TOP TEN perturbations for 1553551_s_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553551_s_at
Selected probe(set): 1553551_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553551_s_at (1553551_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-3.4165726
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-3.1548557
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):2.8021488
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

idiopathic pulmonary fibrosis study 9 (non-contractile; ribo. bound RNA) / idiopathic pulmonary fibrosis study 9 (non-contractile; total RNA)

Relative Expression (log2-ratio):-2.7859526
Number of Samples:6 / 6
Experimental idiopathic pulmonary fibrosis study 9 (non-contractile; ribo. bound RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control idiopathic pulmonary fibrosis study 9 (non-contractile; total RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

idiopathic pulmonary fibrosis study 9 (contractile; ribo. bound RNA) / idiopathic pulmonary fibrosis study 9 (contractile; total RNA)

Relative Expression (log2-ratio):-2.7227793
Number of Samples:6 / 6
Experimental idiopathic pulmonary fibrosis study 9 (contractile; ribo. bound RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate the environment of physiological healing, the 3-dimensional type I collagen gel was released from the border of the dish to allow primary cells to freely contract (contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control idiopathic pulmonary fibrosis study 9 (contractile; total RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate the environment of physiological healing, the 3-dimensional type I collagen gel was released from the border of the dish to allow primary cells to freely contract (contractile matrices). Microarray was performed with total RNA.

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):2.6932735
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):2.6747131
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):2.6535206
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):2.547987
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-2.0456123
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

Organism: Homo sapiens
Gene: 1553551_s_at
Selected probe(set): 1553551_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553551_s_at (1553551_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-3.4165726
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-3.1548557
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):2.8021488
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

idiopathic pulmonary fibrosis study 9 (non-contractile; ribo. bound RNA) / idiopathic pulmonary fibrosis study 9 (non-contractile; total RNA)

Relative Expression (log2-ratio):-2.7859526
Number of Samples:6 / 6
Experimental idiopathic pulmonary fibrosis study 9 (non-contractile; ribo. bound RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control idiopathic pulmonary fibrosis study 9 (non-contractile; total RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

idiopathic pulmonary fibrosis study 9 (contractile; ribo. bound RNA) / idiopathic pulmonary fibrosis study 9 (contractile; total RNA)

Relative Expression (log2-ratio):-2.7227793
Number of Samples:6 / 6
Experimental idiopathic pulmonary fibrosis study 9 (contractile; ribo. bound RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate the environment of physiological healing, the 3-dimensional type I collagen gel was released from the border of the dish to allow primary cells to freely contract (contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control idiopathic pulmonary fibrosis study 9 (contractile; total RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate the environment of physiological healing, the 3-dimensional type I collagen gel was released from the border of the dish to allow primary cells to freely contract (contractile matrices). Microarray was performed with total RNA.

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):2.6932735
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):2.6747131
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):2.6535206
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):2.547987
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-2.0456123
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).