TOP TEN perturbations for 1553472_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553472_at
Selected probe(set): 1553472_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553472_at (1553472_at) across 6672 perturbations tested by GENEVESTIGATOR:

systemic lupus erythematosus study 13 (untreated) / normal PBMC sample

Relative Expression (log2-ratio):-5.016695
Number of Samples:4 / 5
Experimental systemic lupus erythematosus study 13 (untreated)
Peripheral blood mononuclear cells (PBMCs) obtained from systemic lupus erythematosus (SLE) patients. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours. Lupus patients fulfilled American College of Rheumatology classification criteria for disease, and disease activity was quantified by SLEDAI. Patients were excluded if they showed symptoms of recent or active infection or were pregnant. None of the patients was taking Pioglitazone or other PPAR-γ agonists.
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

pioglitazone study 4 (1uM; 6h) / normal PBMC sample

Relative Expression (log2-ratio):-2.7002697
Number of Samples:5 / 5
Experimental pioglitazone study 4 (1uM; 6h)
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects and cultured with pioglitazone. Freshly isolated PBMCs were cultured in RPMI/10% FBS in the presence of 1uM pioglitazone for 6 hours. ATC code:
Control normal PBMC sample
Peripheral blood mononuclear cells (PBMCs) obtained from healthy subjects. Freshly isolated PBMCs were cultured in RPMI/10% FBS for 6 hours.

systemic lupus erythematosus study 3 / normal blood sample

Relative Expression (log2-ratio):2.0968728
Number of Samples:89 / 30
Experimental systemic lupus erythematosus study 3
Blood samples derived from patients with anti-ribonuclear protein-positive (anti-RNP+) systemic lupus erythematosus (SLE).
Control normal blood sample
Blood samples from healthy individuals.

norethisterone study 4 (3999ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):1.6545086
Number of Samples:3 / 17
Experimental norethisterone study 4 (3999ng/ml)
Bronchial epithelial cells (NHBE) treated with norethisterone (3999ng/ml; vendor: Prestwick Chemical / catalog number: Prestw-253 / catalog name: Norethindrone [68-22-4]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:,
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

cholesterol study 1 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-1.4074678
Number of Samples:3 / 17
Experimental cholesterol study 1
Bronchial epithelial cells (NHBE) treated with cholesterol (2000 ng/ml; vendor: Sigma / catalog number: C3045 / catalog name: Cholesterol [57-88-5]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

IGF2 study 1 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-1.3969913
Number of Samples:3 / 17
Experimental IGF2 study 1
Bronchial epithelial cells (NHBE) treated with 100 ng/ml recombinant human insulin-like growth factor II (IGF2; vendor: PeproTech / catalog number: 100-12 / catalog name: IGF-II) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

HCC study 26 (tumor; TG/GG genotype) / HCC study 26 (non-tumor; TG/GG genotype)

Relative Expression (log2-ratio):-1.2623615
Number of Samples:10 / 10
Experimental HCC study 26 (tumor; TG/GG genotype)
Liver tumor tissue resected from patients with hepatocellular carcinoma, with Interleukin-28B (Il28B) rs8099917 TG/GG (minor) genotype. HCC diagnosis was based predominantly on image analysis (CT and/or MRI and abdominal angiography with CT imaging in the arterial and portal flow phase). Patients inclusion criteria were: a) Child-Pugh class A or B; b) the presence of up to 3 tumors, each 3 cm or less; c) HCV infection (positive for HCV RNA, patients with sustained viral response were excluded); d) radical treatment by either surgical resection or RFA; and e) availability of blood samples for genetic analyses. Patients clinical features were: Sex (male:female) 1:9; age: 60 (49-75); cirrhosis (yes:no) 5:5; History of IFN therapy (yes:no) 5:5; Child-Pugh class (A:B) 10:0; Tumor no. (1:2:3) 7:0:3; Tumor size (mm) 28.5 (17-38).
Control HCC study 26 (non-tumor; TG/GG genotype)
Liver non-tumor tissue resected from patients with hepatocellular carcinoma, with Interleukin-28B (Il28B) rs8099917 TG/GG (minor) genotype. HCC diagnosis was based predominantly on image analysis (CT and/or MRI and abdominal angiography with CT imaging in the arterial and portal flow phase). Patients inclusion criteria were: a) Child-Pugh class A or B; b) the presence of up to 3 tumors, each 3 cm or less; c) HCV infection (positive for HCV RNA, patients with sustained viral response were excluded); d) radical treatment by either surgical resection or RFA; and e) availability of blood samples for genetic analyses. Patients clinical features were: Sex (male:female) 1:9; age: 60 (49-75); cirrhosis (yes:no) 5:5; History of IFN therapy (yes:no) 5:5; Child-Pugh class (A:B) 10:0; Tumor no. (1:2:3) 7:0:3; Tumor size (mm) 28.5 (17-38).

CpG ODN study 1 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-1.251226
Number of Samples:3 / 17
Experimental CpG ODN study 1
Bronchial epithelial cells (NHBE) treated with CpG oligodeoxynucleotides (18259 ng/ml; vendor: InvivoGen / catalog number: tlrl-2006 / catalog name: ODN 2006 (ODN 7909)) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

betahistine study 4 (5648ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):1.2489748
Number of Samples:3 / 17
Experimental betahistine study 4 (5648ng/ml)
Bronchial epithelial cells (NHBE) treated with betahistine (5648ng/ml; vendor: Prestwick Chemical / catalog number: 543 / catalog name: Betahistine mesylate [54856-23-4]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

NTF3 study 1 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):-1.2330027
Number of Samples:3 / 17
Experimental NTF3 study 1
Bronchial epithelial cells (NHBE) treated with 200 ng/ml recombinant human neurotrophin-3 (NTF3; vendor: PeproTech / catalog number: 450-03 / catalog name: NT-3) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).