TOP TEN perturbations for 1553311_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553311_at
Selected probe(set): 1553311_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553311_at (1553311_at) across 6672 perturbations tested by GENEVESTIGATOR:

AsPC-1 / BxPC-3

Relative Expression (log2-ratio):-4.4049945
Number of Samples:3 / 3
Experimental AsPC-1
Human xenograft derived metastatic cancer cell line derived from mouse xenografts initiated with metastatic cells from the ascites derived from a 62 years old female Caucasian patient with pancreatic adenocarcinoma. Synonyms:AsPc-1; Aspc-1; ASPC-1; As-PC1; ASPC1; AsPC1; Aspc1; AsPc1 Cellosaurus code:
Control BxPC-3
Human pancreatic adenocarcinoma cell line derived from a 61 years old female patient. Synonyms:BxPc-3; BXPC-3; Bx-PC3; BXPC3; BxPC3; BxPc3 Cellosaurus code:

calcitriol study 7 (10nM; 18h) / vitamin A study 3 (10nM; 18h)

Relative Expression (log2-ratio):4.052593
Number of Samples:4 / 4
Experimental calcitriol study 7 (10nM; 18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with 10 nM calcitriol (1alpha, 25-Dihydroxyvitamin D3; 1,25(OH)2D3) for 18 h. ATC code:,
Control vitamin A study 3 (10nM; 18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with 10 nM ATRA (all-trans retinoic acid, biologically active form of vitamin A) for 18 h. ATC code:---

calcitriol study 7 (10nM; 18h) / vehicle treated peripheral blood monocyte sample (18h)

Relative Expression (log2-ratio):3.829268
Number of Samples:4 / 4
Experimental calcitriol study 7 (10nM; 18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with 10 nM calcitriol (1alpha, 25-Dihydroxyvitamin D3; 1,25(OH)2D3) for 18 h. ATC code:,
Control vehicle treated peripheral blood monocyte sample (18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with vehicle for 18 h.

FGFR3 (S249C) overexpression study 1 / control vector transfected TERT-NHUC cell sample

Relative Expression (log2-ratio):3.2793946
Number of Samples:3 / 3
Experimental FGFR3 (S249C) overexpression study 1
Normal human urothelial cell line TERT-NHUC stably transduced to express Fibroblast Growth Factor Receptor 3 (FGFR3) mutation S249C.
Control control vector transfected TERT-NHUC cell sample
Normal human urothelial cell line TERT-NHUC stably transduced to express an empty vector (pFB).

T-cell isolation study 11 / T-cell isolation study 6

Relative Expression (log2-ratio):-3.0583591
Number of Samples:2 / 2
Experimental T-cell isolation study 11
CD4+ resting memory T-cell were isolated from peripheral blood buffy coat of healthy donors after storage for 24 hours at 20°C. The cell fraction was first enriched via density gradient centrifugation with LSM 1077 Ficoll and than FACS sorted as CD3+/CD4+/CD45RO+/CD45RA- population.
Control T-cell isolation study 6
CD4+ resting memory T-cell were isolated from whole peripheral blood of healthy donors with no delay. The cell fraction was first enriched via density gradient centrifugation with LSM 1077 Ficoll and than FACS sorted as CD3+/CD4+/CD45RO+/CD45RA- population.

CD44s overexpr. study 1 / normal HEK-293 cell sample

Relative Expression (log2-ratio):2.8758898
Number of Samples:3 / 3
Experimental CD44s overexpr. study 1
Human embryonic kidney cell line HEK-293 transfected with pcDNA3.1(-) vector containing codon-optimized human CD44 sequence for expression.
Control normal HEK-293 cell sample
Untransfected, native human embryonic kidney cell line HEK-293 cell samples.

PMA study 6 (500ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):2.862894
Number of Samples:3 / 17
Experimental PMA study 6 (500ng/ml)
Bronchial epithelial cells (NHBE) treated with tetradecanoylphorbol acetate (500ng/ml; vendor: Sigma / catalog number: P1585 / catalog name: Phorbol 12-myristate 13-acetate [16561-29-8]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:---
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):-2.8244867
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

rheumatoid arthritis study 31 / TNF-ɑ study 20

Relative Expression (log2-ratio):2.7072635
Number of Samples:4 / 3
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocytes from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control TNF-ɑ study 20
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml TNFα in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

formaldehyde study 3 (4500ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-2.5949993
Number of Samples:3 / 3
Experimental formaldehyde study 3 (4500ug/ml)
Bronchial epithelial cells (NHBE) treated with 4500 ug/ml formaldehyde (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 24 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control vehicle (EtOH) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 24 hours. NHBE cells were derived from a 60 year old male non-smoker.