TOP TEN perturbations for 1553367_a_at (Homo sapiens)
Organism: Homo sapiens
Gene: 1553367_a_at
Selected probe(set): 231457_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 1553367_a_at (231457_at) across 6537 perturbations tested by GENEVESTIGATOR:
male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-1.7780104Number of Samples:2 / 8
| Experimental | male infertility study 1 (juvenile; Ad-) |
| Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
stem cell differentiation study 50 (IRF6 shRNA; ASC; proerythroblast) / stem cell differentiation study 50 (mock shRNA; ASC; proerythroblast)
Relative Expression (log2-ratio):-1.641892Number of Samples:3 / 4
| Experimental | stem cell differentiation study 50 (IRF6 shRNA; ASC; proerythroblast) |
| Proerythroblast differentiated from IRF6 (interferon regulatory factor 6) shRNA transduced hematopoietic stem/progenitor cells (CD34+). CD34+ hematopoietic stem/progenitor cells (HSC) were transduced with lentivirus expressing shRNA targeting IRF6 gene with following differentiation to the erythroid lineage. HSCs were obtained from magnetically sorted G-CSF mobilized peripheral blood mononuclear cells of healthy donors. Cells were expanded in StemSpan SFEM medium supplied with Flt-3 ligand (100 ng/ml), SCF (100 ng/ml), IL-3 (20 ng/ml), IL-6 (20 ng/ml) and penicillin/streptomycin. Cells were harvested for total RNA extraction at the proerythroblast stage of differentiation. | |
| Control | stem cell differentiation study 50 (mock shRNA; ASC; proerythroblast) |
| Proerythroblast differentiated from control shRNA transduced hematopoietic stem/progenitor cells (CD34+). CD34+ hematopoietic stem/progenitor cells (HSC) were transduced with lentivirus expressing control shRNA with following differentiation to the erythroid lineage. HSCs were obtained from magnetically sorted G-CSF mobilized peripheral blood mononuclear cells of healthy donors. Cells were expanded in StemSpan SFEM medium supplied with Flt-3 ligand (100 ng/ml), SCF (100 ng/ml), IL-3 (20 ng/ml), IL-6 (20 ng/ml) and penicillin/streptomycin. Cells were harvested for total RNA extraction at the proerythroblast stage of differentiation. |
colorectal cancer study 45 (PDX; adenocarcinoma, NOS; metastatic) / colorectal cancer study 45 (PDX; adenocarcinoma, NOS; unstated status)
Relative Expression (log2-ratio):-1.6292858Number of Samples:17 / 2
| Experimental | colorectal cancer study 45 (PDX; adenocarcinoma, NOS; metastatic) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from a metastasis of patients with primary adenocarcinoma, NOS of the large intestine (subcutaneously implanted). Metastatic site of patient tumor sample is not reported. | |
| Control | colorectal cancer study 45 (PDX; adenocarcinoma, NOS; unstated status) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with adenocarcinoma, NOS of the large intestine (subcutaneously implanted). Primary site of the patient tumor sample is not reported. |
HIF-1a/HIF-2a depletion study 1 (normoxia; AB81) / control AB81 cell sample (normoxia)
Relative Expression (log2-ratio):-1.6074839Number of Samples:3 / 3
| Experimental | HIF-1a/HIF-2a depletion study 1 (normoxia; AB81) |
| Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with normoxia (20% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. | |
| Control | control AB81 cell sample (normoxia) |
| Human podocyte cell line AB81 treated with normoxia (20% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. |
colorectal adenoma study 2 / normal colon tissue
Relative Expression (log2-ratio):1.5918179Number of Samples:5 / 6
| Experimental | colorectal adenoma study 2 |
| Laser microdissected human adenoma sample. | |
| Control | normal colon tissue |
| Laser microdissected human colonic epithelial cells sample. |
male infertility study 1 (juvenile; Ad+) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-1.5445395Number of Samples:5 / 8
| Experimental | male infertility study 1 (juvenile; Ad+) |
| Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained typical level of A-dark (Ad+) spermatogonial cells. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
male infertility study 1 (mJS2) / normal testicular lobules tissue (mJS10)
Relative Expression (log2-ratio):-1.4349871Number of Samples:7 / 8
| Experimental | male infertility study 1 (mJS2) |
| Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2. | |
| Control | normal testicular lobules tissue (mJS10) |
| Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10). |
PMA study 6 (500ng/ml) / vehicle (DME) treated bronchial epithelial cell sample
Relative Expression (log2-ratio):1.3694954Number of Samples:3 / 17
| Experimental | PMA study 6 (500ng/ml) |
| Bronchial epithelial cells (NHBE) treated with tetradecanoylphorbol acetate (500ng/ml; vendor: Sigma / catalog number: P1585 / catalog name: Phorbol 12-myristate 13-acetate [16561-29-8]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:--- | |
| Control | vehicle (DME) treated bronchial epithelial cell sample |
| Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). |
colorectal cancer study 45 (PDX; adenocarcinoma, NOS; unstated status) / colorectal cancer study 45 (PDX; adenocarcinoma, NOS; primary)
Relative Expression (log2-ratio):1.3522186Number of Samples:2 / 73
| Experimental | colorectal cancer study 45 (PDX; adenocarcinoma, NOS; unstated status) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with adenocarcinoma, NOS of the large intestine (subcutaneously implanted). Primary site of the patient tumor sample is not reported. | |
| Control | colorectal cancer study 45 (PDX; adenocarcinoma, NOS; primary) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary adenocarcinoma, NOS of the large intestine (subcutaneously implanted). |
HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81) / hypoxia study 11 (AB81)
Relative Expression (log2-ratio):-1.3414688Number of Samples:3 / 3
| Experimental | HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81) |
| Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. | |
| Control | hypoxia study 11 (AB81) |
| Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. |