are human primary cancer cell line derived from the cerebellum (posterior fossa) of a patient with desmoplastic medulloblastoma. Parental cell line:: Daoy

Daoy-BMI1 KD / Daoy-ALK KD

Relative Expression (log2-ratio):-0.7231345
Number of Samples:3 / 3
Experimental Daoy-BMI1 KD
Daoy cells with knockdown of B lymphoma Mo-MLV insertion region 1 (BMI1). Daoy cells are human primary cancer cell line derived from the cerebellum (posterior fossa) of a patient with desmoplastic medulloblastoma. Parental cell line:: Daoy
Control Daoy-ALK KD
Daoy cells with knockdown of anaplastic lymphoma kinase (ALK), which is only expressed in lymphoid cells. Daoy cells are human primary cancer cell line derived from the cerebellum (posterior fossa) of a patient with desmoplastic medulloblastoma. Parental cell line:: Daoy

Daoy-PCGF2 KD / Daoy-ALK KD

Relative Expression (log2-ratio):-0.7094817
Number of Samples:3 / 3
Experimental Daoy-PCGF2 KD
Daoy cells with knockdown of a polycomb group ring finger 2 (PCGF2 or mel-18). Daoy cells are human primary cancer cell line derived from the cerebellum (posterior fossa) of a patient with desmoplastic medulloblastoma. Parental cell line:: Daoy
Control Daoy-ALK KD
Daoy cells with knockdown of anaplastic lymphoma kinase (ALK), which is only expressed in lymphoid cells. Daoy cells are human primary cancer cell line derived from the cerebellum (posterior fossa) of a patient with desmoplastic medulloblastoma. Parental cell line:: Daoy

FSL-1 study 1 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-0.6987443
Number of Samples:3 / 17
Experimental FSL-1 study 1
Bronchial epithelial cells (NHBE) treated with FSL-1 (1000 ng/ml; vendor: InvivoGen / catalog number: tlrl-fsl / catalog name: FSL-1) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

HB-EGF study 1 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-0.66887426
Number of Samples:3 / 17
Experimental HB-EGF study 1
Bronchial epithelial cells (NHBE) treated with 50 ng/ml recombinant human proheparin-binding EGF-like growth factor (HB-EGF; vendor: PeproTech / catalog number: 100-47 / catalog name: HB-EGF) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).