TOP TEN perturbations for 1553298_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553298_at
Selected probe(set): 1553298_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553298_at (1553298_at) across 6672 perturbations tested by GENEVESTIGATOR:

HIF-1a depletion study 2 (hypoxia; HK2) / HIF-1a depletion study 2 (normoxia; HK2)

Relative Expression (log2-ratio):1.0507236
Number of Samples:3 / 3
Experimental HIF-1a depletion study 2 (hypoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with hypoxia (1% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control HIF-1a depletion study 2 (normoxia; HK2)
Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with normoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

Sjogren's syndrome study 2 (pSS) / Sjogren's syndrome study 2 (non-pSS)

Relative Expression (log2-ratio):-1.01861
Number of Samples:7 / 14
Experimental Sjogren's syndrome study 2 (pSS)
Incisional biopsy of one parotid gland of patients who fulfilled the 2002 AECG criteria for primary Sjogren's syndrome (pSS). Patients were at least 21 years old.
Control Sjogren's syndrome study 2 (non-pSS)
Incisional biopsy of one parotid gland of patients with Sicca-syndrome who did not fulfill the 2002 AECG criteria for primary Sjogren's syndrome (non-pSS Sicca). Patients were at least 21 years old.

ELK1 depletion study 1 (siRNA) / control siRNA transfected MCF10A cell sample

Relative Expression (log2-ratio):0.8088341
Number of Samples:3 / 3
Experimental ELK1 depletion study 1 (siRNA)
MCF10A cell line transfected with 20 nM targeting ELK1 for 48 hours. MCF10A cells were plated out into a mixture of 83% growth medium (DMEM/F12 containing 5% horse serum, 10 mg/ml insulin, 100 ng/ml cholera toxin and 0.5 mg/ml hydrocortisone), 17% OptiMEM (Invitrogen), 20 nM siRNA and 0.33% Lipofectamine2000 followed by replacement of the transfection mix with appropriate growth media after 12 hours.
Control control siRNA transfected MCF10A cell sample
MCF10A cell line transfected with 20 nM control siRNA (siGAPDH) for 48 hours. MCF10A cells were plated out into a mixture of 83% growth medium (DMEM/F12 containing 5% horse serum, 10 mg/ml insulin, 100 ng/ml cholera toxin and 0.5 mg/ml hydrocortisone), 17% OptiMEM (Invitrogen), 20 nM siRNA and 0.33% Lipofectamine2000 followed by replacement of the transfection mix with appropriate growth media after 12 hours.

ovarian tumor study 16 / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):0.79079866
Number of Samples:3 / 5
Experimental ovarian tumor study 16
Human epithelial tumor cell samples from the ovary of patients with papillary serous carcinoma. Samples were derived by laser capture microdissection (LCM).
Control normal ovarian surface epithelial cell sample
Human epithelial cell samples from histopathological normal and non-cancerous ovary tissue.

LPS study 4 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample

Relative Expression (log2-ratio):0.7897959
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA contr.)
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated / transduced MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.

hypoxia study 11 (HK2) / control HK2 cell sample (normoxia)

Relative Expression (log2-ratio):0.73829126
Number of Samples:3 / 6
Experimental hypoxia study 11 (HK2)
Proximal tubule epithelial cell line HK-2 treated with hypoxia (1% O2) for 24 hours. HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.
Control control HK2 cell sample (normoxia)
Proximal tubule epithelial cell line HK-2 treated with normoxia (20% O2) for 24 hours. HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C.

colorectal adenoma study 4 (large) / adjacent colon mucosa tissue

Relative Expression (log2-ratio):0.7291951
Number of Samples:3 / 7
Experimental colorectal adenoma study 4 (large)
Rectum biopsies of colorectal adenomas (size: 3cm - 4cm).
Control adjacent colon mucosa tissue
Biopsies of rectum mucosa from adjacent tumor tissue.

unknown infection study 1 (IBS) / normal rectal tissue

Relative Expression (log2-ratio):-0.6685591
Number of Samples:15 / 37
Experimental unknown infection study 1 (IBS)
Rectal tissue biopsies from patients with irritable bowel disease after an unknown bacterial infection. IBS was diagnosed according to the Rome II criteria. Patients suffering from lactose intolerance, celiac diseases and bleeding or clotting disorders were excluded. Pregnant women, patients having gastrointestinal surgery, persons having an alcohol or drug dependence history and patients taking medications which could alter gastrointestinal motility or permeability (such as aspirin or non-steroidal anti-inflammatory drugs) were also excluded from the study.
Control normal rectal tissue
Rectal tissue biopsies derived from healthy volunteers. All individuals had no gastrointestinal problem at the time of experiment and within the previous 6 months.

Langerhans cell histiocytosis study 2 (multisystem) / normal epidermal Langerhans cell sample

Relative Expression (log2-ratio):0.65629244
Number of Samples:2 / 10
Experimental Langerhans cell histiocytosis study 2 (multisystem)
Langerhans cell histiocytes (LCH, CD207+) samples isolated from LCH lesions of patients with multifocal, multisystem (disseminated) disease. Both patients received chemotherapy. Sites of LCH lesions: subj.ID:1 (skin, lungs, multiple skull, mastoid, gingiva), subj.ID:13 (mandible, skull, vertebrae, recurrent orbit, liver, spleen, pituitary gland).
Control normal epidermal Langerhans cell sample
Normal epidermal Langerhans cells (CD207+) were isolated from skin of children donors (age < 18 years). The Langerhans cells were isolated from presumably healthy tissue.

systemic lupus erythematosus study 13 (untreated) / normal PBMC sample

Relative Expression (log2-ratio):-0.634593
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (untreated)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists.
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.