TOP TEN perturbations for 1553286_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553286_at
Selected probe(set): 1553286_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553286_at (1553286_at) across 6672 perturbations tested by GENEVESTIGATOR:

wound healing study 2 (ex vivo; DMSO) / normal skin tissue (ex vivo)

Relative Expression (log2-ratio):4.410942
Number of Samples:3 / 3
Experimental wound healing study 2 (ex vivo; DMSO)
Ex vivo skin samples obtained from healthy donors following reduction surgery of abdomen, and incubated in culture medium containing DMSO for 4 days. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection. Skin was sliced into 1x10 mm slices and incubated in keratinocyte medium for four days with 1:1000 fold dilution of DMSO. The cultivation was performed in serum-free keratinocyte medium supplemented with transferrin, hEGF (0.15 ng/mL), 0.5 mg/mL hydrocortisone, gentamicin, amphotericin B, and epinephrine but without insulin.
Control normal skin tissue (ex vivo)
Normal skin samples obtained from healthy donors following reduction surgery of abdomen. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection.

wound healing study 2 (ex vivo; AG1478) / normal skin tissue (ex vivo)

Relative Expression (log2-ratio):3.4480114
Number of Samples:3 / 3
Experimental wound healing study 2 (ex vivo; AG1478)
Ex vivo skin samples obtained from healthy donors following reduction surgery of abdomen, and incubated in culture medium containing AG1478 for 4 days. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection. Skin was sliced into 1x10 mm slices and incubated in keratinocyte medium for four days with 10 micromolar AG1478 (dissolved in DMSO). The cultivation was performed in serum-free keratinocyte medium supplemented with transferrin, hEGF (0.15 ng/mL), 0.5 mg/mL hydrocortisone, gentamicin, amphotericin B, and epinephrine but without insulin. AG1478 is EGFR kinase inhibitor. ATC code:---
Control normal skin tissue (ex vivo)
Normal skin samples obtained from healthy donors following reduction surgery of abdomen. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection.

peptidoglycan study 1 / mock treated neonatal neutrophils

Relative Expression (log2-ratio):2.9069214
Number of Samples:3 / 3
Experimental peptidoglycan study 1
Healthy neonatal neutrophils with ≥ 99% purity isolated from cord blood samples collected after normal, full-term, vaginal delivery treated with peptidoglycan isolated from S. aureus (10 ug/ml) for 4 hours.
Control mock treated neonatal neutrophils
Healthy neonatal neutrophils with ≥ 99% purity isolated from cord blood samples collected after normal, full-term, vaginal delivery mock treated for 4 hours.

Barrett's esophagus study 2 (doca) early / untreated CP-A hTERT cell sample

Relative Expression (log2-ratio):2.878395
Number of Samples:2 / 2
Experimental Barrett's esophagus study 2 (doca) early
CP-A hTERT Barrett's esophagus cells 2 hours after 15 minute exposure to deoxycholic acid, pH 4.5.
Control untreated CP-A hTERT cell sample
CP-A hTERT Barrett's esophagus cells unexposed.

tetracycline study 7 (2000uM) / vehicle (DMSO) treated HepG2 sample

Relative Expression (log2-ratio):2.469678
Number of Samples:3 / 21
Experimental tetracycline study 7 (2000uM)
HepG2 cells treated with compound: tetracycline (2000uM; CAS no.:60-54-8) for 24 hours. Tetracycline is hepatotoxic and may cause steatosis. HepG2 cells were treated with the IC20 concentration measured after 24 hours. ATC code:, , , , ,
Control vehicle (DMSO) treated HepG2 sample
HepG2 cells treated with DMSO (0.5% v/v) as solvent control for 24 hours.

spina bifida study 1 / normal amniocyte sample

Relative Expression (log2-ratio):-2.3925524
Number of Samples:4 / 5
Experimental spina bifida study 1
Fetal mRNA isolated from amniocytes obtained from pregnant women carrying fetuses with spina bifida.
Control normal amniocyte sample
Fetal mRNA isolated from amniocytes obtained from pregnant women who underwent routine genetic amniocentesis.

smoking study 11 (late) / filtered air treated bronchial epithelial cell sample (non-smoker)

Relative Expression (log2-ratio):2.3419685
Number of Samples:9 / 9
Experimental smoking study 11 (late)
Bronchial epithelial cells from non-smoker donors exposed to 1:50 dose (1.84ug/cm2) of diluted mainstream cigarette smoke for 1 hour following a 23 hours recovery period.
Control filtered air treated bronchial epithelial cell sample (non-smoker)
Bronchial epithelial cells from non-smoker donors exposed to filtered for 1 hour following a 23 hours recovery period.

formaldehyde study 2 (4500ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-2.309185
Number of Samples:3 / 3
Experimental formaldehyde study 2 (4500ug/ml)
Bronchial epithelial cells (NHBE) treated with 4500 ug/ml formaldehyde (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 8 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control vehicle (EtOH) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 8 hours. NHBE cells were derived from a 60 year old male non-smoker.

prostate cancer study 8 (p. canc) / prostate cancer study 8 (psfmc)

Relative Expression (log2-ratio):2.2101765
Number of Samples:3 / 5
Experimental prostate cancer study 8 (p. canc)
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (psfmc)
CD49a+ FACS sorted prostate stromal fibromuscular cell (psfmc) samples from patients with primary prostate cancer collected after radical prostatectomy.

smoking study 53 (28 min) / normal sham-exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):2.1392422
Number of Samples:3 / 3
Experimental smoking study 53 (28 min)
Normal human bronchial/tracheal epithelial (NHBE) cells were treated for 28 minutes with diluted cigarette smoke (15% v/v) followed by 24 hours recovery phase with fresh culture media. NHBE cells were cultured as organotypic cultures allowing an air-liquid interface. Seven puffs per cigarette (3R4F reference Kentucky cigarettes) and one puff per minute of exposure were used, resulting in 7.74 µg of Total Particulate Matter and 8.3 µg of CO / well /minute inside the exposure chamber. Cigarette smoke was generated on the VC 10 smoking robot in compliance with the International Organization for Standardization smoking regimen. Number of cigarettes varied to adjust to the exposure times.
Control normal sham-exposed bronchial epithelial cell sample
Normal human bronchial/tracheal epithelial (NHBE) cells were sham-exposed for 28 minutes to 100 % synthetic air (v/v; 15% oxygen and 85% nitrogen) followed by 24 hours recovery phase with fresh culture media. NHBE cells were cultured as organotypic cultures allowing an air-liquid interface.