TOP TEN perturbations for 1553241_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553241_at
Selected probe(set): 1553241_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553241_at (1553241_at) across 6672 perturbations tested by GENEVESTIGATOR:

BMP-2 study 4 / vehicle treated pulmonary artery endothelial cell sample

Relative Expression (log2-ratio):1.2381973
Number of Samples:4 / 4
Experimental BMP-2 study 4
Pulmonary artery endothelial cells (PAEC) treated for 4 hours with 10 ng/ml BMP2, bone morphogenetic protein 2 (in new media). Before treatment cells were kept for 12 hours in M199 media with 0.1% FBS. PAEC were cultured in EGM-2 media (2% FBS).
Control vehicle treated pulmonary artery endothelial cell sample
Pulmonary artery endothelial cell (PAEC) treated for 4 hours with vehicle (in new media). Before treatment cells were kept for 12 hours in M199 media with 0.1% FBS. PAEC were cultured in EGM-2 media (2% FBS).

exercise study 33 (OT; slow twitch; pre-exerc.) / exercise study 33 (OUT; slow twitch; pre-exerc.)

Relative Expression (log2-ratio):0.8150816
Number of Samples:4 / 4
Experimental exercise study 33 (OT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from old trained (OT) women (85±1 years) before the 36th (last) exercise session. Participants underwent 12 weeks of progressive resistance training including 36 training sessions (3 days/week) with three sets of 10 bilateral knee extensions at 70–75% of their one-repetition maximum (1 RM). Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.
Control exercise study 33 (OUT; slow twitch; pre-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from old untrained (OUT) women (85±1 years) before the first exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.

systemic lupus erythematosus study 3 / normal blood sample

Relative Expression (log2-ratio):0.7895298
Number of Samples:89 / 30
Experimental systemic lupus erythematosus study 3
Blood samples derived from patients with anti-ribonuclear protein-positive (anti-RNP+) systemic lupus erythematosus (SLE).
Control normal blood sample
Blood samples from healthy individuals.

sodium chloride study 1 (8766ug/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):0.7451973
Number of Samples:2 / 17
Experimental sodium chloride study 1 (8766ug/ml)
Bronchial epithelial cells (NHBE) treated with NaCl (8766ug/ml; vendor: Sigma / catalog number: S5886 / catalog name: Sodium Chloride [7647-14-5]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:---
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

exercise study 33 (OUT; slow twitch; post-exerc.) / exercise study 33 (YUT; slow twitch; post-exerc.)

Relative Expression (log2-ratio):-0.7381048
Number of Samples:3 / 5
Experimental exercise study 33 (OUT; slow twitch; post-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from old untrained (OUT) women (85±1 years) 4 hours after the first resistance exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.
Control exercise study 33 (YUT; slow twitch; post-exerc.)
Slow twitch myosin heavy chain muscle fibers (MHC) I were obtained from vastus lateralis muscle. Biopsies were taken from young untrained (YUT) women (23±2 years old) 4 hours after the first resistance exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.

myotonic dystrophy study 4 (deltoid muscle; DM2) / normal skeletal muscle tisuue (fetal)

Relative Expression (log2-ratio):0.7247968
Number of Samples:3 / 3
Experimental myotonic dystrophy study 4 (deltoid muscle; DM2)
Deltoid muscle biopsies obtained from patients with myotonic dystrophy type 2 (DM2).
Control normal skeletal muscle tisuue (fetal)
Commercially purchased normal fetal skeletal muscle tissue samples.

myotonic dystrophy study 4 (biceps brachii; DM2) / normal skeletal muscle tisuue (fetal)

Relative Expression (log2-ratio):0.72421026
Number of Samples:3 / 3
Experimental myotonic dystrophy study 4 (biceps brachii; DM2)
Biceps brachii biopsies obtained from patients with myotonic dystrophy type 2 (DM2).
Control normal skeletal muscle tisuue (fetal)
Commercially purchased normal fetal skeletal muscle tissue samples.

BMP-6 study 2 / vehicle treated pulmonary artery endothelial cell sample

Relative Expression (log2-ratio):0.7218933
Number of Samples:4 / 4
Experimental BMP-6 study 2
Pulmonary artery endothelial cells (PAEC) treated for 4 hours with 10 ng/ml BMP6, bone morphogenetic protein 6 (in new media). Before treatment cells were kept for 12 hours in M199 media with 0.1% FBS. PAEC cells were cultured in EGM-2 media (2% FBS).
Control vehicle treated pulmonary artery endothelial cell sample
Pulmonary artery endothelial cell (PAEC) treated for 4 hours with vehicle (in new media). Before treatment cells were kept for 12 hours in M199 media with 0.1% FBS. PAEC were cultured in EGM-2 media (2% FBS).

systemic lupus erythematosus study 13 (untreated) / normal PBMC sample

Relative Expression (log2-ratio):-0.71897507
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (untreated)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists.
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

myotonic dystrophy study 4 (biceps brachii; DM1) / normal skeletal muscle tisuue (fetal)

Relative Expression (log2-ratio):0.7179694
Number of Samples:3 / 3
Experimental myotonic dystrophy study 4 (biceps brachii; DM1)
Biceps brachii biopsies obtained from patients with myotonic dystrophy type 1 (DM1).
Control normal skeletal muscle tisuue (fetal)
Commercially purchased normal fetal skeletal muscle tissue samples.