TOP TEN perturbations for 1553440_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553440_at
Selected probe(set): 1560751_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553440_at (1560751_at) across 6672 perturbations tested by GENEVESTIGATOR:

systemic lupus erythematosus study 13 (untreated) / normal PBMC sample

Relative Expression (log2-ratio):-2.9212532
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (untreated)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists.
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):2.8296003
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):2.7712317
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

chronic rhinosinusitis study 1 / normal nasal cavity epithelium sample

Relative Expression (log2-ratio):-1.7437725
Number of Samples:9 / 9
Experimental chronic rhinosinusitis study 1
Nasal cavity epithelium from patients with chronic rhinosinusitis.
Control normal nasal cavity epithelium sample
Normal nasal cavity epithelium.

chronic rhinosinusitis; cystic fibrosis study 1 / normal nasal cavity epithelium sample

Relative Expression (log2-ratio):-1.7337036
Number of Samples:5 / 9
Experimental chronic rhinosinusitis; cystic fibrosis study 1
Nasal cavity epithelium from Cystic Fibrosis patients with chronic rhinosinusitis.
Control normal nasal cavity epithelium sample
Normal nasal cavity epithelium.

pioglitazone study 4 (1uM; 6h) / normal PBMC sample

Relative Expression (log2-ratio):-1.6756887
Number of Samples:5 / 5
Experimental pioglitazone study 4 (1uM; 6h)
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects and cultured with pioglitazone. Freshly isolated PBMCs were cultured in RPMI/10% FBS in the presence of 1uM pioglitazone for 6 hours. ATC code:
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

diabetes type 2 study 27 (LCM) / diabetes type 2 study 27 (enzymatic)

Relative Expression (log2-ratio):1.579627
Number of Samples:34 / 19
Experimental diabetes type 2 study 27 (LCM)
Pancreatic islet samples obtained from type 2 diabetic (T2D) phenotyped pancreatectomized patients (PPP) and isolated by laser capture microdissection (LCM). Islets specimens were retrieved by LCM from snap-frozen surgical specimen from patients who underwent pancreatectomy for pancreatic diseases. Histopathology of the resected tissue did not reveal insulitis in any PPP. Patients age <18 years were excluded. Patients with type 2 diabetes had fasting glycemia ≥7.0 mmol/l; HbA1C ≥6.5% and history of diabetes for >1 year.
Control diabetes type 2 study 27 (enzymatic)
Pancreatic islet samples obtained from type 2 diabetic (T2D) patients and isolated by enzymatic digestion. Well-preserved islets were isolated by collagenase digestion of pancreas from brain-dead organ donors which suffered from type 2 diabetes. After 2±1 days of culture, islets were successfully hand-picked and processed for further analyses. Type 2 diabetes was diagnosed based on clinical history, treatment with glucose-lowering drugs, and lack of anti-GAD65 autoantibodies.

smoking study 66 (48h) / normal sham-exposed nasal organotypic tissue

Relative Expression (log2-ratio):-1.5769873
Number of Samples:3 / 3
Experimental smoking study 66 (48h)
Human nasal organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 48 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed nasal organotypic tissue
Human nasal organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 48 hours. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

asthma study 30 (paucigranulocytic AIP) / control nasal scrape sample (paucigranulocytic AIP)

Relative Expression (log2-ratio):-1.5693264
Number of Samples:23 / 9
Experimental asthma study 30 (paucigranulocytic AIP)
Nasal scrape samples from asthmatic adult patients with paucigranulocytic airway inflammatory phenotype (AIP).
Control control nasal scrape sample (paucigranulocytic AIP)
Control nasal scrape samples from adult subjects with paucigranulocytic airway inflammatory phenotype (AIP).

chronic obstructive pulmonary disease study 30 / normal nasal epithelium tissue (never-smoker)

Relative Expression (log2-ratio):1.5029802
Number of Samples:50 / 54
Experimental chronic obstructive pulmonary disease study 30
Nasal epithelium obtained from patients who suffered from COPD GOLD I or II (mild-to-moderate COPD) and were current smokers. Subjects had early-stage COPD (FEV during the first second [FEV1]/ forced vital capacity [FVC] 70 %) and had a smoking history of at least 10 pack/years. Subjects in each of the groups were matched to subjects in the COPD group by age (±5 years), ethnicity, and sex using a match ID.
Control normal nasal epithelium tissue (never-smoker)
Nasal epithelium obtained from never smokers (NS). Subjects in each of the groups were matched to subjects in the COPD group by age (±5 years), ethnicity, and sex using a match ID.