TOP TEN perturbations for 1553540_a_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553540_a_at
Selected probe(set): 204717_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553540_a_at (204717_s_at) across 6674 perturbations tested by GENEVESTIGATOR:

succinate dehydrogenase B depletion study 1 (siRNA) / control siRNA transfected Hep3B cell sample

Relative Expression (log2-ratio):-2.6961164
Number of Samples:3 / 3
Experimental succinate dehydrogenase B depletion study 1 (siRNA)
Hep3B cells stably transfected with short hairpin vectors containing an siRNA directed to the subunit B of the succinate dehydrogenase.
Control control siRNA transfected Hep3B cell sample
Hep3B cells stably transfected with empty siRNA expression vector (pU6).

ARC study 1 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):-2.644248
Number of Samples:2 / 2
Experimental ARC study 1
MCF7 cells treated with 2uM ARC (4-amino-6-hydrazino-7-beta-d-ribofuranosyl-7H-pyrrolo-(2, 3-d)-pyrimidine-5-carboxamide, NSC 188491) for 24 hours. ATC code:---
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

ketoconazole study 6 (15uM) / vehicle (DMSO) treated hepatocyte sample

Relative Expression (log2-ratio):2.4827347
Number of Samples:2 / 2
Experimental ketoconazole study 6 (15uM)
Hepatocytes treated with compound: ketoconazole (15uM; CHEMBL75) for 24 hours. ATC code:, ,
Control vehicle (DMSO) treated hepatocyte sample
Hepatocytes treated with vehicle (DMSO) for 24 hours.

ovarian tumor study 14 / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):2.4763355
Number of Samples:4 / 4
Experimental ovarian tumor study 14
Human pooled cancer samples from the ovary of patients with moderate and poorly differentiated serous carcinoma of the ovary.
Control normal ovarian surface epithelial cell sample
Human epithelial cell samples from histopathological normal and non-cancerous ovary tissue from donors with non-cancerous, benign gynecological diseases.

Hep-G2 / HepaRG

Relative Expression (log2-ratio):2.2647858
Number of Samples:9 / 12
Experimental Hep-G2
Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code:
Control HepaRG
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code:

gefitinib study 2 / wild type A431 cell sample

Relative Expression (log2-ratio):2.0748606
Number of Samples:3 / 3
Experimental gefitinib study 2
Gefitinib-resistant A431 (A431 GR) cells generated by growing parental cells in the presence of increasing concentrations of gefitinib up to 3 μM. Cells were selected and maintained in gefitinib (3μM). ATC code:
Control wild type A431 cell sample
A431 wild type cells.

doxorubicin study 3 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):-2.0256805
Number of Samples:2 / 2
Experimental doxorubicin study 3
MCF7 cells treated with 2uM doxorubicin for 24 hours. ATC code:
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

colorectal cancer study 43 (metastase; brain) / colorectal cancer study 43 (metastase; lung)

Relative Expression (log2-ratio):1.9959774
Number of Samples:2 / 5
Experimental colorectal cancer study 43 (metastase; brain)
Metastatic tumor tissue obtained from the brain of patients with primary colorectal adenocarcinoma.
Control colorectal cancer study 43 (metastase; lung)
Metastatic tumor tissue obtained from the lung of patients with primary colon adenocarcinoma.

tumor supernatant activation study 3 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):-1.9622183
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

dysferlinopathy study 2 (LGMD2B; severe) / normal vastus lateralis tissue

Relative Expression (log2-ratio):-1.9576817
Number of Samples:2 / 6
Experimental dysferlinopathy study 2 (LGMD2B; severe)
Biopsies of vastus lateralis muscle from male patients with limb-girdle muscular dystrophy 2B (LGMD2B) and severe fibrotic replacement. All subjects showed evidence of a dystrophic process (degeneration/regeneration of muscle fibers) and dysferlin mutation.
Control normal vastus lateralis tissue
Biopsies of vastus lateralis muscle from young adult volunteers. Samples were taken as normal controls from baseline samples of exercise experiments.