TOP TEN perturbations for 1553453_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553453_at
Selected probe(set): 1553453_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553453_at (1553453_at) across 6672 perturbations tested by GENEVESTIGATOR:

bisacodyl study 4 (3975ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):1.2556973
Number of Samples:3 / 17
Experimental bisacodyl study 4 (3975ng/ml)
Bronchial epithelial cells (NHBE) treated with bisacodyl (3975ng/ml; vendor: Prestwick Chemical / catalog number: 419 / catalog name: Bisacodyl [603-50-9]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:, ,
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

small cell lung cancer study 4 (XCL from CDX) / small cell lung cancer study 4 (cell line)

Relative Expression (log2-ratio):-1.2453785
Number of Samples:11 / 4
Experimental small cell lung cancer study 4 (XCL from CDX)
Xenograft-derived cell lines (XCL) established from a small cell lung carcinoma (SCLC) publicly available cell lines derived xenografts (CDX derived cell lines). Xenografts were grown in the flanks of a nude mice. Obtained cells were cultivated under conventional tissue culture conditions.
Control small cell lung cancer study 4 (cell line)
Various small cell lung cancer (SCLC) publicly available cell lines samples.

glioma study 17 (oligoastrocytoma; unsorted) / non-tumor cortical tissue

Relative Expression (log2-ratio):1.1975126
Number of Samples:2 / 4
Experimental glioma study 17 (oligoastrocytoma; unsorted)
Brain cells isolated from low grade oligoastrocytoma (grade II). Tumor tissue was dissociated using enzymatic treatment and all cells were used for RNA isolation.
Control non-tumor cortical tissue
Cortical tissue obtained from patients with epilepsy, but without any manifested brain cancer. The tissue was dissociated using enzymatic treatment, but all brain cell types were used for analyses.

Xq12–q13.3 duplication syndrome study 1 / normal lymphoblastoid cell line sample

Relative Expression (log2-ratio):1.1100435
Number of Samples:2 / 13
Experimental Xq12–q13.3 duplication syndrome study 1
Lymphoblastoid cell line samples from two brothers with Xq12–q13.3 duplication. The patients are affected by severe autism and global developmental delay with multiorgan involvement.
Control normal lymphoblastoid cell line sample
Lymphoblastoid cell line samples from healthy subjects.

rheumatoid arthritis study 37 (pbCD8cmemTC) / normal peripheral blood CD8 central memory T-cell sample

Relative Expression (log2-ratio):-1.1062307
Number of Samples:6 / 6
Experimental rheumatoid arthritis study 37 (pbCD8cmemTC)
Peripheral blood CD8 central memory T-cell samples derived from patients with rheumatoid arthritis (RA). Some of the patients were assigned to the treatment with disease-modifying antirheumatic drugs (DMARDs) i.e. tocilizumab (TCZ) and/or infliximab (IFX) and/or methotrexat (MTX). Patients characteristics: age 46.50±16.50 year; 2 males and 4 females; RF positive 5 (83.3%); ACPA positive 4 (66.7%); CRP 1.45±1.12 mg/dl; ESR 54.67±41.91 mm/hr; DAS28-CRP 4.86±0.69; DAS28-ESR 5.60±1.07; SDAI 25.12±8.82; CDAI 23.67±9.13; HAQ-DI 0.75±0.47; TJC28 7.33±2.66; SJC28 6.50±3.62; Pain, VAS 55.00±23.42; Physician GA, VAS 41.50 ±13.50 mm; Subject GA, VAS 56.83±20.68 mm
Control normal peripheral blood CD8 central memory T-cell sample
Peripheral blood CD8 central memory T-cell samples derived from healthy control subjects who did not have autoimmune diseases or were not receiving any drugs. Donor characteristics: age 43.83±6.55 year; 6 females.

medulloblastoma study 2 / non-tumor brain tissue

Relative Expression (log2-ratio):0.94288826
Number of Samples:4 / 9
Experimental medulloblastoma study 2
Primary tumor tissue sample from the infratentorial brain of pediatric patients with large-cell anaplastic medulloblastoma.
Control non-tumor brain tissue
Histologically normal brain tissue at rapid autopsy from patients who died from atypical teratoid/rhabdoid tumor.

atopic dermatitis study 12 (lesional; adults) / atopic dermatitis study 12 (lesional; children)

Relative Expression (log2-ratio):-0.897707
Number of Samples:20 / 18
Experimental atopic dermatitis study 12 (lesional; adults)
Lesional skin biopsy samples from adult patients (age range 18-73 years) with long-standing atopic dermatitis.
Control atopic dermatitis study 12 (lesional; children)
Lesional popliteal skin biopsy samples from pediatric patients (age range 3 months-5 years) with early-onset atopic dermatitis. All biopsy specimens were from chronic lesions present for more than 72 hours. All patients had moderate-to-severe disease with recent-onset (within the previous 6 months). Systemic immunosuppressants within the past 4 weeks, topical steroids or immunomodulators within 1 week, or moisturizers within 12 hours before evaluation were restricted. Patients with active skin infections were excluded.

rheumatoid arthritis study 31 / IFNa-2a study 1

Relative Expression (log2-ratio):-0.8496342
Number of Samples:4 / 7
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocytes from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control IFNa-2a study 1
Monocyte samples isolated from healthy donors and stimulated in vitro by 100 ng/ml IFNα2a in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

memory B cell study 1 (CD27low) / memory B cell study 1 (undivided)

Relative Expression (log2-ratio):-0.84558296
Number of Samples:6 / 6
Experimental memory B cell study 1 (CD27low)
CD27 enriched proliferating human memory B cells expressing low level of CD27 (marker of antibody secretion) activated with CpG oligodeoxynucleotide (10 ng/ml), recombinant human cytokines IL-2 (20 IU/ml), IL-10 (50 ng/ml), IL-15 (10 ng/ml), recombinant human BAFF (75 ng/ml) in PC-L medium (IMDM medium, lacromin (50mg/ml), insulin (5mg/ml), penicillin/streptomycin, gentamicin (15mg/ml), normocin (0.1% v/v), 10% FCS) for 80 hours.
Control memory B cell study 1 (undivided)
CD27 enriched undivided human memory B cells (expressing low level of CD27) activated with CpG oligodeoxynucleotide (10 ng/ml), recombinant human cytokines IL-2 (20 IU/ml), IL-10 (50 ng/ml), IL-15 (10 ng/ml), recombinant human BAFF (75 ng/ml) in PC-L medium (IMDM medium, lacromin (50mg/ml), insulin (5mg/ml), penicillin/streptomycin, gentamicin (15mg/ml), normocin (0.1% v/v), 10% FCS) for 80 hours.

myotonic dystrophy study 4 (deltoid muscle; DM-like) / normal skeletal muscle tisuue (fetal)

Relative Expression (log2-ratio):0.84213257
Number of Samples:3 / 3
Experimental myotonic dystrophy study 4 (deltoid muscle; DM-like)
Deltoid muscle biopsies obtained from patients with myotonic-like dystrophy (DMx; DM-like; no DMPK or ZNF9 expansion).
Control normal skeletal muscle tisuue (fetal)
Commercially purchased normal fetal skeletal muscle tissue samples.