TOP TEN perturbations for 1553318_at (Homo sapiens)
Organism: Homo sapiens
Gene: 1553318_at
Selected probe(set): 235946_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 1553318_at (235946_at) across 6537 perturbations tested by GENEVESTIGATOR:
bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)
Relative Expression (log2-ratio):3.84089Number of Samples:3 / 3
| Experimental | bronchial epithelial cell differentiation study 1 (day28) |
| Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour. | |
| Control | bronchial epithelial cell differentiation study 1 (confluent) |
| Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert. |
bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)
Relative Expression (log2-ratio):3.7928123Number of Samples:3 / 3
| Experimental | bronchial epithelial cell differentiation study 1 (day28) |
| Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour. | |
| Control | bronchial epithelial cell differentiation study 1 (subconfluent) |
| Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert. |
male infertility study 1 (mJS2) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-3.6083384Number of Samples:7 / 8
| Experimental | male infertility study 1 (mJS2) |
| Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
male infertility study 1 (mJS3) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-3.3674135Number of Samples:3 / 8
| Experimental | male infertility study 1 (mJS3) |
| Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained Sertoli cells but rarely spermatogonia. Tissue samples were classified based on modified Johnsen score (mJS) as mJS3. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-3.313405Number of Samples:2 / 8
| Experimental | male infertility study 1 (juvenile; Ad-) |
| Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
male infertility study 1 (juvenile; Ad+) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-3.2702465Number of Samples:5 / 8
| Experimental | male infertility study 1 (juvenile; Ad+) |
| Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained typical level of A-dark (Ad+) spermatogonial cells. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
male infertility study 1 (mJS5) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-2.9017363Number of Samples:8 / 8
| Experimental | male infertility study 1 (mJS5) |
| Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
brain tumor study 1 (pilocytic astrocytoma) / brain tumor study 1 (ependymoma)
Relative Expression (log2-ratio):-2.4329453Number of Samples:15 / 46
| Experimental | brain tumor study 1 (pilocytic astrocytoma) |
| Primary tumor tissue sample from the brain of patients with pilocytic astrocytoma. | |
| Control | brain tumor study 1 (ependymoma) |
| Primary tumor tissue sample from the brain of patients with ependymoma. |
brain tumor study 1 (ependymoma) / normal brain tissue
Relative Expression (log2-ratio):2.347497Number of Samples:46 / 12
| Experimental | brain tumor study 1 (ependymoma) |
| Primary tumor tissue sample from the brain of patients with ependymoma. | |
| Control | normal brain tissue |
| Histologically normal and non-neoplastic tissue sample from different brain anatomical sites of patients with primary brain tumors. |
ovarian tumor study 11 (high grade) / ovarian tumor study 11 (borderline)
Relative Expression (log2-ratio):-2.2936506Number of Samples:22 / 8
| Experimental | ovarian tumor study 11 (high grade) |
| Human microdissected tumor cells from the ovary of patients with high grade serous carcinoma. | |
| Control | ovarian tumor study 11 (borderline) |
| Human microdissected tumor cells from the ovary of patients with low-malignant (borderline) tumors of the ovary. |