TOP TEN perturbations for 1553226_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553226_at
Selected probe(set): 1553226_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553226_at (1553226_at) across 6672 perturbations tested by GENEVESTIGATOR:

RAF1 overexpr. study 1 (retrovirus) / control virus infected MCF7 cell sample

Relative Expression (log2-ratio):-5.181572
Number of Samples:2 / 2
Experimental RAF1 overexpr. study 1 (retrovirus)
MCF-7 cell line stably transfected with retrovirus containing cDNA for RAF1. The following retroviral construct was used: pLNCRaf-1 contains human Raf-1 cDNA encoding an amino terminally truncated form of Raf-1 that is constitutively active, which is inserted into retroviral vector pLNCX encoding neomycin resistance gene (NeoR). MCF7 cells were maintained in EMEM supplemented with 10% FBS and 20ug/ml insulin.
Control control virus infected MCF7 cell sample
MCF7 cell line transfected with pZipneo retrovirus, which is an empty retroviral vector encoding neomycine resistance gene (NeoR). MCF7 cells were maintained in EMEM supplemented with 10% FBS and 20ug/ml insulin.

nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector) / flagellin study 4 (control vector; 500 ng/ml)

Relative Expression (log2-ratio):-2.6099224
Number of Samples:6 / 2
Experimental nutlin-3 (10uM); flagellin (500 ng/ml) study 1 (control vector)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 45 hours followed by an additional 3 hours treatment of nutlin-3 (10uM) and flagellin (500 ng/ml). Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---
Control flagellin study 4 (control vector; 500 ng/ml)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with DMSO 0.1% for 45 hours followed by an additional 3 hours with flagellin (500 ng/ml). Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin.

echinomycin study 1 / deferoxamine study 5

Relative Expression (log2-ratio):2.575859
Number of Samples:3 / 3
Experimental echinomycin study 1
Echinomycin (100nM; 2h) treated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:---
Control deferoxamine study 5
Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:

estradiol study 18 (late) / vehicle treated MCF-7 cell sample

Relative Expression (log2-ratio):2.5676336
Number of Samples:3 / 3
Experimental estradiol study 18 (late)
MCF7 cells were grown to 30-50% confluency and exposed to 10 nM 17beta-estradiol (E2) for 48 hours. ATC code:
Control vehicle treated MCF-7 cell sample
MCF7 cells were grown to 30-50% confluency and exposed to vehicle only for 48 hours.

nutlin-3 study 2 (control vector; 10uM) / vehicle treated MCF-7-con cell sample

Relative Expression (log2-ratio):-2.206771
Number of Samples:6 / 3
Experimental nutlin-3 study 2 (control vector; 10uM)
Human breast adenocarcinoma cells MCF-7 expressing wild type p53, stably transfected with control empty vector pSUPER, treated with nutlin-3 (10 uM) for 48 hours. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin. ATC code:---
Control vehicle treated MCF-7-con cell sample
Human breast adenocarcinoma cells MCF-7 expressing wild type p53 stably transfected with control empty vector pSUPER, vehicle treated with DMSO (0.1%) for 48 hours. Cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated FBS and 50 U/ml of penicillin, 50 ug/ml of streptomycin.

BEZ235 study 1 (3.606 uM) / vehicle (DMSO) treated A2058 cell samples

Relative Expression (log2-ratio):-1.8285046
Number of Samples:4 / 4
Experimental BEZ235 study 1 (3.606 uM)
A2058 cells treated with 3.606 uM NVP-BEZ235 for 24hours. The concentration corresponding to the IC90 of BKM120. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine. BEZ235 is a mTOR/PI3K inhibitor; alternative names: [NVP-BEZ235] ATC code:---
Control vehicle (DMSO) treated A2058 cell samples
A2058 cells treated with vehicle (0.1% DMSO) for 24hours. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine.

deferoxamine study 5 / untreated U251 astroglioma cell sample

Relative Expression (log2-ratio):-1.563262
Number of Samples:3 / 2
Experimental deferoxamine study 5
Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:
Control untreated U251 astroglioma cell sample
Untreated human astroglioma (U251) cells not stimulated with deferoxamine (DFO).

estradiol study 18 (intermediate) / vehicle treated MCF-7 cell sample

Relative Expression (log2-ratio):1.5610437
Number of Samples:3 / 3
Experimental estradiol study 18 (intermediate)
MCF7 cells were grown to 30-50% confluency and exposed to 10 nM 17beta-estradiol (E2) for 24 hours. ATC code:
Control vehicle treated MCF-7 cell sample
MCF7 cells were grown to 30-50% confluency and exposed to vehicle only for 24 hours.

MCF-7-MT2A-MYC / MCF-7-con

Relative Expression (log2-ratio):1.4937983
Number of Samples:3 / 3
Experimental MCF-7-MT2A-MYC
MCF-7 cells with metal inducible expression of human c-Myc. MCF-7 cells contain a 252bp fragment of metallothionein promoter from MT2A gene which allows inducible activation of c-MYC expression by metal ions (ZN2+). MCF-7 is human metastatic cancer cell line derived from the pleural effusion of a patient with adenocarcinoma of the breast. Parental cell line:: MCF-7 Synonyms:pΔMT-c-MYC
Control MCF-7-con
MCF-7 cell line modified by stable control transfection. Parental cell line:: MCF-7

ARC study 1 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):-1.4933119
Number of Samples:2 / 2
Experimental ARC study 1
MCF7 cells treated with 2uM ARC (4-amino-6-hydrazino-7-beta-d-ribofuranosyl-7H-pyrrolo-(2, 3-d)-pyrimidine-5-carboxamide, NSC 188491) for 24 hours. ATC code:---
Control untreated MCF-7 cell sample
MCF-7 cells untreated.